Click here to switch to the map view.

The map label for this gene is lsrF

Identifier: 157160989

GI number: 157160989

Start: 1622922

End: 1623797

Strand: Direct

Name: lsrF

Synonym: EcHS_A1599

Alternate gene names: 157160989

Gene position: 1622922-1623797 (Clockwise)

Preceding gene: 157160988

Following gene: 157160990

Centisome position: 34.95

GC content: 49.43

Gene sequence:

>876_bases
ATGGCAGATTTAGACGATATTAAAGATGGTAAAGATTTTCGTACCGATCAACCGCAACAAAATATCCCTTTTACCCTGAA
AGGTTGCGGTGCGCTGGATTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAATACCGTGATGC
TGGCCTTTGATCATGGTTATTTTCAGGGGCCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAA
CATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCAGCGACCAATAAGCCGGTGGTACTGAG
GGCGTCAGGTGCGAACTCTATTCTGGCGGAATTAAGTAATGAAGCCGTGGCGTTATCGATGGATGATGCCGTGCGCCTGA
ACAGTTGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAAAATATTATTCAGCTGGTT
GATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTGGGCAAAGATATGGTGCGCGATCAGCGTTATTT
CTCGCTCGCGACTCGAATCGCCGCTGAAATGGGGGCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGA
TTGTTGCCGGATGTCCGGTACCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGG
CAGGCTATCGATCAGGGCGCTTCTGGTGTGGATATGGGGCGTAATATTTTCCAGTCTGACCATCCGGTGGCGATGATGAA
AGCCGTACAGGCGGTGGTTCACCATAACGAAACGGCTGATCGAGCATATGAACTCTATCTGAGTGAAAAACAGTAA

Upstream 100 bases:

>100_bases
ATGGTAATGGCATCGTACTGTTACCGGAGCGCGTGATATTCAACAAAGAGAATATCGGCAAATACGATTTCTGATGTGCA
TTACTTAACCGGAGTAAGTT

Downstream 100 bases:

>100_bases
CTGCGGATCTAAGGAGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACAAGGTTGACGAGTTTATC
GAAGTTTTTCGCCAGAACCA

Product: aldolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSRIFNPKTGNTVMLAFDHGYFQGPTTGLERIDINIAPLFE
HADVLMCTRGILRSVVPPATNKPVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGCPVPIVIAGGKKLPEREALEMCW
QAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHNETADRAYELYLSEKQ

Sequences:

>Translated_291_residues
MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSRIFNPKTGNTVMLAFDHGYFQGPTTGLERIDINIAPLFE
HADVLMCTRGILRSVVPPATNKPVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGCPVPIVIAGGKKLPEREALEMCW
QAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHNETADRAYELYLSEKQ
>Mature_290_residues
ADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSRIFNPKTGNTVMLAFDHGYFQGPTTGLERIDINIAPLFEH
ADVLMCTRGILRSVVPPATNKPVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLVD
AGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGCPVPIVIAGGKKLPEREALEMCWQ
AIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHNETADRAYELYLSEKQ

Specific function: Could be involved in the degradation of phospho-AI-2, thereby terminating induction of the lsr operon and closing the AI-2 signaling cycle

COG id: COG1830

COG function: function code G; DhnA-type fructose-1,6-bisphosphate aldolase and related enzymes

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the deoC/fbaB aldolase family

Homologues:

Organism=Escherichia coli, GI1787796, Length=291, Percent_Identity=98.9690721649485, Blast_Score=603, Evalue=1e-174,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LSRF_ECO24 (A7ZLX4)

Other databases:

- EMBL:   CP000800
- RefSeq:   YP_001462803.1
- ProteinModelPortal:   A7ZLX4
- SMR:   A7ZLX4
- STRING:   A7ZLX4
- EnsemblBacteria:   EBESCT00000022343
- GeneID:   5587401
- GenomeReviews:   CP000800_GR
- KEGG:   ecw:EcE24377A_1717
- eggNOG:   COG1830
- GeneTree:   EBGT00050000010813
- HOGENOM:   HBG553580
- OMA:   FQSEAPV
- ProtClustDB:   PRK08227
- BioCyc:   ECOL331111:ECE24377A_1717-MONOMER
- GO:   GO:0005737
- InterPro:   IPR013785
- InterPro:   IPR002915
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF01791 DeoC

EC number: 4.2.1.- [C]

Molecular weight: Translated: 31851; Mature: 31720

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: NA

Important sites: ACT_SITE 203-203

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSRIFNPKTGNTVMLAFDHGY
CCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCC
FQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNKPVVLRASGANSILAELSN
CCCCCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHCC
EAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLVDAGMKVGMPTMAVTGVGKDM
CEEEEECCCCHHHHHHHEEEHEEECCCCCHHHHHHHHHHHHHHHHCCCCHHEEECCCHHH
VRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGCPVPIVIAGGKKLPEREALEMCW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHH
QAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHNETADRAYELYLSEKQ
HHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHEEEEEECCC
>Mature Secondary Structure 
ADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSRIFNPKTGNTVMLAFDHGY
CCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCC
FQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNKPVVLRASGANSILAELSN
CCCCCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHCC
EAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLVDAGMKVGMPTMAVTGVGKDM
CEEEEECCCCHHHHHHHEEEHEEECCCCCHHHHHHHHHHHHHHHHCCCCHHEEECCCHHH
VRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGCPVPIVIAGGKKLPEREALEMCW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHH
QAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHNETADRAYELYLSEKQ
HHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA