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The map label for this gene is yeaI [H]

Identifier: 157161247

GI number: 157161247

Start: 1888362

End: 1889837

Strand: Direct

Name: yeaI [H]

Synonym: EcHS_A1871

Alternate gene names: 157161247

Gene position: 1888362-1889837 (Clockwise)

Preceding gene: 157161246

Following gene: 157161248

Centisome position: 40.67

GC content: 38.21

Gene sequence:

>1476_bases
ATGATTCAGTCAACACGTATTTCCATGGGGTTATTCTTTAAATATTTTTTATCGTTAACGAAAATTGATCTTGGTCAAAA
CTATATATCTCTGCCATCAATAAAATCCAGCACTCACATTGCTCTCCTTTTTATGGTTTCTATGGGTACACAAAAATTAA
AAGCTCAAAGCTTTTTTATTTTCAGTTTATTGCTGACGTTAATTTTATTTTGCATTACTACCTTATATAACGAAAACACA
AATGTAAAACTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATTTTTCTTTT
CATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTAGCCTTATCT
ATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACTGATCCAGACAAAGTCGAATGATGTTTCT
ATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCACTCTTTTGTTATGGAAAAGACAACATCCT
TGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATTCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATC
TGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGACTACTGTCCTGACAACCATACTGCGACCTGGGGAATCAAC
TATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGTACACGATTAGCCAGCGAACTATG
GCCATTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTACACCA
TCTGGTACATCAGTCGCGGGATTGAAGTCTCCAGTAAACTGTTTGTTGTGTCTTTTCTGATTTATAACATTTTTCAGGAA
CTGCAACTCTCCAGCAAACTGGCAGTTCATGATGTGCTGACCAATATTTATAATCGGCGCTACTTTTTCAACAGCGTAGA
GTCATTATTGTCGCGACCTGTTGTTAAGGACTTCTGTGTCATGCTGGTTGATATTAATCAGTTCAAACGCATCAATGCCC
AATGGGGACATCGTGTGGGTGATAAAGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATGATATT
TTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCAAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACG
TATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACCATCAGTATTGGCA
CGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGC
GAGGGGGGCAACAAAGTGATTATTCATCATATTTAA

Upstream 100 bases:

>100_bases
TAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCA
GAAAACAATCATTCGGAAAA

Downstream 100 bases:

>100_bases
GTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCTTTTGCATACAATTTATTACTATTACCCCTAAATTTCCCCTCCC
CAGCGGCGCAGAGATGAGTA

Product: diguanylate cyclase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MIQSTRISMGLFFKYFLSLTKIDLGQNYISLPSIKSSTHIALLFMVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNENT
NVKLIPQMNYLMVVVALFFLNAVIFLFMLMKYFTNKQILPTLILSLAFLSSLIYLVETIVIIHKPINGSTLIQTKSNDVS
IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYNADYSLYVVDYCPDNHTATWGIN
YTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQE
LQLSSKLAVHDVLTNIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQQSIRPDDI
LARLEGEVFGLLFTKLNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTISIGTVFSTGDTRNISLVMTEADKALREAKS
EGGNKVIIHHI

Sequences:

>Translated_491_residues
MIQSTRISMGLFFKYFLSLTKIDLGQNYISLPSIKSSTHIALLFMVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNENT
NVKLIPQMNYLMVVVALFFLNAVIFLFMLMKYFTNKQILPTLILSLAFLSSLIYLVETIVIIHKPINGSTLIQTKSNDVS
IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYNADYSLYVVDYCPDNHTATWGIN
YTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQE
LQLSSKLAVHDVLTNIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQQSIRPDDI
LARLEGEVFGLLFTKLNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTISIGTVFSTGDTRNISLVMTEADKALREAKS
EGGNKVIIHHI
>Mature_491_residues
MIQSTRISMGLFFKYFLSLTKIDLGQNYISLPSIKSSTHIALLFMVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNENT
NVKLIPQMNYLMVVVALFFLNAVIFLFMLMKYFTNKQILPTLILSLAFLSSLIYLVETIVIIHKPINGSTLIQTKSNDVS
IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYNADYSLYVVDYCPDNHTATWGIN
YTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQE
LQLSSKLAVHDVLTNIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQQSIRPDDI
LARLEGEVFGLLFTKLNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTISIGTVFSTGDTRNISLVMTEADKALREAKS
EGGNKVIIHHI

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1788085, Length=491, Percent_Identity=99.3890020366599, Blast_Score=988, Evalue=0.0,
Organism=Escherichia coli, GI1787262, Length=449, Percent_Identity=37.4164810690423, Blast_Score=296, Evalue=1e-81,
Organism=Escherichia coli, GI87081881, Length=182, Percent_Identity=29.1208791208791, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI87082007, Length=227, Percent_Identity=29.9559471365639, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787802, Length=181, Percent_Identity=30.939226519337, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1786584, Length=234, Percent_Identity=26.9230769230769, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788381, Length=169, Percent_Identity=28.4023668639053, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI145693134, Length=181, Percent_Identity=28.7292817679558, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: NA

Molecular weight: Translated: 56147; Mature: 56147

Theoretical pI: Translated: 9.07; Mature: 9.07

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQSTRISMGLFFKYFLSLTKIDLGQNYISLPSIKSSTHIALLFMVSMGTQKLKAQSFFI
CCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHCHHHHHHHHHHH
FSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNAVIFLFMLMKYFTNKQILP
HHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
TLILSLAFLSSLIYLVETIVIIHKPINGSTLIQTKSNDVSIFYIFRQLSFICLTSLALFC
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEHHHHHHHHHHHHHHHHHH
YGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYNADYSLYVVDYCPDNHTATWGIN
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCEEECCC
YTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTLDEYNYTIWYISRG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECC
IEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLTNIYNRRYFFNSVESLLSRPVVKDFCV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
MLVDINQFKRINAQWGHRVGDKVLVSIVDIIQQSIRPDDILARLEGEVFGLLFTKLNSAQ
HHHHHHHHHHCCCHHCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHCHHH
AKIIAERMRKNVELLTGFSNRYDVPEQMTISIGTVFSTGDTRNISLVMTEADKALREAKS
HHHHHHHHHCCHHHHCCCCCCCCCCCCEEEEEEEEEECCCCCEEEEEEEHHHHHHHHHHH
EGGNKVIIHHI
CCCCEEEEEEC
>Mature Secondary Structure
MIQSTRISMGLFFKYFLSLTKIDLGQNYISLPSIKSSTHIALLFMVSMGTQKLKAQSFFI
CCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHCHHHHHHHHHHH
FSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNAVIFLFMLMKYFTNKQILP
HHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
TLILSLAFLSSLIYLVETIVIIHKPINGSTLIQTKSNDVSIFYIFRQLSFICLTSLALFC
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEHHHHHHHHHHHHHHHHHH
YGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYNADYSLYVVDYCPDNHTATWGIN
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCEEECCC
YTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTLDEYNYTIWYISRG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECC
IEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLTNIYNRRYFFNSVESLLSRPVVKDFCV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
MLVDINQFKRINAQWGHRVGDKVLVSIVDIIQQSIRPDDILARLEGEVFGLLFTKLNSAQ
HHHHHHHHHHCCCHHCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHCHHH
AKIIAERMRKNVELLTGFSNRYDVPEQMTISIGTVFSTGDTRNISLVMTEADKALREAKS
HHHHHHHHHCCHHHHCCCCCCCCCCCCEEEEEEEEEECCCCCEEEEEEEHHHHHHHHHHH
EGGNKVIIHHI
CCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]