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The map label for this gene is php [H]

Identifier: 157161479

GI number: 157161479

Start: 2105843

End: 2106673

Strand: Direct

Name: php [H]

Synonym: EcHS_A2119

Alternate gene names: 157161479

Gene position: 2105843-2106673 (Clockwise)

Preceding gene: 157161469

Following gene: 157161481

Centisome position: 45.35

GC content: 48.5

Gene sequence:

>831_bases
ATGGACCGCAAACCTATTGAAGATGTGATCTTCGAAATTAATAACTTTATCTCGCTCGGTGGTCGCACCATTGTTGATGC
CACAGGGTCTGAGTCTATCGGACGAGATGCGCAGGCATTACGAGAAGTCGCATTAAAAACAGGTCTGAATATTGTTGCTT
CCTCCGGTCCCTACCTGGAAAAATTTGAAAGCCAGAGAATTCATAAAACGGTTGATGAACTGGCGGCAACGATTGATAAA
GAATTGAATCAAGGGATTGGCGATACGGATATTCGTGCCGGAATGATCGGTGAAATAGGTGTCTCACCGACATTTACCGA
AGCCGAGCATAACAGCTTGCGGGCTGCCTCGCTGGCACAGATTAACAATCCTCATGTGGCGATGAATATTCACATGCCGG
GCTGGCTTCGTCGCGGTGATGAAGTACTCGACATTGTGTTAGGTGAAATGGGCGTCTCGCCAAATAAAGTCTCTCTCGCA
CACTCGGATCCGTCAGGAAAAGACGTGGCGTATCAGCGGAAAATGCTTGATAAGGGTGTCTGGCTGGAATTCGACATGAT
TGGCCTAGACATTACCTTCCCGAAAGAGGGAATAGCGCCAGGGGTGCAGGAGACTGCCGATGCCGTCGCTCATCTCATTG
AGTTGGGATACGCCGATCAGCTTGTTCTCAGCCACGATGTCTTCCTTAAACAAATGTGGGCTAAAAATGGCGGTAATGGC
TGGGGATTTGTTCCAGATGTTTTTCTGGCCTATCTGGCGGAGCGCGGCGTCGATAAAACGATCCTCAAAAAACTCTGTAT
CGATAATCCCGGACGATTATTGACCGCATAA

Upstream 100 bases:

>100_bases
ATCGCAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGC
GGTGATCCTACCCACGTAAT

Downstream 100 bases:

>100_bases
AACCATTATCCCCCGGCACACTGCGCCGGGGGATGTCAATTAATACCCCGCCGTTAAATCATCCACCAAGCGCGAGTCGG
ATGCGCCGTACAACTCACCA

Product: phosphotriesterase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MDRKPIEDVIFEINNFISLGGRTIVDATGSESIGRDAQALREVALKTGLNIVASSGPYLEKFESQRIHKTVDELAATIDK
ELNQGIGDTDIRAGMIGEIGVSPTFTEAEHNSLRAASLAQINNPHVAMNIHMPGWLRRGDEVLDIVLGEMGVSPNKVSLA
HSDPSGKDVAYQRKMLDKGVWLEFDMIGLDITFPKEGIAPGVQETADAVAHLIELGYADQLVLSHDVFLKQMWAKNGGNG
WGFVPDVFLAYLAERGVDKTILKKLCIDNPGRLLTA

Sequences:

>Translated_276_residues
MDRKPIEDVIFEINNFISLGGRTIVDATGSESIGRDAQALREVALKTGLNIVASSGPYLEKFESQRIHKTVDELAATIDK
ELNQGIGDTDIRAGMIGEIGVSPTFTEAEHNSLRAASLAQINNPHVAMNIHMPGWLRRGDEVLDIVLGEMGVSPNKVSLA
HSDPSGKDVAYQRKMLDKGVWLEFDMIGLDITFPKEGIAPGVQETADAVAHLIELGYADQLVLSHDVFLKQMWAKNGGNG
WGFVPDVFLAYLAERGVDKTILKKLCIDNPGRLLTA
>Mature_276_residues
MDRKPIEDVIFEINNFISLGGRTIVDATGSESIGRDAQALREVALKTGLNIVASSGPYLEKFESQRIHKTVDELAATIDK
ELNQGIGDTDIRAGMIGEIGVSPTFTEAEHNSLRAASLAQINNPHVAMNIHMPGWLRRGDEVLDIVLGEMGVSPNKVSLA
HSDPSGKDVAYQRKMLDKGVWLEFDMIGLDITFPKEGIAPGVQETADAVAHLIELGYADQLVLSHDVFLKQMWAKNGGNG
WGFVPDVFLAYLAERGVDKTILKKLCIDNPGRLLTA

Specific function: Its real enzymatic activity is not yet known. It was tested for general esterase, aminopeptidase, sulfatase, phosphatase, carbonic anhydrase, phosphodiesterase, and phosphotriesterase activities with the following substrates:P- nitrophenyl acetate, L-alan

COG id: COG1735

COG function: function code R; Predicted metal-dependent hydrolase with the TIM-barrel fold

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphotriesterase family [H]

Homologues:

Organism=Homo sapiens, GI20070186, Length=270, Percent_Identity=29.2592592592593, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI47933341, Length=270, Percent_Identity=29.2592592592593, Blast_Score=107, Evalue=1e-23,
Organism=Escherichia coli, GI1789780, Length=265, Percent_Identity=28.3018867924528, Blast_Score=100, Evalue=8e-23,
Organism=Drosophila melanogaster, GI24645325, Length=262, Percent_Identity=22.9007633587786, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017947
- InterPro:   IPR001559 [H]

Pfam domain/function: PF02126 PTE [H]

EC number: NA

Molecular weight: Translated: 30060; Mature: 30060

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRKPIEDVIFEINNFISLGGRTIVDATGSESIGRDAQALREVALKTGLNIVASSGPYLE
CCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCEEEECCCCHHH
KFESQRIHKTVDELAATIDKELNQGIGDTDIRAGMIGEIGVSPTFTEAEHNSLRAASLAQ
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
INNPHVAMNIHMPGWLRRGDEVLDIVLGEMGVSPNKVSLAHSDPSGKDVAYQRKMLDKGV
CCCCEEEEEECCCHHHHCCHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHCCCC
WLEFDMIGLDITFPKEGIAPGVQETADAVAHLIELGYADQLVLSHDVFLKQMWAKNGGNG
EEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCC
WGFVPDVFLAYLAERGVDKTILKKLCIDNPGRLLTA
CCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCEECC
>Mature Secondary Structure
MDRKPIEDVIFEINNFISLGGRTIVDATGSESIGRDAQALREVALKTGLNIVASSGPYLE
CCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCEEEECCCCHHH
KFESQRIHKTVDELAATIDKELNQGIGDTDIRAGMIGEIGVSPTFTEAEHNSLRAASLAQ
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
INNPHVAMNIHMPGWLRRGDEVLDIVLGEMGVSPNKVSLAHSDPSGKDVAYQRKMLDKGV
CCCCEEEEEECCCHHHHCCHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHCCCC
WLEFDMIGLDITFPKEGIAPGVQETADAVAHLIELGYADQLVLSHDVFLKQMWAKNGGNG
EEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCC
WGFVPDVFLAYLAERGVDKTILKKLCIDNPGRLLTA
CCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 9548740 [H]