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The map label for this gene is gdhA

Identifier: 157161223

GI number: 157161223

Start: 1860453

End: 1861796

Strand: Direct

Name: gdhA

Synonym: EcHS_A1845

Alternate gene names: 157161223

Gene position: 1860453-1861796 (Clockwise)

Preceding gene: 157161221

Following gene: 157161228

Centisome position: 40.07

GC content: 53.2

Gene sequence:

>1344_bases
ATGGATCAGACATATTCTCTGGAGTCATTCCTCAACCATGTCCAAAAGCGCGACCCGAATCAAACCGAGTTCGCGCAAGC
CGTTCGTGAAGTAATGACCACACTCTGGCCTTTTCTTGAACAAAATCCAAAATATCGCCAGATGTCATTACTGGAGCGTC
TGGTTGAACCGGAGCGCGTGATCCAGTTTCGCGTGGTATGGGTTGATGATCGCAACCAGATACAGGTCAACCGTGCATGG
CGTGTGCAGTTCAGCTCTGCCATCGGCCCGTACAAAGGCGGTATGCGCTTCCATCCGTCAGTTAACCTTTCCATTCTCAA
ATTCCTCGGCTTTGAACAAACCTTCAAAAATGCCCTGACTACCCTGCCGATGGGCGGTGGTAAAGGCGGCAGCGATTTCG
ATCCGAAAGGAAAAAGCGAAGGTGAAGTGATGCGTTTTTGCCAGGCGCTGATGACCGAGCTTTATCGTCATCTGGGCGCG
GATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGCTCTC
CAACAATACCGCCTGCGTCTTCACCGGTAAGGGCCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTACCGGCTACG
GTCTGGTTTATTTCACAGAAGCAATGTTAAAACGCCACGGTATGGGTTTTGAAGGGATGCGCGTTTCCGTTTCTGGCTCC
GGCAACGTCGCCCAGTACGCTATCGAAAAAGCGATGGAATTTGGTGCTCGTGTGATCACTGCGTCAGACTCCAGCGGCAC
TGTAGTTGATGAAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGTCGAGTGG
CAGATTACGCCAAAGAATTTGGTCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTACCGGTTGATATCGCCCTGCCT
TGCGCCACCCAGAATGAACTGGATGTTGACGCCGCGCATCAGCTTATCGCTAATGGCGTTAAAGCCGTCGCCGAAGGGGC
AAATATGCCGACCACCATCGAAGCGACTGAACTGTTCCAGCAGGCAGGCGTACTATTTGCACCGGGTAAAGCGGCTAATG
CTGGTGGCGTCGCTACATCGGGCCTGGAAATGGCACAAAACGCTGCGCGCCTGGGCTGGAAAGCCGAGAAAGTTGACGCA
CGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAACCAACTACGTGCA
GGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAA

Upstream 100 bases:

>100_bases
GCTTACCGTCACATTCTTGATGGTATAGTCGAAAACTGCAAAAGCACATGACATAAACAACATAAGCACAATCGTATTAA
TATATAAGGGTTTTATATCT

Downstream 100 bases:

>100_bases
GTTGTAAATGCCTGATGGCGCTACGCTTATCAGGCCTACAAATGGGCACAATTCATTGCAGTTACGCTCTAATGTAGGCC
GGGCAAGCGCAGCGCCCCCG

Product: glutamate dehydrogenase

Products: NA

Alternate protein names: NADP-GDH

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPKYRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAW
RVQFSSAIGPYKGGMRFHPSVNLSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYFTEAMLKRHGMGFEGMRVSVSGS
GNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTKEKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSLPVDIALP
CATQNELDVDAAHQLIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLGWKAEKVDA
RLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI

Sequences:

>Translated_447_residues
MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPKYRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAW
RVQFSSAIGPYKGGMRFHPSVNLSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYFTEAMLKRHGMGFEGMRVSVSGS
GNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTKEKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSLPVDIALP
CATQNELDVDAAHQLIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLGWKAEKVDA
RLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI
>Mature_447_residues
MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPKYRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAW
RVQFSSAIGPYKGGMRFHPSVNLSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYFTEAMLKRHGMGFEGMRVSVSGS
GNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTKEKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSLPVDIALP
CATQNELDVDAAHQLIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLGWKAEKVDA
RLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family

Homologues:

