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The map label for this gene is yedX [H]

Identifier: 157161438

GI number: 157161438

Start: 2058056

End: 2058469

Strand: Direct

Name: yedX [H]

Synonym: EcHS_A2071

Alternate gene names: 157161438

Gene position: 2058056-2058469 (Clockwise)

Preceding gene: 157161435

Following gene: 157161439

Centisome position: 44.32

GC content: 42.03

Gene sequence:

>414_bases
ATGTTAAAGCGTTATTTAGTACTCTCGGTAGCAACGGCAGCATTTTCATTACCTTCTTTGGTTTATGCCGCACAACAAAA
CATTCTTAGCGTGCACATTTTGAACCAGCAAACCGGAAAACCCGCTGCTGACGTGACAGTCACTCTTGAAAAGAAGGCGG
ATAACGGCTGGTTACAACTTAATACCGCCAAAACAGATAAGGATGGACGAATTAAGGCACTGTGGCCCGAGCAAACTGCA
ACTACGGGCGATTACCGTGTCGTATTTAAAACCGGGGACTATTTCAAGAAACAAAATCTTGAAAGTTTCTTCCCTGAGAT
CCCCGTTGAATTTCATATTAATAAAGTGAACGAGCATTATCATGTGCCTTTATTACTTAGCCAATATGGGTATTCAACCT
ATCGTGGCAGTTAA

Upstream 100 bases:

>100_bases
AAACAGCCTGGTTCATTACAAAATTGTAATGCTGCTGTAAGGTTACCCTGGCCGCTTTTTCGCTATCCTCAAAACTCATT
CACATGACAAGGATATAAAC

Downstream 100 bases:

>100_bases
TGCATAAATATGTAAAAGCAGATCTCTGCTACTGTTAGGGAGGACACTCCCTGACAGATTAACAGTAAACGGCTCTTGCT
GGCTAACGACAAAAAAGTGT

Product: transthyretin family protein

Products: NA

Alternate protein names: HIU hydrolase; HIUHase; Transthyretin-like protein; TLP [H]

Number of amino acids: Translated: 137; Mature: 137

Protein sequence:

>137_residues
MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAADVTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTA
TTGDYRVVFKTGDYFKKQNLESFFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS

Sequences:

>Translated_137_residues
MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAADVTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTA
TTGDYRVVFKTGDYFKKQNLESFFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS
>Mature_137_residues
MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAADVTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTA
TTGDYRVVFKTGDYFKKQNLESFFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS

Specific function: Catalyzes the hydrolysis of 5-hydroxyisourate (HIU) to 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline (OHCU) [H]

COG id: COG2351

COG function: function code R; Transthyretin-like protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transthyretin family. 5-hydroxyisourate hydrolase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788281, Length=137, Percent_Identity=99.2700729927007, Blast_Score=282, Evalue=7e-78,
Organism=Caenorhabditis elegans, GI115534555, Length=137, Percent_Identity=37.956204379562, Blast_Score=101, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI115532920, Length=109, Percent_Identity=40.3669724770642, Blast_Score=97, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI115532922, Length=109, Percent_Identity=40.3669724770642, Blast_Score=97, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24652571, Length=110, Percent_Identity=40, Blast_Score=85, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014306
- InterPro:   IPR000895 [H]

Pfam domain/function: PF00576 Transthyretin [H]

EC number: =3.5.2.17 [H]

Molecular weight: Translated: 15509; Mature: 15509

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: PS00768 TRANSTHYRETIN_1 ; PS00769 TRANSTHYRETIN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAADVTVTLEKKADNGWLQL
CCCCEEEEEHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCEEEEEEECCCCCEEEE
NTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLESFFPEIPVEFHINKVNEHY
EECCCCCCCCEEEECCCCCCCCCCEEEEEECCCCHHHCCHHHHCCCCCEEEEEEECCCEE
HVPLLLSQYGYSTYRGS
ECEEEEECCCCCCCCCC
>Mature Secondary Structure
MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAADVTVTLEKKADNGWLQL
CCCCEEEEEHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCEEEEEEECCCCCEEEE
NTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLESFFPEIPVEFHINKVNEHY
EECCCCCCCCEEEECCCCCCCCCCEEEEEECCCCHHHCCHHHHCCCCCEEEEEEECCCEE
HVPLLLSQYGYSTYRGS
ECEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]