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The map label for this gene is glsA2

Identifier: 157160996

GI number: 157160996

Start: 1629147

End: 1630073

Strand: Reverse

Name: glsA2

Synonym: EcHS_A1606

Alternate gene names: 157160996

Gene position: 1630073-1629147 (Counterclockwise)

Preceding gene: 157160997

Following gene: 157160995

Centisome position: 35.1

GC content: 52.0

Gene sequence:

>927_bases
GTGGCAGTCGCCATGGATAATGCAATTTTAGAAAACATCTTGCGGCAAGTGCGGCCGCTCATTGGTCAGGGTAAAGTCGC
GGATTATATTCCGGCGCTGGCTACAGTAGACGGTTCCCGATTGGGGATTGCTATCTGTACCGTTGACGGACAGCTTTTTC
AGGCCGGAGACGCGCAAGAACGTTTTTCCATTCAGTCTATTTCCAAAGTGCTGAGTCTCGTTGTCGCCATGCGTCATTAC
TCCGAAGAGGAAATCTGGCAACGCGTCGGCAAAGATCCGTCTGGATCACCGTTCAATTCCTTAGTGCAACTGGAAATGGA
GCAGGGTATACCGCGTAATCCGTTCATTAATGCCGGTGCGCTGGTGGTCTGCGATATGTTGCAAGGGCGATTAAGCGCAC
CACGGCAACGTATGCTGGAAGTCGTGCGCGGCTTAAGCGGTGTGTCTGATATTTCCTACGATACGGTGGTAGCGCGTTCC
GAATTTGAACATTCCGCGCGAAATGCGGCTATCGCCTGGCTGATGAAGTCGTTTGGCAATTTCCATCATGACGTGACAAC
CGTTCTGCAAAACTACTTTCATTACTGCGCTCTGAAAATGAGCTGTGTAGAGCTGGCCCGGACGTTTGTCTTTCTGGCTA
ATCAGGGGAAAGCTATTCATATTGATGAACCAGTGGTGACGCCAATGCAGGCGCGGCAAATTAACGCGCTGATGGCGACC
AGTGGTATGTACCAGAACGCGGGGGAGTTTGCCTGGCGGGTGGGACTACCGGCGAAATCTGGCGTTGGGGGCGGTATTGT
GGCGATTGTTCCGCATGAAATGGCCATCGCAGTCTGGAGTCCGGAACTGGATGATGCAGGTAACTCGCTTGCGGGTATCG
CCGTTCTTGAACAATTAACGAAACAGTTAGGGCGTTCGGTTTATTAA

Upstream 100 bases:

>100_bases
CTGTAATATCCAGACGGTGTGGAAAGACCGGATCTGACGCGACATATTCAGCTCTGATATACTCGCAGGTCTTTTCAGAC
CTGCGGTCCAGGAGTAGAAA

Downstream 100 bases:

>100_bases
TGCAGTCTCTCGATCCACTCTTCGCGCGTTTATCCCGTTCAAAATTTCGCTCTCGCTTTCGTTTGGGCATGAAAGAGCGT
CAGTATTGTCTGGAGAAAGG

Product: glutaminase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIAICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHY
SEEEIWQRVGKDPSGSPFNSLVQLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKAIHIDEPVVTPMQARQINALMAT
SGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAIAVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY

Sequences:

>Translated_308_residues
MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIAICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHY
SEEEIWQRVGKDPSGSPFNSLVQLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKAIHIDEPVVTPMQARQINALMAT
SGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAIAVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY
>Mature_307_residues
AVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIAICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYS
EEEIWQRVGKDPSGSPFNSLVQLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARSE
FEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKAIHIDEPVVTPMQARQINALMATS
GMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAIAVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY

Specific function: Unknown

COG id: COG2066

COG function: function code E; Glutaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutaminase family

Homologues:

Organism=Homo sapiens, GI156104878, Length=280, Percent_Identity=37.8571428571429, Blast_Score=185, Evalue=4e-47,
Organism=Homo sapiens, GI20336214, Length=294, Percent_Identity=34.0136054421769, Blast_Score=165, Evalue=4e-41,
Organism=Escherichia coli, GI1787804, Length=308, Percent_Identity=100, Blast_Score=635, Evalue=0.0,
Organism=Escherichia coli, GI1786693, Length=286, Percent_Identity=38.4615384615385, Blast_Score=197, Evalue=7e-52,
Organism=Caenorhabditis elegans, GI193204073, Length=309, Percent_Identity=35.9223300970874, Blast_Score=181, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI193204075, Length=311, Percent_Identity=35.6913183279743, Blast_Score=181, Evalue=5e-46,
Organism=Caenorhabditis elegans, GI17507019, Length=291, Percent_Identity=36.426116838488, Blast_Score=179, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI17532727, Length=304, Percent_Identity=33.2236842105263, Blast_Score=172, Evalue=1e-43,
Organism=Drosophila melanogaster, GI281363241, Length=294, Percent_Identity=35.3741496598639, Blast_Score=187, Evalue=1e-47,
Organism=Drosophila melanogaster, GI24653164, Length=294, Percent_Identity=35.3741496598639, Blast_Score=187, Evalue=1e-47,
Organism=Drosophila melanogaster, GI24653158, Length=294, Percent_Identity=35.3741496598639, Blast_Score=186, Evalue=1e-47,
Organism=Drosophila melanogaster, GI24653162, Length=294, Percent_Identity=35.3741496598639, Blast_Score=186, Evalue=1e-47,
Organism=Drosophila melanogaster, GI281363239, Length=294, Percent_Identity=35.3741496598639, Blast_Score=186, Evalue=1e-47,
Organism=Drosophila melanogaster, GI24653156, Length=294, Percent_Identity=35.3741496598639, Blast_Score=186, Evalue=1e-47,
Organism=Drosophila melanogaster, GI24653166, Length=294, Percent_Identity=35.3741496598639, Blast_Score=186, Evalue=2e-47,
Organism=Drosophila melanogaster, GI116008307, Length=285, Percent_Identity=36.140350877193, Blast_Score=186, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLSA2_ECO57 (P0A6W2)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   C90895
- PIR:   E85722
- RefSeq:   NP_287629.1
- RefSeq:   NP_310158.1
- ProteinModelPortal:   P0A6W2
- SMR:   P0A6W2
- EnsemblBacteria:   EBESCT00000027588
- EnsemblBacteria:   EBESCT00000058272
- GeneID:   917332
- GeneID:   960641
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z2179
- KEGG:   ecs:ECs2131
- GeneTree:   EBGT00050000011240
- HOGENOM:   HBG512335
- OMA:   RNASIAY
- ProtClustDB:   PRK00971
- BioCyc:   ECOL83334:ECS2131-MONOMER
- HAMAP:   MF_00313
- InterPro:   IPR012338
- InterPro:   IPR015868
- Gene3D:   G3DSA:3.40.710.20
- PANTHER:   PTHR12544
- TIGRFAMs:   TIGR03814

Pfam domain/function: PF04960 Glutaminase; SSF56601 PBP_transp_fold

EC number: =3.5.1.2

Molecular weight: Translated: 33516; Mature: 33385

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIAICTVDGQLFQAGDAQE
CCEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEEEEECCHHHCCCCHHH
RFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLVQLEMEQGIPRNPFINAGA
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCH
LVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARSEFEHSARNAAIAWLMKSFGN
HHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
FHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKAIHIDEPVVTPMQARQINALMAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHH
SGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAIAVWSPELDDAGNSLAGIAVLEQLT
CCCCCCCCCEEEEECCCCCCCCCCCEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHH
KQLGRSVY
HHHCCCCC
>Mature Secondary Structure 
AVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIAICTVDGQLFQAGDAQE
CEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEEEEECCHHHCCCCHHH
RFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLVQLEMEQGIPRNPFINAGA
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCH
LVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARSEFEHSARNAAIAWLMKSFGN
HHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
FHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKAIHIDEPVVTPMQARQINALMAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHH
SGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAIAVWSPELDDAGNSLAGIAVLEQLT
CCCCCCCCCEEEEECCCCCCCCCCCEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHH
KQLGRSVY
HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796