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The map label for this gene is sapD

Identifier: 157160801

GI number: 157160801

Start: 1400090

End: 1401082

Strand: Reverse

Name: sapD

Synonym: EcHS_A1405

Alternate gene names: 157160801

Gene position: 1401082-1400090 (Counterclockwise)

Preceding gene: 157160802

Following gene: 157160800

Centisome position: 30.17

GC content: 51.16

Gene sequence:

>993_bases
ATGCCATTACTCGATATTCGTAACCTGACCATTGAATTTAAAACCGGTGATGAGTGGGTTAAAGCCGTCGACCGGGTAAG
TATGACCTTAACCGAAGGTGAAATCCGCGGTCTTGTTGGTGAATCCGGTTCCGGCAAGAGTTTGATTGCGAAAGCGATTT
GTGGGGTGAATAAAGATAACTGGCGTGTTACTGCTGACCGTATGCGTTTTGATGATATCGATTTGCTGCGTCTCTCCGCA
CGCGAACGGCGCAAACTGGTTGGCCATAACGTGTCGATGATTTTCCAGGAACCGCAGTCGTGTCTTGACCCTTCAGAACG
TGTGGGTCGCCAGTTGATGCAAAACATCCCAGCCTGGACCTATAAAGGCCGTTGGTGGCAGCGTTTTGGCTGGCGCAAAC
GCCGTGCCATTGAACTGCTGCACCGCGTGGGGATTAAAGATCACAAAGATGCGATGCGCAGTTTTCCCTATGAGTTGACC
GAAGGTGAATGTCAGAAAGTGATGATAGCCATTGCACTGGCGAATCAACCGCGTCTGCTGATTGCTGACGAACCGACCAA
CTCAATGGAGCCAACAACCCAGGCGCAAATCTTTCGCCTGCTGACGCGTCTCAACCAAAACAGCAATACCACTATTTTGC
TTATCAGCCATGACTTACAAATGCTTAGCCAATGGGCGGATAAAATTAACGTGCTTTACTGCGGTCAAACAGTGGAAACC
GCGCCGAGTAAGGAGTTGGTGACGATGCCACATCATCCTTATACCCAGGCGCTGATCCGCGCGATACCAGACTTCGGCAG
CGCGATGCCGCATAAAAGTCGCCTCAATACGCTACCCGGCGCTATCCCACTGCTGGAACAGTTACCGATTGGTTGTCGTC
TGGGGCCACGTTGCCCGTATGCACAACGAGAATGCATTGTGACGCCACGTTTGACGGGGGCGAAAAATCATCTCTATGCC
TGTCATTTCCCGCTGAACATGGAGAAAGAGTGA

Upstream 100 bases:

>100_bases
GGACTGTCATGCTGCCAGGTGCGGCAATTATGATTAGCGTGTTGTTGGTTAACCTCCTTGGCGATGGCGTTCGTCGTGCG
ATTATTGCGGGGGTGGAATA

Downstream 100 bases:

>100_bases
GATGATCGAAACGCTGCTTGAAGTGCGTAATTTAAGTAAAACGTTCCGCTACCGGACCGGCTGGTTTCGTCGTCAGACCG
TAGAAGCGGTAAAACCCTTG

Product: peptide ABC transporter, ATP-binding protein SapD

Products: ADP; phosphate; peptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGSGKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSA
RERRKLVGHNVSMIFQEPQSCLDPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHDLQMLSQWADKINVLYCGQTVET
APSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLNTLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYA
CHFPLNMEKE

Sequences:

>Translated_330_residues
MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGSGKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSA
RERRKLVGHNVSMIFQEPQSCLDPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHDLQMLSQWADKINVLYCGQTVET
APSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLNTLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYA
CHFPLNMEKE
>Mature_329_residues
PLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGSGKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSAR
ERRKLVGHNVSMIFQEPQSCLDPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELTE
GECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHDLQMLSQWADKINVLYCGQTVETA
PSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLNTLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYAC
HFPLNMEKE

Specific function: Involved in a peptide intake transport system that plays a role in the resistance to antimicrobial peptides

COG id: COG4170

COG function: function code V; ABC-type antimicrobial peptide transport system, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain

Homologues:

Organism=Escherichia coli, GI1787548, Length=330, Percent_Identity=100, Blast_Score=687, Evalue=0.0,
Organism=Escherichia coli, GI1789963, Length=328, Percent_Identity=43.9024390243902, Blast_Score=270, Evalue=8e-74,
Organism=Escherichia coli, GI1787759, Length=328, Percent_Identity=37.5, Blast_Score=211, Evalue=8e-56,
Organism=Escherichia coli, GI48994883, Length=324, Percent_Identity=37.037037037037, Blast_Score=207, Evalue=7e-55,
Organism=Escherichia coli, GI1787500, Length=329, Percent_Identity=33.7386018237082, Blast_Score=172, Evalue=2e-44,
Organism=Escherichia coli, GI145693107, Length=271, Percent_Identity=33.9483394833948, Blast_Score=165, Evalue=5e-42,
Organism=Escherichia coli, GI1788506, Length=270, Percent_Identity=35.9259259259259, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI1787758, Length=316, Percent_Identity=31.0126582278481, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1789962, Length=307, Percent_Identity=29.9674267100977, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1787547, Length=274, Percent_Identity=28.1021897810219, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1786398, Length=290, Percent_Identity=29.3103448275862, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI48994997, Length=263, Percent_Identity=29.277566539924, Blast_Score=110, Evalue=9e-26,
Organism=Escherichia coli, GI1789890, Length=241, Percent_Identity=29.045643153527, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1789891, Length=259, Percent_Identity=29.7297297297297, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI87081834, Length=229, Percent_Identity=30.5676855895196, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787105, Length=250, Percent_Identity=28.8, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1789873, Length=221, Percent_Identity=30.7692307692308, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1789032, Length=245, Percent_Identity=26.530612244898, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI87081791, Length=270, Percent_Identity=26.2962962962963, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1788225, Length=262, Percent_Identity=26.3358778625954, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1790544, Length=240, Percent_Identity=28.75, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1786703, Length=241, Percent_Identity=27.8008298755187, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1788450, Length=239, Percent_Identity=25.5230125523013, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787712, Length=263, Percent_Identity=25.0950570342205, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1787792, Length=245, Percent_Identity=30.2040816326531, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1789672, Length=266, Percent_Identity=26.3157894736842, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1786872, Length=214, Percent_Identity=27.1028037383178, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787029, Length=243, Percent_Identity=24.2798353909465, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1790162, Length=260, Percent_Identity=22.6923076923077, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI48995001, Length=241, Percent_Identity=30.2904564315353, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1787370, Length=255, Percent_Identity=24.3137254901961, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1789864, Length=257, Percent_Identity=24.124513618677, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787089, Length=219, Percent_Identity=26.4840182648402, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1788644, Length=129, Percent_Identity=27.1317829457364, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1788210, Length=266, Percent_Identity=26.6917293233083, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788472, Length=228, Percent_Identity=27.6315789473684, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI87082267, Length=248, Percent_Identity=22.9838709677419, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790467, Length=259, Percent_Identity=24.3243243243243, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SAPD_ECO57 (P0AAH6)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   D90862
- PIR:   E85756
- RefSeq:   NP_287897.1
- RefSeq:   NP_309895.1
- ProteinModelPortal:   P0AAH6
- SMR:   P0AAH6
- EnsemblBacteria:   EBESCT00000026817
- EnsemblBacteria:   EBESCT00000057786
- GeneID:   912736
- GeneID:   961351
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z2499
- KEGG:   ecs:ECs1868
- GeneTree:   EBGT00070000031696
- HOGENOM:   HBG758042
- OMA:   MSKWADR
- ProtClustDB:   PRK15093
- BioCyc:   ECOL83334:ECS1868-MONOMER
- InterPro:   IPR003439
- InterPro:   IPR003593
- InterPro:   IPR010066
- InterPro:   IPR013563
- SMART:   SM00382
- TIGRFAMs:   TIGR01727

Pfam domain/function: PF00005 ABC_tran; PF08352 oligo_HPY

EC number: NA

Molecular weight: Translated: 37661; Mature: 37530

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: PS00211 ABC_TRANSPORTER_1; PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGSGKSLIAKAICGVNKDN
CCCEEEECEEEEEECCHHHHHHHHHHHEEEECCHHHHCCCCCCCCHHHHHHHHHCCCCCC
WRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCLDPSERVGRQLMQNIPAWT
CEEEHHCCCCCCHHHHHHHHHHHHHHHCCCHHHEEECCHHHCCHHHHHHHHHHHHCCCCE
YKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELTEGECQKVMIAIALANQPRLL
ECCCHHHHHCHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCHHHEEEEEEEECCCCEEE
IADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHDLQMLSQWADKINVLYCGQTVET
EEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHCEEEEECCCCCC
APSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLNTLPGAIPLLEQLPIGCRLGPRCPY
CCCCCEEECCCCHHHHHHHHHCCHHCCCCCCHHHHHCCCCCHHHHHHCCCCCCCCCCCCC
AQRECIVTPRLTGAKNHLYACHFPLNMEKE
CCCCEEECCCCCCCCCCEEEEECCCCCCCC
>Mature Secondary Structure 
PLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGSGKSLIAKAICGVNKDN
CCEEEECEEEEEECCHHHHHHHHHHHEEEECCHHHHCCCCCCCCHHHHHHHHHCCCCCC
WRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCLDPSERVGRQLMQNIPAWT
CEEEHHCCCCCCHHHHHHHHHHHHHHHCCCHHHEEECCHHHCCHHHHHHHHHHHHCCCCE
YKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELTEGECQKVMIAIALANQPRLL
ECCCHHHHHCHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCHHHEEEEEEEECCCCEEE
IADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHDLQMLSQWADKINVLYCGQTVET
EEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHCEEEEECCCCCC
APSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLNTLPGAIPLLEQLPIGCRLGPRCPY
CCCCCEEECCCCHHHHHHHHHCCHHCCCCCCHHHHHCCCCCHHHHHHCCCCCCCCCCCCC
AQRECIVTPRLTGAKNHLYACHFPLNMEKE
CCCCEEECCCCCCCCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; peptides [Periplasm]; H2O [C]

Specific reaction: ATP + peptides [Periplasm] + H2O = ADP + phosphate + peptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796