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The map label for this gene is nemA

Identifier: 157161113

GI number: 157161113

Start: 1748610

End: 1749707

Strand: Direct

Name: nemA

Synonym: EcHS_A1727

Alternate gene names: 157161113

Gene position: 1748610-1749707 (Clockwise)

Preceding gene: 157161112

Following gene: 157161114

Centisome position: 37.66

GC content: 53.55

Gene sequence:

>1098_bases
ATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCGTATTTTTATGGCACCGCTGAC
GCGTCTGCGCAGTATTGAACCGGGTGACATTCCTACCCCGTTGATGGCGGAATACTATCGCCAACGTGCCAGTGCCGGTT
TGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCCTGGCATCCATAGTCCGGAGCAA
ATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACG
CATTTCTCACGCCAGCCTGCAACCTGGCGGTCAGGCACCGGTAGCGCCTTCAGCACTTAGCGCGGGAACACGTACTTCTC
TGCGCGATGAAAATGGTCAGGCGATCCGTGTTGAAACATCCATGCCGCGTGCGCTTGAACTGGAAGAGATTCCAGGTATC
GTCAATGATTTCCGTCAGGCCATTGCTAACGCGCGTGAAGCCGGTTTTGATCTGGTAGAGCTCCACTCTGCTCACGGTTA
TTTGCTGCATCAGTTCCTTTCTCCTTCTTCAAACCATCGTACCGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTT
TGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCGCCAATCGGTACT
TTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGC
TTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTT
TCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGGTTAATTGATGCG
GTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCG
TGCCGAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATACCGATTACCCGACGTTGTAA

Upstream 100 bases:

>100_bases
AGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTCA
CCAGACGACCGGGAGCCTTT

Downstream 100 bases:

>100_bases
TCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAG
ATGCGTCTTCTTCATACCAT

Product: N-ethylmaleimide reductase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAEYYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQ
IAAWKKITAGVHAENGHMAVQLWHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEVVDAGIEEWGADRIGIRVSPIGT
FQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDWAGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDA
VAFGRDWIANPDLVARLQRKAELNPQRAESFYGGGAEGYTDYPTL

Sequences:

>Translated_365_residues
MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAEYYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQ
IAAWKKITAGVHAENGHMAVQLWHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEVVDAGIEEWGADRIGIRVSPIGT
FQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDWAGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDA
VAFGRDWIANPDLVARLQRKAELNPQRAESFYGGGAEGYTDYPTL
>Mature_364_residues
SSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAEYYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQI
AAWKKITAGVHAENGHMAVQLWHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGIV
NDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEVVDAGIEEWGADRIGIRVSPIGTF
QNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDWAGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAV
AFGRDWIANPDLVARLQRKAELNPQRAESFYGGGAEGYTDYPTL

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family

Homologues:

Organism=Escherichia coli, GI1787939, Length=365, Percent_Identity=100, Blast_Score=743, Evalue=0.0,
Organism=Escherichia coli, GI1789463, Length=359, Percent_Identity=31.4763231197772, Blast_Score=120, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17565138, Length=356, Percent_Identity=30.3370786516854, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17559802, Length=350, Percent_Identity=28.5714285714286, Blast_Score=104, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI17559804, Length=268, Percent_Identity=29.8507462686567, Blast_Score=95, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17564188, Length=351, Percent_Identity=27.0655270655271, Blast_Score=94, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17540738, Length=374, Percent_Identity=28.6096256684492, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI72001454, Length=354, Percent_Identity=30.225988700565, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17566914, Length=216, Percent_Identity=30.5555555555556, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6325086, Length=389, Percent_Identity=39.0745501285347, Blast_Score=236, Evalue=3e-63,
Organism=Saccharomyces cerevisiae, GI6321973, Length=389, Percent_Identity=37.2750642673522, Blast_Score=219, Evalue=6e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NEMA_ECOLI (P77258)

Other databases:

- EMBL:   D86931
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   JC5605
- RefSeq:   AP_002272.1
- RefSeq:   NP_416167.1
- ProteinModelPortal:   P77258
- SMR:   P77258
- STRING:   P77258
- SWISS-2DPAGE:   P77258
- EnsemblBacteria:   EBESCT00000000677
- EnsemblBacteria:   EBESCT00000016815
- GeneID:   946164
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW1642
- KEGG:   eco:b1650
- EchoBASE:   EB3316
- EcoGene:   EG13546
- eggNOG:   COG1902
- GeneTree:   EBGT00050000011021
- HOGENOM:   HBG583461
- OMA:   SANQRED
- ProtClustDB:   PRK10605
- BioCyc:   EcoCyc:G6890-MONOMER
- BioCyc:   MetaCyc:G6890-MONOMER
- Genevestigator:   P77258
- InterPro:   IPR013785
- InterPro:   IPR001155
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF00724 Oxidored_FMN

EC number: 1.-.-.- [C]

Molecular weight: Translated: 39517; Mature: 39386

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: NA

Important sites: ACT_SITE 187-187 BINDING 101-101 BINDING 234-234 BINDING 325-325

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAEYYRQRASAGLIISEAT
CCCCHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEEHHH
QISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQLWHTGRISHASLQPGGQAP
HHHHHCCCCCCCCCCCCHHHHHHHHHHHCCEEECCCCEEEEEEECCCCCCCCCCCCCCCC
VAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGIVNDFRQAIANAREAGFDLVE
CCCHHHCCCCCCCCCCCCCCEEEEECCCCCEECHHHCCCHHHHHHHHHHHHHHCCCCEEE
LHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEVVDAGIEEWGADRIGIRVSPIGT
HHCCCHHHHHHHHCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCC
FQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDWAGGEPYTDAFREKVRARFHGPIIG
CCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEE
AGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLVARLQRKAELNPQRAESFYGGGAEGYT
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCC
DYPTL
CCCCC
>Mature Secondary Structure 
SSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAEYYRQRASAGLIISEAT
CCCHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEEHHH
QISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQLWHTGRISHASLQPGGQAP
HHHHHCCCCCCCCCCCCHHHHHHHHHHHCCEEECCCCEEEEEEECCCCCCCCCCCCCCCC
VAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGIVNDFRQAIANAREAGFDLVE
CCCHHHCCCCCCCCCCCCCCEEEEECCCCCEECHHHCCCHHHHHHHHHHHHHHCCCCEEE
LHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEVVDAGIEEWGADRIGIRVSPIGT
HHCCCHHHHHHHHCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCC
FQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDWAGGEPYTDAFREKVRARFHGPIIG
CCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEE
AGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLVARLQRKAELNPQRAESFYGGGAEGYT
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCC
DYPTL
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503