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The map label for this gene is sapB

Identifier: 157160803

GI number: 157160803

Start: 1401959

End: 1402924

Strand: Reverse

Name: sapB

Synonym: EcHS_A1407

Alternate gene names: 157160803

Gene position: 1402924-1401959 (Counterclockwise)

Preceding gene: 157160804

Following gene: 157160802

Centisome position: 30.21

GC content: 51.45

Gene sequence:

>966_bases
ATGATTATCTTCACCTTACGCCGCATTTTGTTATTGATTGTCACCTTGTTCCTGCTGACCTTCGTTGGCTTCAGCTTGAG
CTATTTCACCCCTCATGCGCCGTTACAAGGCGCGTCACTGTGGAATGCCTGGGTGTTCTGGTTTAACGGCCTGATCCACT
GGGATTTTGGTGTTTCCAGCATTAACGGTCAACCGATTGCCGAACAGTTAAAAGAGGTCTTCCCGGCGACGATGGAACTG
TGCATCCTCGCCTTCGGTTTTGCGCTGATTGTTGGCATCCCCGTGGGGATGATTGCCGGGATTACGCGCCATAAGTGGCA
GGATAACTTGATCAACGCCATCGCCTTACTGGGCTTTTCAATCCCGGTGTTCTGGCTGGCGCTTCTGTTGACGCTGTTTT
GTTCACTCACGCTAGGTTGGTTGCCCGTTTCAGGGCGTTTCGATCTGCTCTACGAAGTGAAACCGATTACCGGTTTTGCG
TTGATTGATGCCTGGCTTTCGGACTCACCATGGCGGGATGAAATGATCATGAGCGCAATCCGCCATATGATATTGCCCGT
GATCACCCTGTCGGTTGCGCCAACAACTGAAGTGATCCGCCTGATGCGTATCAGCACTATCGAAGTGTATGACCAGAACT
ATGTAAAAGCGGCGGCGACCCGCGGTTTGTCGCGCTTTACTATTTTGCGTCGCCACGTTCTGCATAACGCGTTACCGCCG
GTTATTCCTCGCCTGGGCTTACAGTTTTCCACCATGCTGACGCTGGCGATGATCACCGAAATGGTTTTTAGCTGGCCGGG
TTTAGGGCGCTGGTTAATTAACGCCATCCGCCAGCAGGATTACGCAGCCATTTCCGCCGGAGTGATGGTGTGTGGCTCAC
TGGTCATTATTGTTAACGTGATTTCTGATATTTTGGGTGCCATGGCTAACCCTCTGAAACATAAGGAATGGTATGCCTTA
CGATAG

Upstream 100 bases:

>100_bases
AGGCCTATCGGTACGATATCAAAGGTCTGGTACTTAGCCCGTTTGGTAACGCCTCCTTTGCTGGGGTGTATCGCGAGAAA
CAGGATGAGGTGAAAAAACC

Downstream 100 bases:

>100_bases
CGTATACAGCGAAAAGCGCCCGCCGGGCACGCTGCGTACCGCCTGGCGCAAATTTTATAGTGACGCCTCTGCGATGGTCG
GCCTGTACGGCTGCGCGGGG

Product: peptide ABC transporter, permease protein SapB

Products: ADP; phosphate; peptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAWVFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMEL
CILAFGFALIVGIPVGMIAGITRHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAAATRGLSRFTILRRHVLHNALPP
VIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAIRQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYAL
R

Sequences:

>Translated_321_residues
MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAWVFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMEL
CILAFGFALIVGIPVGMIAGITRHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAAATRGLSRFTILRRHVLHNALPP
VIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAIRQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYAL
R
>Mature_321_residues
MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAWVFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMEL
CILAFGFALIVGIPVGMIAGITRHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAAATRGLSRFTILRRHVLHNALPP
VIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAIRQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYAL
R

Specific function: Involved in a peptide intake transport system that plays a role in the resistance to antimicrobial peptides

COG id: COG4168

COG function: function code V; ABC-type antimicrobial peptide transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain

Homologues:

Organism=Escherichia coli, GI1787550, Length=321, Percent_Identity=100, Blast_Score=645, Evalue=0.0,
Organism=Escherichia coli, GI1789965, Length=344, Percent_Identity=35.4651162790698, Blast_Score=214, Evalue=6e-57,
Organism=Escherichia coli, GI1787053, Length=337, Percent_Identity=29.080118694362, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1787761, Length=239, Percent_Identity=36.8200836820084, Blast_Score=144, Evalue=8e-36,
Organism=Escherichia coli, GI1789888, Length=345, Percent_Identity=29.8550724637681, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1787497, Length=331, Percent_Identity=28.0966767371601, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1788504, Length=261, Percent_Identity=26.0536398467433, Blast_Score=81, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SAPB_ECO57 (P0AGH4)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   C85756
- PIR:   F90862
- RefSeq:   NP_287895.1
- RefSeq:   NP_309897.1
- ProteinModelPortal:   P0AGH4
- EnsemblBacteria:   EBESCT00000026133
- EnsemblBacteria:   EBESCT00000059069
- GeneID:   912731
- GeneID:   961349
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z2496
- KEGG:   ecs:ECs1870
- GeneTree:   EBGT00050000009815
- HOGENOM:   HBG724229
- OMA:   ATIELCI
- ProtClustDB:   PRK15110
- BioCyc:   ECOL83334:ECS1870-MONOMER
- InterPro:   IPR000515

Pfam domain/function: PF00528 BPD_transp_1

EC number: NA

Molecular weight: Translated: 36039; Mature: 36039

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x13e15e14)-; HASH(0x145f6244)-; HASH(0x1386939c)-; HASH(0x12f12580)-; HASH(0x12942e3c)-; HASH(0x1398907c)-;

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAWVFWFNGLIHWDFGVSS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHEECCCCCC
INGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGITRHKWQDNLINAIALLGFS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFALIDAWLSDSPWRDEMIMSAI
HHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHH
RHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAAATRGLSRFTILRRHVLHNALPP
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCH
VIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAIRQQDYAAISAGVMVCGSLVIIVNV
HHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISDILGAMANPLKHKEWYALR
HHHHHHHHCCCCCCCCCCCCH
>Mature Secondary Structure
MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAWVFWFNGLIHWDFGVSS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHEECCCCCC
INGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGITRHKWQDNLINAIALLGFS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFALIDAWLSDSPWRDEMIMSAI
HHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHH
RHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAAATRGLSRFTILRRHVLHNALPP
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCH
VIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAIRQQDYAAISAGVMVCGSLVIIVNV
HHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISDILGAMANPLKHKEWYALR
HHHHHHHHCCCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; peptides [Periplasm]; H2O [C]

Specific reaction: ATP + peptides [Periplasm] + H2O = ADP + phosphate + peptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796