Organism=Homo sapiens, GI31377775, Length=406, Percent_Identity=30.0492610837438, Blast_Score=135, Evalue=8e-32,
Organism=Homo sapiens, GI4885281, Length=390, Percent_Identity=29.7435897435897, Blast_Score=131, Evalue=2e-30,
Organism=Escherichia coli, GI1788059, Length=447, Percent_Identity=100, Blast_Score=926, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17544676, Length=345, Percent_Identity=29.2753623188406, Blast_Score=114, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6324951, Length=452, Percent_Identity=50.8849557522124, Blast_Score=422, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6319256, Length=454, Percent_Identity=51.9823788546256, Blast_Score=419, Evalue=1e-118,
Organism=Drosophila melanogaster, GI24649283, Length=318, Percent_Identity=31.4465408805031, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI45549226, Length=382, Percent_Identity=29.0575916230367, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI45553475, Length=382, Percent_Identity=29.0575916230367, Blast_Score=122, Evalue=6e-28,
Organism=Drosophila melanogaster, GI24649460, Length=306, Percent_Identity=30.3921568627451, Blast_Score=101, Evalue=8e-22,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): DHE4_ECOLI (P00370)

Other databases:

- EMBL:   J01615
- EMBL:   K02499
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   A00382
- RefSeq:   AP_002380.1
- RefSeq:   NP_416275.1
- ProteinModelPortal:   P00370
- SMR:   P00370
- DIP:   DIP-9756N
- STRING:   P00370
- SWISS-2DPAGE:   P00370
- EnsemblBacteria:   EBESCT00000003963
- EnsemblBacteria:   EBESCT00000018103
- GeneID:   946802
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW1750
- KEGG:   eco:b1761
- EchoBASE:   EB0367
- EcoGene:   EG10372
- eggNOG:   COG0334
- GeneTree:   EBGT00050000010609
- HOGENOM:   HBG590661
- OMA:   KGLTYGG
- ProtClustDB:   PRK09414
- BioCyc:   EcoCyc:GDHA-MONOMER
- BioCyc:   MetaCyc:GDHA-MONOMER
- Genevestigator:   P00370
- GO:   GO:0005737
- GO:   GO:0005488
- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR11606:SF2
- PIRSF:   PIRSF000185
- PRINTS:   PR00082
- SMART:   SM00839

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N

EC number: =1.4.1.4

Molecular weight: Translated: 48582; Mature: 48582

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: ACT_SITE 128-128

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPKYRQMSLLERLVEPERV
CCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCE
IQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVNLSILKFLGFEQTFKNALT
EEEEEEEECCCCEEEEEEEEEEEECCCCCCCCCCEEECCCCCHHHHHHHCHHHHHHHHHH
TLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGADTDVPAGDIGVGGREVGFMA
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHH
GMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYFTEAMLKRHGMGFEGMRVSVSGS
HHHHHHCCCCEEEEECCCCCCCCCEECCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCC
GNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTKEKLARLIEIKASRDGRVADYAKEF
CCHHHHHHHHHHHCCCEEEEECCCCCCEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHC
GLVYLEGQQPWSLPVDIALPCATQNELDVDAAHQLIANGVKAVAEGANMPTTIEATELFQ
CEEEEECCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
QAGVLFAPGKAANAGGVATSGLEMAQNAARLGWKAEKVDARLHHIMLDIHHACVEHGGEG
HCCCEECCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
EQTNYVQGANIAGFVKVADAMLAQGVI
CCCCEECCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPKYRQMSLLERLVEPERV
CCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCE
IQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVNLSILKFLGFEQTFKNALT
EEEEEEEECCCCEEEEEEEEEEEECCCCCCCCCCEEECCCCCHHHHHHHCHHHHHHHHHH
TLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGADTDVPAGDIGVGGREVGFMA
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHH
GMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYFTEAMLKRHGMGFEGMRVSVSGS
HHHHHHCCCCEEEEECCCCCCCCCEECCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCC
GNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTKEKLARLIEIKASRDGRVADYAKEF
CCHHHHHHHHHHHCCCEEEEECCCCCCEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHC
GLVYLEGQQPWSLPVDIALPCATQNELDVDAAHQLIANGVKAVAEGANMPTTIEATELFQ
CEEEEECCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
QAGVLFAPGKAANAGGVATSGLEMAQNAARLGWKAEKVDARLHHIMLDIHHACVEHGGEG
HCCCEECCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
EQTNYVQGANIAGFVKVADAMLAQGVI
CCCCEECCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6308576; 6373501; 9097039; 9278503; 9298646