Click here to switch to the map view.

The map label for this gene is ogt [H]

Identifier: 157160846

GI number: 157160846

Start: 1445973

End: 1446488

Strand: Reverse

Name: ogt [H]

Synonym: EcHS_A1451

Alternate gene names: 157160846

Gene position: 1446488-1445973 (Counterclockwise)

Preceding gene: 157160847

Following gene: 157160845

Centisome position: 31.15

GC content: 52.91

Gene sequence:

>516_bases
ATGCTGAGATTACTTGAAGAAAAAATTGCCACGCCACTGGGTCCACTGTGGGTGATTTGCGATGAACAATTTCGTCTGCG
GGCGGTTGAATGGGAAGAGTACAGCGAACGCATGGTGCAGCTGCTGGACATCCATTATCGCAAAGAAGGCTATGAGCGCA
TTTCTGCCACCAACCCAGGCGGTTTAAGCGACAAGCTTCGTGATTATTTTGCCGGTAATCTTAGCATTATTGATACGCTT
CCCACTGCCACAGGGGGGACGCCATTTCAGCGCGAAGTCTGGAAAACACTACGCACTATCCCCTGCGGGCAGGTAATGCA
TTACGGCCAACTGGCTGAACAATTGGGCCGCCCTGGCGCGGCGCGTGCCGTTGGTGCGGCAAACGGATCGAATCCCATCA
GCATCGTCGTACCTTGCCATCGGGTTATTGGCCGAAACGGCACCATGACCGGATATGCAGGCGGAGTTCAGCGAAAAGAG
TGGTTATTGCGCCATGAAGGTTATCTTTTGCTGTAA

Upstream 100 bases:

>100_bases
ATCTTTTTGGTGGTATGGCTGCTGATGTTGCTGGCGTGGTATCTTGTTGGTCTGCCGATAGGTCCGGGGATTTCCCCACG
TTTGTCTTAAGAGAGAACGG

Downstream 100 bases:

>100_bases
ACATTAAACAATTTGTGCCAGCTTGTTCACACTTTTATGTAAAGTTACCCTTAACAACTTAATGGTTTTCAAATAGATAG
ACATATATTTACATCTAATA

Product: O-6-alkylguanine-DNA:cysteine-protein methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 171; Mature: 171

Protein sequence:

>171_residues
MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDIHYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTL
PTATGGTPFQREVWKTLRTIPCGQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
WLLRHEGYLLL

Sequences:

>Translated_171_residues
MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDIHYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTL
PTATGGTPFQREVWKTLRTIPCGQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
WLLRHEGYLLL
>Mature_171_residues
MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDIHYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTL
PTATGGTPFQREVWKTLRTIPCGQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
WLLRHEGYLLL

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=83, Percent_Identity=50.6024096385542, Blast_Score=87, Evalue=8e-18,
Organism=Escherichia coli, GI1787596, Length=171, Percent_Identity=99.4152046783626, Blast_Score=350, Evalue=2e-98,
Organism=Escherichia coli, GI1788542, Length=89, Percent_Identity=51.685393258427, Blast_Score=104, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI115533068, Length=93, Percent_Identity=38.7096774193548, Blast_Score=77, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI115533070, Length=93, Percent_Identity=38.7096774193548, Blast_Score=77, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6320001, Length=81, Percent_Identity=48.1481481481481, Blast_Score=90, Evalue=2e-19,
Organism=Drosophila melanogaster, GI17137554, Length=105, Percent_Identity=40, Blast_Score=68, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 19165; Mature: 19165

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDIHYRKEGYERISATNPG
CCCHHHHHHCCCCCCEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPCGQVMHYGQLAEQLGRPGA
CCHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCC
ARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKEWLLRHEGYLLL
HHEECCCCCCCCEEEEEECHHHHCCCCCCCCCCCCCHHHHHHHHCCCEEEC
>Mature Secondary Structure
MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDIHYRKEGYERISATNPG
CCCHHHHHHCCCCCCEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPCGQVMHYGQLAEQLGRPGA
CCHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCC
ARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKEWLLRHEGYLLL
HHEECCCCCCCCEEEEEECHHHHCCCCCCCCCCCCCHHHHHHHHCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2825131; 9097039; 9278503; 1520330 [H]