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The map label for this gene is cls [H]

Identifier: 157160754

GI number: 157160754

Start: 1354242

End: 1355702

Strand: Reverse

Name: cls [H]

Synonym: EcHS_A1358

Alternate gene names: 157160754

Gene position: 1355702-1354242 (Counterclockwise)

Preceding gene: 157160755

Following gene: 157160753

Centisome position: 29.2

GC content: 51.61

Gene sequence:

>1461_bases
ATGACAACCGTTTATACGTTGGTGAGTTGGTTGGCCATTCTGGGATACTGGTTGCTCATTGCAGGCGTAACTTTACGCAT
TCTAATGAAACGACGCGCAGTTCCCTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTG
CCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCCGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAA
TGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTGCGCCATTATTCAAGCTTTG
CGAGCGTCGTCAGGGGATCGCTGGGGTCAAAGGGAATCAGCTACAACTGATGACCGAGTCAGATGATGTGATGCAGGCGT
TAATCCGCGACATCCAGCTCGCGCGCCATAATATTGAGATGGTGTTTTATATCTGGCAGCCCGGCGGCATGGCGGACCAG
GTGGCTGAATCATTAATGGCCGCTGCGCGACGCGGCATTCATTGCCGATTGATGCTCGACTCCGCCGGGAGTGTGGCTTT
TTTCCGCAGCCCGTGGCCCGAGCTAATGCGTAATGCCGGTATTGAAGTGGTCGAAGCCTTAAAGGTCAATCTGATGCGTG
TGTTTTTACGCCGTATGGACCTGCGCCAACATCGCAAGATGATCATGATCGATAATTACATCGCGTACACCGGCAGCATG
AATATGGTCGACCCTCGCTACTTCAAACAAGATGCGGGCGTAGGGCAATGGATTGATCTGATGGCGCGTATGGAAGGCCC
CATCGCCACCGCGATGGGGATTATTTATTCCTGCGACTGGGAGATTGAAACCGGAAAACGTATTCTGCCGCCACCACCAG
ATGTCAATATTATGCCGTTTGAACAGGCCAGCGGTCACACCATTCACACAATTGCTTCTGGCCCAGGCTTTCCGGAAGAT
CTCATTCACCAGGCATTATTGACTGCGGCTTATTCGGCGCGTGAATATTTGATCATGACAACGCCCTACTTTGTGCCAAG
CGATGATTTACTTCATGCGATTTGCACGGCGGCACAGCGCGGGGTGGATGTCAGTATTATCCTTCCGCGAAAAAATGACT
CGATGCTGGTCGGCTGGGCCAGTCGCGCATTCTTTACGGAACTGCTGGCTGCTGGGGTTAAAATTTATCAGTTTGAAGGC
GGGTTACTGCATACCAAGAGCGTGCTGGTCGATGGTGAACTAAGTCTGGTTGGCACCGTTAACCTTGATATGCGGAGTCT
GTGGCTAAATTTCGAGATTACTCTGGCAATCGACGATAAAGGTTTTGGTGCAGACCTTGCCGCCGTTCAGGACGATTATA
TTTCGCGTTCACGTCTGCTCGATGCCCGTTTATGGCTAAAACGTCCATTATGGCAACGTGTCGCCGAGCGACTGTTTTAC
TTCTTCAGCCCGTTGCTGTAA

Upstream 100 bases:

>100_bases
GCCCCCGACGCTGACCAGTGCTTTTCCCGCGTTTTTATTTGCCAGATGAATCCATATGCGTAAACTCATAACAATGCGCT
TTCAAAAGGATTTCTAATCT

Downstream 100 bases:

>100_bases
AACGTCGCCAACAGAGGTTAAACAGGTAGTGACTATGGATATGGATCTAAACAATCGCCTGACTGAAGATGAAACGCTTG
AGCAGGCCTACGATATTTTT

Product: cardiolipin synthetase

Products: NA

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLIIYILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAK
WLNDLKACKHIFAEENSSVAAPLFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRRMDLRQHRKMIMIDNYIAYTGSM
NMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIYSCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPED
LIHQALLTAAYSAREYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAGVKIYQFEG
GLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQDDYISRSRLLDARLWLKRPLWQRVAERLFY
FFSPLL

Sequences:

>Translated_486_residues
MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLIIYILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAK
WLNDLKACKHIFAEENSSVAAPLFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRRMDLRQHRKMIMIDNYIAYTGSM
NMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIYSCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPED
LIHQALLTAAYSAREYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAGVKIYQFEG
GLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQDDYISRSRLLDARLWLKRPLWQRVAERLFY
FFSPLL
>Mature_485_residues
TTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLIIYILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKW
LNDLKACKHIFAEENSSVAAPLFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQV
AESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRRMDLRQHRKMIMIDNYIAYTGSMN
MVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIYSCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDL
IHQALLTAAYSAREYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAGVKIYQFEGG
LLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQDDYISRSRLLDARLWLKRPLWQRVAERLFYF
FSPLL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=486, Percent_Identity=100, Blast_Score=997, Evalue=0.0,
Organism=Escherichia coli, GI1787007, Length=400, Percent_Identity=25.25, Blast_Score=103, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 54823; Mature: 54692

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLIIYILPLVGIIAYLAVGE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAPLFKLCERRQGIAGVKGNQ
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCE
LQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQVAESLMAAARRGIHCRLMLD
EEEEECCHHHHHHHHHHHHHHHCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEEE
SAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRRMDLRQHRKMIMIDNYIAYTGSM
CCCCEEEECCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECEEEEECCC
NMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIYSCDWEIETGKRILPPPPDVNIMPF
CCCCCHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHEEECEEECCCCCCCCCCCCCEEEEC
EQASGHTIHTIASGPGFPEDLIHQALLTAAYSAREYLIMTTPYFVPSDDLLHAICTAAQR
CCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHC
GVDVSIILPRKNDSMLVGWASRAFFTELLAAGVKIYQFEGGLLHTKSVLVDGELSLVGTV
CCEEEEEEECCCCCEEEEEHHHHHHHHHHHCCCEEEEECCCEEEECEEEECCCEEEEEEE
NLDMRSLWLNFEITLAIDDKGFGADLAAVQDDYISRSRLLDARLWLKRPLWQRVAERLFY
CCCCEEEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
FFSPLL
HHHCCC
>Mature Secondary Structure 
TTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLIIYILPLVGIIAYLAVGE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAPLFKLCERRQGIAGVKGNQ
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCE
LQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQVAESLMAAARRGIHCRLMLD
EEEEECCHHHHHHHHHHHHHHHCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEEE
SAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRRMDLRQHRKMIMIDNYIAYTGSM
CCCCEEEECCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECEEEEECCC
NMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIYSCDWEIETGKRILPPPPDVNIMPF
CCCCCHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHEEECEEECCCCCCCCCCCCCEEEEC
EQASGHTIHTIASGPGFPEDLIHQALLTAAYSAREYLIMTTPYFVPSDDLLHAICTAAQR
CCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHC
GVDVSIILPRKNDSMLVGWASRAFFTELLAAGVKIYQFEGGLLHTKSVLVDGELSLVGTV
CCEEEEEEECCCCCEEEEEHHHHHHHHHHHCCCEEEEECCCEEEECEEEECCCEEEEEEE
NLDMRSLWLNFEITLAIDDKGFGADLAAVQDDYISRSRLLDARLWLKRPLWQRVAERLFY
CCCCEEEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
FFSPLL
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA