Click here to switch to the map view.

The map label for this gene is yneJ [H]

Identifier: 157160998

GI number: 157160998

Start: 1631626

End: 1632507

Strand: Direct

Name: yneJ [H]

Synonym: EcHS_A1608

Alternate gene names: 157160998

Gene position: 1631626-1632507 (Clockwise)

Preceding gene: 157160991

Following gene: 157160999

Centisome position: 35.14

GC content: 53.85

Gene sequence:

>882_bases
ATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCG
CGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCTGGAAACAGAACTGGGGGTTGATCTGTTTATTCGCGAGAATCAGC
GTTTACGTCTCTCTCCTGCCGGGCATAACTTTTTACGCTATAGCCAACAAATTCTCGCGTTAGTGGATGAAGCGCGGAGC
GTTGTCGCTGGCGATGAGCCGCAAGGTTTATTTTCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCAGCCAC
GCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCACTATGCTGGATGGTG
TACTGGAGGGAAAACTGAATGCGGCGTTTATTGATGGACCCATTAACCATACTGCCATCGACGGGATACCGGTATACCGC
GAGGAACTGATGATCGTCACGCCACAAGGATATGCGCCAGTAACCCGTGCCAGTCAGGTTAATGGCAGTAACATTTATGC
CTTCCGCGCCAATTGTTCGTATCGTCGCCACTTCGAGAGCTGGTTTCATGCTGACGGTGCCGCTCCGGGAACTATCCATG
AGATGGAGTCTTATCACGGAATGTTGGCCTGTGTGATCGCAGGAGCAGGCATTGCGCTTATTCCGCGCTCTATGCTGGAA
AGTATGCCGGGGCATCACCAGGTTGAAGCGTGGCCGTTAGCTGAGCAATGGCGTTGGTTAACAACCTGGCTGGTCTGGCG
TCGTGGTGCGAAAACGCGCCCGCTCGAGGCATTTATTCAACTGCTGGAGGTGCCTGACTCGGCAAGACAGGGATATCAAT
GA

Upstream 100 bases:

>100_bases
CGGGGTATCTCCTTTATGAGTCATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTT
ATTCACGAATCGAGAATGCT

Downstream 100 bases:

>100_bases
GCTATTTTTGATAGTTCTTGCGGCTAATATGCTCTATATAGTGATGTTCCGATGACTTATGACTATATGGGGCAAATATG
GTTACGCCAGTAAGCATCAG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELGVDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARS
VVAGDEPQGLFSLGSLESTAAVRIPATLAEFNRRYPKIQFSLSTGPSGTMLDGVLEGKLNAAFIDGPINHTAIDGIPVYR
EELMIVTPQGYAPVTRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESYHGMLACVIAGAGIALIPRSMLE
SMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLEAFIQLLEVPDSARQGYQ

Sequences:

>Translated_293_residues
MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELGVDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARS
VVAGDEPQGLFSLGSLESTAAVRIPATLAEFNRRYPKIQFSLSTGPSGTMLDGVLEGKLNAAFIDGPINHTAIDGIPVYR
EELMIVTPQGYAPVTRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESYHGMLACVIAGAGIALIPRSMLE
SMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLEAFIQLLEVPDSARQGYQ
>Mature_293_residues
MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELGVDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARS
VVAGDEPQGLFSLGSLESTAAVRIPATLAEFNRRYPKIQFSLSTGPSGTMLDGVLEGKLNAAFIDGPINHTAIDGIPVYR
EELMIVTPQGYAPVTRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESYHGMLACVIAGAGIALIPRSMLE
SMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLEAFIQLLEVPDSARQGYQ

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787806, Length=293, Percent_Identity=99.6587030716723, Blast_Score=603, Evalue=1e-174,
Organism=Escherichia coli, GI1788887, Length=294, Percent_Identity=25.5102040816327, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI87082132, Length=287, Percent_Identity=24.390243902439, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI157672245, Length=285, Percent_Identity=24.5614035087719, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1790208, Length=256, Percent_Identity=24.609375, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1790262, Length=290, Percent_Identity=23.448275862069, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1788481, Length=180, Percent_Identity=31.1111111111111, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787879, Length=285, Percent_Identity=23.859649122807, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI145693105, Length=288, Percent_Identity=21.1805555555556, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1788297, Length=258, Percent_Identity=24.4186046511628, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1790399, Length=289, Percent_Identity=21.7993079584775, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788706, Length=176, Percent_Identity=27.8409090909091, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1786401, Length=169, Percent_Identity=25.4437869822485, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32497; Mature: 32497

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELGVDLFIRENQRLRLSPA
CCCHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEEEECCCEEEECCC
GHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAVRIPATLAEFNRRYPKIQF
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCEEEECCHHHHHHHCCCCEEEE
SLSTGPSGTMLDGVLEGKLNAAFIDGPINHTAIDGIPVYREELMIVTPQGYAPVTRASQV
EECCCCCCHHHHHHHHCCCCEEEECCCCCCCCCCCCCEECCCEEEECCCCCCCCCCCCCC
NGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESYHGMLACVIAGAGIALIPRSMLE
CCCEEEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
SMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLEAFIQLLEVPDSARQGYQ
HCCCCCCEEECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHCCCC
>Mature Secondary Structure
MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELGVDLFIRENQRLRLSPA
CCCHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEEEECCCEEEECCC
GHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAVRIPATLAEFNRRYPKIQF
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCEEEECCHHHHHHHCCCCEEEE
SLSTGPSGTMLDGVLEGKLNAAFIDGPINHTAIDGIPVYREELMIVTPQGYAPVTRASQV
EECCCCCCHHHHHHHHCCCCEEEECCCCCCCCCCCCCEECCCEEEECCCCCCCCCCCCCC
NGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESYHGMLACVIAGAGIALIPRSMLE
CCCEEEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
SMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLEAFIQLLEVPDSARQGYQ
HCCCCCCEEECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]