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The map label for this gene is yciA [H]

Identifier: 157160758

GI number: 157160758

Start: 1358905

End: 1359303

Strand: Reverse

Name: yciA [H]

Synonym: EcHS_A1362

Alternate gene names: 157160758

Gene position: 1359303-1358905 (Counterclockwise)

Preceding gene: 157160759

Following gene: 157160756

Centisome position: 29.27

GC content: 48.62

Gene sequence:

>399_bases
ATGTCTACAACACATAACGTCCCTCAGGGCGATCTTGTTTTACGTACTTTAGCCATGCCCGCCGATACCAATGCCAATGG
TGACATCTTTGGTGGTTGGTTAATGTCACAAATGGATATTGGCGGCGCTATTCTGGCAAAAGAAATTGCCCACGGTCGCG
TAGTGACTGTGCGGGTTGAAGGAATGACTTTCTTACGGCCGGTTGCGGTCGGCGATGTGGTGTGCTGCTATGCACGCTGT
GTCCAGAAAGGGACGACATCGGTCAGCATTAATATTGAAGTGTGGGTGAAAAAAGTAGCGTCTGAACCAATCGGGCAACG
GTATAAAGCGACAGAAGCATTATTTAAGTATGTCGCGGTTGATCCTGAAGGAAAACCTCGCGCCTTACCTGTTGAGTAA

Upstream 100 bases:

>100_bases
ACTGCCAGTCCTTCGGGGCTGGCGCTGTATCTCCTCTGTTCTCCTCAGGAAAATCATAGTAGCATCGCGCCTGTGATTTT
CCTTTTAAGTCGGTTTTACC

Downstream 100 bases:

>100_bases
TAGTCAAAAGCCTCCGGTCGGAGGCTTTTGACTTTATGCTTACTGAATTTCGGTGGTGCCGTTAATTTTAAACAGGATAT
TCACCACAATCCCACTGCCT

Product: acyl-CoA thioester hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 132; Mature: 131

Protein sequence:

>132_residues
MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAILAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARC
VQKGTTSVSINIEVWVKKVASEPIGQRYKATEALFKYVAVDPEGKPRALPVE

Sequences:

>Translated_132_residues
MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAILAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARC
VQKGTTSVSINIEVWVKKVASEPIGQRYKATEALFKYVAVDPEGKPRALPVE
>Mature_131_residues
STTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAILAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCV
QKGTTSVSINIEVWVKKVASEPIGQRYKATEALFKYVAVDPEGKPRALPVE

Specific function: Catalyzes the hydrolysis of the thioester bond in palmitoyl-CoA and malonyl-CoA [H]

COG id: COG1607

COG function: function code I; Acyl-CoA hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acyl coenzyme A hydrolase family [H]

Homologues:

Organism=Escherichia coli, GI1787506, Length=132, Percent_Identity=100, Blast_Score=273, Evalue=2e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006683 [H]

Pfam domain/function: PF03061 4HBT [H]

EC number: 3.1.2.- [C]

Molecular weight: Translated: 14231; Mature: 14100

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAILAKEIAHGRVVTVRVE
CCCCCCCCCCCEEEEEECCCCCCCCCCCEEHHHHHHHHCCCHHHHHHHHCCCCEEEEEEC
GMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASEPIGQRYKATEALFKYVAV
CEEEEHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHCCCCCCHHHHHHHHHHHEEE
DPEGKPRALPVE
CCCCCCCCCCCC
>Mature Secondary Structure 
STTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAILAKEIAHGRVVTVRVE
CCCCCCCCCCEEEEEECCCCCCCCCCCEEHHHHHHHHCCCHHHHHHHHCCCCEEEEEEC
GMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASEPIGQRYKATEALFKYVAV
CEEEEHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHCCCCCCHHHHHHHHHHHEEE
DPEGKPRALPVE
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds; Thiolester hydrolases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]