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The map label for this gene is narI1

Identifier: 157160941

GI number: 157160941

Start: 1552749

End: 1553429

Strand: Reverse

Name: narI1

Synonym: EcHS_A1549

Alternate gene names: 157160941

Gene position: 1553429-1552749 (Counterclockwise)

Preceding gene: 157160942

Following gene: 157160940

Centisome position: 33.45

GC content: 52.72

Gene sequence:

>681_bases
ATGATTCAGTATCTGAACGTCTTTTTTTACGATATCTACCCGTACATTTGTGCGACGGTGTTTTTCCTCGGCAGCTGGCT
GCGCTACGACTACGGGCAGTACACCTGGCGCGCCTCCTCAAGTCAAATGCTCGATAAACGCGGGATGGTGATATGGTCGA
ATCTGTTCCATATCGGCATTTTGGGGATTTTCTTCGGGCACTTGTTCGGCATGTTAACGCCGCACTGGATGTACGCGTGG
TTTTTGCCAGTGGCAGCGAAACAACTCATGGCGATGGTGCTCGGCGGTATTTGCGGTGTGTTGACGTTGATTGGCGGCGC
AGGACTGCTGTGGCGCAGGCTGACAAATCAGCGCGTGCGTGCCACTTCCACTACGCCGGATATCATCATCATGAGCATTT
TGCTGATCCAGTGTCTGTTGGGGCTAAGTACCATACCGTTTTCAGCACAGTATCCTGATGGTAGCGAAATGATGAAGCTG
GTGGGTTGGGCGCAAAGCATTGTCACTTTCCGTGGTGGATCGTCAGAGATGCTCAACGGCGTAGCGTTCGTCTTCCGCCT
GCATCTGGTGTTGGGAATGACCATCTTCCTGCTCTTCCCGTTCACCCGACTGGTGCATGTGTGGAGCGCGCCGTTTGAGT
ACTTTACTCGTCGATATCAAATTGTGCGTTCGCGGCGATAA

Upstream 100 bases:

>100_bases
ACGCCACGGCGTGTGACAGCTCGCCGCTTAATCAATATCAGCGACGCTTTAGCCAGGATGTCGCGCCGCAATATGTCGAC
ATCAGTGCGGGAGGTGGGAA

Downstream 100 bases:

>100_bases
TTTCACCCTGGCCGGATAGCCCTTCGCTCTCCGGCCACTTGCAGGCTATGATAGTTCTCCAGTCACAGGAGAACGTTAAT
GAAACCGCAGGTGTACCACG

Product: respiratory nitrate reductase, gamma subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQMLDKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAW
FLPVAAKQLMAMVLGGICGVLTLIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRRYQIVRSRR

Sequences:

>Translated_226_residues
MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQMLDKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAW
FLPVAAKQLMAMVLGGICGVLTLIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRRYQIVRSRR
>Mature_226_residues
MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQMLDKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAW
FLPVAAKQLMAMVLGGICGVLTLIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRRYQIVRSRR

Specific function: This is a second nitrate reductase enzyme which can substitute for the NRA enzyme and allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The gamma chain is a membrane-embedded heme-iron unit resembling cytochrome b, which transf

COG id: COG2181

COG function: function code C; Nitrate reductase gamma subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787738, Length=226, Percent_Identity=100, Blast_Score=459, Evalue=1e-131,
Organism=Escherichia coli, GI1787480, Length=225, Percent_Identity=71.5555555555555, Blast_Score=340, Evalue=6e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NARV_ECO57 (P0AF33)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   D90887
- PIR:   E85730
- RefSeq:   NP_287691.1
- RefSeq:   NP_310095.1
- ProteinModelPortal:   P0AF33
- SMR:   P0AF33
- EnsemblBacteria:   EBESCT00000024256
- EnsemblBacteria:   EBESCT00000056573
- GeneID:   917268
- GeneID:   960731
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z2247
- KEGG:   ecs:ECs2068
- GeneTree:   EBGT00050000011191
- HOGENOM:   HBG308661
- OMA:   DILILCI
- ProtClustDB:   CLSK880007
- BioCyc:   ECOL83334:ECS2068-MONOMER
- GO:   GO:0006810
- InterPro:   IPR003816
- TIGRFAMs:   TIGR00351

Pfam domain/function: PF02665 Nitrate_red_gam

EC number: =1.7.99.4

Molecular weight: Translated: 26018; Mature: 26018

Theoretical pI: Translated: 10.11; Mature: 10.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x14509698)-; HASH(0x14ac3ed0)-; HASH(0x148094a4)-; HASH(0x14470ba0)-; HASH(0x14461db0)-;

Cys/Met content:

1.3 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQMLDKRGMVIWSNLFHIGI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHCCHHHHHHHHHHHH
LGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLTLIGGAGLLWRRLTNQRVR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHC
ATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKLVGWAQSIVTFRGGSSEMLNG
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHH
VAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRRYQIVRSRR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCH
>Mature Secondary Structure
MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQMLDKRGMVIWSNLFHIGI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHCCHHHHHHHHHHHH
LGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLTLIGGAGLLWRRLTNQRVR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHC
ATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKLVGWAQSIVTFRGGSSEMLNG
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHH
VAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRRYQIVRSRR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796