Click here to switch to the map view.

The map label for this gene is hisIE [H]

Identifier: 157161519

GI number: 157161519

Start: 2142469

End: 2143077

Strand: Direct

Name: hisIE [H]

Synonym: EcHS_A2165

Alternate gene names: 157161519

Gene position: 2142469-2143077 (Clockwise)

Preceding gene: 157161518

Following gene: 157161551

Centisome position: 46.14

GC content: 52.87

Gene sequence:

>609_bases
ATGTTAACAGAACAACAACGTCACGAACTGGACTGGGAAAAAACCGACGGACTGATGCCGGTGATTGTACAACACGCGGT
ATCTGGCGAAGTGTTAATGCTGGGCTATATGAACCCGGAAGCTCTGGACAAAACTATCGAAAGCGGCAAAGTCACCTTCT
TCTCGCGAACTAAACAACGGCTGTGGACCAAAGGCGAAACGTCTGGCAATTTTCTCAATGTGGTGAGTATTGCCCCGGAC
TGCGACAACGACACGTTACTGGTGCTGGCGAATCCCATCGGGCCAACCTGCCACAAAGGCACCAGCAGCTGCTTCGGCGA
CACCGCTCACCAGTGGCTGTTCCTGTATCAACTGGAACAACTGCTCGCCGAACGCAAATCTGCCGACCCGGAAACCTCTT
ACACAGCCAAACTGTATGCCAGCGGCACCAAACGCATTGCGCAGAAAGTGGGCGAAGAAGGGGTGGAAACCGCACTGGCC
GCGACGGTAAATGACCGCTTTGAGCTGACCAACGAGGCATCTGATTTGATGTATCACCTGCTGGTGCTGTTGCAGGATCA
GGGGCTGGATCTCGGAGAAGTGATTGATAATCTGAAGAATCGGCATTAA

Upstream 100 bases:

>100_bases
ACGTTGACGGTGCGCTGGCAGCTTCTGTATTCCACAAACAAATAATCAATATTAGTGAATTAAAAGCGTACCTGGCAACA
CAGGGCGTGGAGATCAGGAT

Downstream 100 bases:

>100_bases
CGCGTTGGCCCCCGCAGCAACGGCGGGGGTAGCAGCTCTCAGGATTTGCTTTCCAGCAATGTAGTGTAGAGATTGACATA
GGCGTCAATCATATGGTCGG

Product: bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase protein

Products: NA

Alternate protein names: Phosphoribosyl-AMP cyclohydrolase; PRA-CH; Phosphoribosyl-ATP pyrophosphatase; PRA-PH [H]

Number of amino acids: Translated: 202; Mature: 202

Protein sequence:

>202_residues
MLTEQQRHELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDKTIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPD
CDNDTLLVLANPIGPTCHKGTSSCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
ATVNDRFELTNEASDLMYHLLVLLQDQGLDLGEVIDNLKNRH

Sequences:

>Translated_202_residues
MLTEQQRHELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDKTIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPD
CDNDTLLVLANPIGPTCHKGTSSCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
ATVNDRFELTNEASDLMYHLLVLLQDQGLDLGEVIDNLKNRH
>Mature_202_residues
MLTEQQRHELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDKTIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPD
CDNDTLLVLANPIGPTCHKGTSSCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
ATVNDRFELTNEASDLMYHLLVLLQDQGLDLGEVIDNLKNRH

Specific function: Histidine biosynthesis; second step. Histidine biosynthesis; third step. [C]

COG id: COG0139

COG function: function code E; Phosphoribosyl-AMP cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PRA-PH family [H]

Homologues:

Organism=Escherichia coli, GI2367128, Length=202, Percent_Identity=96.039603960396, Blast_Score=405, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6319819, Length=204, Percent_Identity=34.8039215686275, Blast_Score=99, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023019
- InterPro:   IPR008179
- InterPro:   IPR021130
- InterPro:   IPR002496
- ProDom:   PD002610 [H]

Pfam domain/function: PF01502 PRA-CH; PF01503 PRA-PH [H]

EC number: =3.5.4.19; =3.6.1.31 [H]

Molecular weight: Translated: 22514; Mature: 22514

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTEQQRHELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDKTIESGKVTFFSRTKQR
CCCCCHHHCCCCHHCCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCEEEHHHHHHH
LWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTSSCFGDTAHQWLFLYQLEQ
HHCCCCCCCCEEEEEEECCCCCCCEEEEEECCCCCCCCCCCCHHHCHHHHHHHHHHHHHH
LLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALAATVNDRFELTNEASDLMYHL
HHHHHHCCCCCCCCEEHHHHHHHHHHHHHHCHHHHHHHHHHHHCCHHHHCCHHHHHHHHH
LVLLQDQGLDLGEVIDNLKNRH
HHHHHCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MLTEQQRHELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDKTIESGKVTFFSRTKQR
CCCCCHHHCCCCHHCCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCEEEHHHHHHH
LWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTSSCFGDTAHQWLFLYQLEQ
HHCCCCCCCCEEEEEEECCCCCCCEEEEEECCCCCCCCCCCCHHHCHHHHHHHHHHHHHH
LLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALAATVNDRFELTNEASDLMYHL
HHHHHHCCCCCCCCEEHHHHHHHHHHHHHHCHHHHHHHHHHHHCCHHHHCCHHHHHHHHH
LVLLQDQGLDLGEVIDNLKNRH
HHHHHCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10222209; 11206551; 11258796 [H]