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The map label for this gene is hisA [H]

Identifier: 157161517

GI number: 157161517

Start: 2140980

End: 2141717

Strand: Direct

Name: hisA [H]

Synonym: EcHS_A2163

Alternate gene names: 157161517

Gene position: 2140980-2141717 (Clockwise)

Preceding gene: 157161516

Following gene: 157161518

Centisome position: 46.11

GC content: 56.78

Gene sequence:

>738_bases
ATGATTATTCCGGCATTAGATTTAATCGACGGCACCGTGGTGCGTCTCCATCAGGGCGATTACGGCAAACAGCGCGATTA
CGGTAACGACCCGCTGCCACGCTTACAGGATTACGCCGCGCAGGGTGCCGAAGTGTTGCATCTGGTGGATCTGACCGGGG
CAAAAGATCCGGCTAAACGTCAAATCCCGCTGATTAAAACCCTGGTCGCGGGCGTTAACGTTCCGGTGCAGGTTGGCGGC
GGCGTGCGTAGCGAAGAAGACGTGGCGGCGTTACTGGAAGCGGGCGTTGCGCGCGTGGTGGTTGGCTCCACCGCGGTGAA
ATCACCAGAAATGGTGAAAGGCTGGTTTGAACGCTTCGGTGCCGATGCCTTAGTGCTGGCGCTGGATGTCCGTATTGACG
AGCAAGGCAACAAGCAGGTGGCAGTCAGCGGCTGGCAAGAGAACTCGGGCGTTTCACTGGAACAACTGGTGGAAACCTAT
CTGCCCGTCGGCCTGAAACATGTGCTGTGTACCGATATCTCGCGCGACGGCACGCTGGCAGGTTCTAACGTCTCTTTATA
TGAAGAAGTGTGCGCCAGATATCCGCAGGTGGCATTTCAGTCTTCCGGCGGTATTGGCGACATTGATGATGTCGCGGCCC
TGCGTGGTACTGGCGTGCGCGGCGTAATAGTTGGTCGTGCATTACTGGAAGGTAAATTCACCGTGAAGGAGGCCATCGCA
TGCTGGCAAAACGCATAA

Upstream 100 bases:

>100_bases
CCGCGGCGGTACAAAAAGATAACTTCTACGGCGTGCAGTTCCACCCGGAGCGTTCTGGTGCTGCTGGCGCTAATTTGCTG
AAAAACTTCCTGGAGATGTG

Downstream 100 bases:

>100_bases
TCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATTGTA
CCGCTGGCAAAACGCTACGC

Product: 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase

Products: NA

Alternate protein names: Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase [H]

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAEVLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGG
GVRSEEDVAALLEAGVARVVVGSTAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDIDDVAALRGTGVRGVIVGRALLEGKFTVKEAIA
CWQNA

Sequences:

>Translated_245_residues
MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAEVLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGG
GVRSEEDVAALLEAGVARVVVGSTAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDIDDVAALRGTGVRGVIVGRALLEGKFTVKEAIA
CWQNA
>Mature_245_residues
MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAEVLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGG
GVRSEEDVAALLEAGVARVVVGSTAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDIDDVAALRGTGVRGVIVGRALLEGKFTVKEAIA
CWQNA

Specific function: Histidine biosynthesis; fourth step. [C]

COG id: COG0106

COG function: function code E; Phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide (ProFAR) isomerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI87082028, Length=245, Percent_Identity=99.1836734693878, Blast_Score=485, Evalue=1e-139,
Organism=Escherichia coli, GI1788336, Length=231, Percent_Identity=26.4069264069264, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR006063
- InterPro:   IPR023016
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: =5.3.1.16 [H]

Molecular weight: Translated: 26016; Mature: 26016

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAEVLHLVDLTGAKDPAKR
CCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCEEEEEEECCCCCCCCHH
QIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVGSTAVKSPEMVKGWFERFG
CCHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHCHHHEEECCHHCCCHHHHHHHHHHHC
ADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETYLPVGLKHVLCTDISRDGTLA
CCEEEEEEEEEECCCCCCEEEEECCCCCCCCCHHHHHHHHHCCCHHHHHHHCCCCCCCCC
GSNVSLYEEVCARYPQVAFQSSGGIGDIDDVAALRGTGVRGVIVGRALLEGKFTVKEAIA
CCCCHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHH
CWQNA
HHCCC
>Mature Secondary Structure
MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAEVLHLVDLTGAKDPAKR
CCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCEEEEEEECCCCCCCCHH
QIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVGSTAVKSPEMVKGWFERFG
CCHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHCHHHEEECCHHCCCHHHHHHHHHHHC
ADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETYLPVGLKHVLCTDISRDGTLA
CCEEEEEEEEEECCCCCCEEEEECCCCCCCCCHHHHHHHHHCCCHHHHHHHCCCCCCCCC
GSNVSLYEEVCARYPQVAFQSSGGIGDIDDVAALRGTGVRGVIVGRALLEGKFTVKEAIA
CCCCHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHH
CWQNA
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA