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The map label for this gene is ycjM [H]

Identifier: 157160820

GI number: 157160820

Start: 1417666

End: 1419345

Strand: Direct

Name: ycjM [H]

Synonym: EcHS_A1424

Alternate gene names: 157160820

Gene position: 1417666-1419345 (Clockwise)

Preceding gene: 157160819

Following gene: 157160821

Centisome position: 30.53

GC content: 43.81

Gene sequence:

>1680_bases
ATGAAACAGAAAATTACGGATTACCTGGACGAAATCTACGGTGGAACATTTACCGCAACTCATTTACAGAAACTTGTAAC
GCGTCTTGAGAGTGCGAAACGATTAATTACACAGCGACGTAAAAAACACTGGGATGAAAGTGATGTCGTGTTAATTACCT
ATGCCGATCAATTTCACAGCAATGATTTAAAACCATTACCCACATTTAATCAGTTTTACCATCAATGGCTGCAAAGCATT
TTTTCACATGTTCATTTGTTGCCGTTTTATCCATGGTCATCTGATGATGGCTTTTCGGTAATTGATTATCATCAGGTCGC
CAGTGAAGCGGGGGAGTGGCAGGATATTCAGCAACTCGGTGAATGCAGTCATTTAATGTTTGATTTTGTCTGCAACCATA
TGTCGGCAAAAAGTGAATGGTTTAAAAACTATTTACAACAGCATCCAGGTTTTGAAGATTTTTTTATTGCCGTTGACCCG
CAAACCGATCTCAGCGCCGTCACTCGCCCGCGTGCGTTACCGTTATTAACGCCATTCCAGATGCGCGATCATTCAACGCG
CCATTTATGGACCACCTTTAGTGACGATCAAATTGACCTGAATTACCGTAGCCCTGAAGTGTTGCTGGCGATGGTGGATG
TTTTACTCTGTTACCTTGCGAAAGGTGCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACA
AGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGT
GATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGT
ACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCGCAAAAC
CTGACACTACCTTCCAGCAACACCACCTGGTTTAACTTCCTCGCCTCTCACGATGGCATCGGGCTAAACCCGCTACGGGG
CTTGTTGCCTGAAAGCGAAATATTAGAGCTGGTCGAGGCGTTACAGCAGGAAGGTGCATTAGTAAACTGGAAAAATAATC
CCGACGGTACACGCAGTCCGTATGAAATAAATGTGACCTATATGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAA
CGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTCTCCCCGGTGTGCCAGCGATATATATTCAAAGTATTCT
TGGCTCGCGTAATGATTACGCAGGTGTCGAAAAACTCGGATATAACCGTGCGATTAACCGTAAAAAATATCACAGTAAAG
AGATAACCCGAGAACTGAACGATGAAGCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCGT
CGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTTATCCGTAATGCGTATTCAAAGAAG
TAACGCTGATGGTAATTGTCTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATG
GTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAA

Upstream 100 bases:

>100_bases
AATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGAC
CGCTACCACGGAAAGGCAAC

Downstream 100 bases:

>100_bases
AAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAG
AAGAAAATAAAACGAATGTA

Product: alpha amylase family protein

Products: NA

Alternate protein names: Sucrose glucosyltransferase [H]

Number of amino acids: Translated: 559; Mature: 559

Protein sequence:

>559_residues
MKQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHWDESDVVLITYADQFHSNDLKPLPTFNQFYHQWLQSI
FSHVHLLPFYPWSSDDGFSVIDYHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQHPGFEDFFIAVDP
QTDLSAVTRPRALPLLTPFQMRDHSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCYLAKGAEYVRLDAVGFMWKEPGT
SCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPHKDNIAYFGAGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQN
LTLPSSNTTWFNFLASHDGIGLNPLRGLLPESEILELVEALQQEGALVNWKNNPDGTRSPYEINVTYMDALSRRESSDEE
RCARFILAHAILLSLPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKYHSKEITRELNDEATLRHAVYHELSRLITLR
RSHNEFHPDNNFTIDTINLSVMRIQRSNADGNCLTGLFNVSKNIQHVNITNLHGRDLISEVDILGNEITLRPWQVMWIK

Sequences:

>Translated_559_residues
MKQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHWDESDVVLITYADQFHSNDLKPLPTFNQFYHQWLQSI
FSHVHLLPFYPWSSDDGFSVIDYHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQHPGFEDFFIAVDP
QTDLSAVTRPRALPLLTPFQMRDHSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCYLAKGAEYVRLDAVGFMWKEPGT
SCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPHKDNIAYFGAGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQN
LTLPSSNTTWFNFLASHDGIGLNPLRGLLPESEILELVEALQQEGALVNWKNNPDGTRSPYEINVTYMDALSRRESSDEE
RCARFILAHAILLSLPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKYHSKEITRELNDEATLRHAVYHELSRLITLR
RSHNEFHPDNNFTIDTINLSVMRIQRSNADGNCLTGLFNVSKNIQHVNITNLHGRDLISEVDILGNEITLRPWQVMWIK
>Mature_559_residues
MKQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHWDESDVVLITYADQFHSNDLKPLPTFNQFYHQWLQSI
FSHVHLLPFYPWSSDDGFSVIDYHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQHPGFEDFFIAVDP
QTDLSAVTRPRALPLLTPFQMRDHSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCYLAKGAEYVRLDAVGFMWKEPGT
SCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPHKDNIAYFGAGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQN
LTLPSSNTTWFNFLASHDGIGLNPLRGLLPESEILELVEALQQEGALVNWKNNPDGTRSPYEINVTYMDALSRRESSDEE
RCARFILAHAILLSLPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKYHSKEITRELNDEATLRHAVYHELSRLITLR
RSHNEFHPDNNFTIDTINLSVMRIQRSNADGNCLTGLFNVSKNIQHVNITNLHGRDLISEVDILGNEITLRPWQVMWIK

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sucrose phosphorylase family [H]

Homologues:

Organism=Escherichia coli, GI87081873, Length=559, Percent_Identity=99.4633273703041, Blast_Score=1160, Evalue=0.0,
Organism=Drosophila melanogaster, GI24583749, Length=171, Percent_Identity=30.9941520467836, Blast_Score=85, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24583747, Length=171, Percent_Identity=30.9941520467836, Blast_Score=85, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24583745, Length=214, Percent_Identity=28.0373831775701, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI45549022, Length=177, Percent_Identity=26.5536723163842, Blast_Score=70, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006047
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR016377 [H]

Pfam domain/function: PF00128 Alpha-amylase [H]

EC number: =2.4.1.7 [H]

Molecular weight: Translated: 64210; Mature: 64210

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHWDESDVVLITYADQFHS
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
NDLKPLPTFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVIDYHQVASEAGEWQDIQQLG
CCCCCCCCHHHHHHHHHHHHHHHHEEEECCCCCCCCCCEEEHHHHHHHCCCCHHHHHHHH
ECSHLMFDFVCNHMSAKSEWFKNYLQQHPGFEDFFIAVDPQTDLSAVTRPRALPLLTPFQ
HHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHCCCCCCCCCCCHH
MRDHSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCYLAKGAEYVRLDAVGFMWKEPGT
HCCCCCCEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCC
SCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPHKDNIAYFGAGDDEAHMVYQFSLPP
CEEEEHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEEECCCCCCEEEEEECCCH
LVLHAVQKQNVEALCAWAQNLTLPSSNTTWFNFLASHDGIGLNPLRGLLPESEILELVEA
HHHHHHHHCCHHHHHHHHHHCCCCCCCCHHEEEECCCCCCCCCHHHHCCCHHHHHHHHHH
LQQEGALVNWKNNPDGTRSPYEINVTYMDALSRRESSDEERCARFILAHAILLSLPGVPA
HHHCCCEEEECCCCCCCCCCEEEEEEHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHH
IYIQSILGSRNDYAGVEKLGYNRAINRKKYHSKEITRELNDEATLRHAVYHELSRLITLR
HHHHHHHCCCCCCCHHHHHCCHHHCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
RSHNEFHPDNNFTIDTINLSVMRIQRSNADGNCLTGLFNVSKNIQHVNITNLHGRDLISE
HCCCCCCCCCCEEEEEEEEEEEEEEECCCCCCHHHHHHHHCCCCEEEEEECCCCHHHHHH
VDILGNEITLRPWQVMWIK
HHHCCCEEEECCEEEEEEC
>Mature Secondary Structure
MKQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHWDESDVVLITYADQFHS
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
NDLKPLPTFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVIDYHQVASEAGEWQDIQQLG
CCCCCCCCHHHHHHHHHHHHHHHHEEEECCCCCCCCCCEEEHHHHHHHCCCCHHHHHHHH
ECSHLMFDFVCNHMSAKSEWFKNYLQQHPGFEDFFIAVDPQTDLSAVTRPRALPLLTPFQ
HHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHCCCCCCCCCCCHH
MRDHSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCYLAKGAEYVRLDAVGFMWKEPGT
HCCCCCCEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCC
SCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPHKDNIAYFGAGDDEAHMVYQFSLPP
CEEEEHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEEECCCCCCEEEEEECCCH
LVLHAVQKQNVEALCAWAQNLTLPSSNTTWFNFLASHDGIGLNPLRGLLPESEILELVEA
HHHHHHHHCCHHHHHHHHHHCCCCCCCCHHEEEECCCCCCCCCHHHHCCCHHHHHHHHHH
LQQEGALVNWKNNPDGTRSPYEINVTYMDALSRRESSDEERCARFILAHAILLSLPGVPA
HHHCCCEEEECCCCCCCCCCEEEEEEHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHH
IYIQSILGSRNDYAGVEKLGYNRAINRKKYHSKEITRELNDEATLRHAVYHELSRLITLR
HHHHHHHCCCCCCCHHHHHCCHHHCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
RSHNEFHPDNNFTIDTINLSVMRIQRSNADGNCLTGLFNVSKNIQHVNITNLHGRDLISE
HCCCCCCCCCCEEEEEEEEEEEEEEECCCCCCHHHHHHHHCCCCEEEEEECCCCHHHHHH
VDILGNEITLRPWQVMWIK
HHHCCCEEEECCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]