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The map label for this gene is hns

Identifier: 157160743

GI number: 157160743

Start: 1340767

End: 1341180

Strand: Reverse

Name: hns

Synonym: EcHS_A1345

Alternate gene names: 157160743

Gene position: 1341180-1340767 (Counterclockwise)

Preceding gene: 157160744

Following gene: 157160739

Centisome position: 28.88

GC content: 46.62

Gene sequence:

>414_bases
ATGAGCGAAGCACTTAAAATTCTGAACAACATCCGTACTCTTCGTGCGCAGGCAAGAGAATGTACACTTGAAACGCTGGA
AGAAATGCTGGAAAAATTAGAAGTTGTTGTTAACGAACGTCGCGAAGAAGAAAGCGCGGCTGCTGCTGAAGTTGAAGAGC
GCACTCGTAAACTGCAGCAATATCGCGAAATGCTGATCGCTGACGGTATTGACCCGAACGAACTGCTGAATAGCCTTGCT
GCCGTTAAATCTGGCACCAAAGCTAAACGTGCTCAGCGTCCGGCAAAATATAGCTACGTTGACGAAAACGGCGAAACTAA
AACCTGGACTGGCCAAGGCCGTACTCCAGCTGTAATCAAAAAAGCAATGGATGAGCAAGGTAAATCCCTCGACGATTTCC
TGATCAAGCAATAA

Upstream 100 bases:

>100_bases
CAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAAT
ATAAGTTTGAGATTACTACA

Downstream 100 bases:

>100_bases
TCTTTTGTAGATTGCACTTGCTTAAAATCCCGCCAGCGGCGGGATTTTTTATTGTCCGGTTTAAGACAATTTAATAAGAA
TAAGCTATAAAAAAACGGCG

Product: global DNA-binding transcriptional dual regulator H-NS

Products: NA

Alternate protein names: Histone-like protein HLP-II; Protein B1; Protein H1

Number of amino acids: Translated: 137; Mature: 136

Protein sequence:

>137_residues
MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEESAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLA
AVKSGTKAKRAQRPAKYSYVDENGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ

Sequences:

>Translated_137_residues
MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEESAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLA
AVKSGTKAKRAQRPAKYSYVDENGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ
>Mature_136_residues
SEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEESAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAA
VKSGTKAKRAQRPAKYSYVDENGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ

Specific function: H-NS binds tightly to ds-DNA, increases its thermal stability and inhibits transcription. It also binds to ss-DNA and RNA but with a much lower affinity. H-NS has possible histone-like function. May be a global transcriptional regulator through its abilit

COG id: COG2916

COG function: function code R; DNA-binding protein H-NS

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the histone-like protein H-NS family

Homologues:

Organism=Escherichia coli, GI1787489, Length=137, Percent_Identity=100, Blast_Score=276, Evalue=3e-76,
Organism=Escherichia coli, GI1789023, Length=135, Percent_Identity=57.7777777777778, Blast_Score=135, Evalue=1e-33,

Paralogues:

None

Copy number: 820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1780 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 1020 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 4632 Molecules/Cell In: Gr

Swissprot (AC and ID): HNS_ECO57 (P0ACG0)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   B85704
- PIR:   C90846
- RefSeq:   NP_287482.1
- RefSeq:   NP_309766.1
- ProteinModelPortal:   P0ACG0
- MINT:   MINT-1223888
- EnsemblBacteria:   EBESCT00000026108
- EnsemblBacteria:   EBESCT00000060060
- GeneID:   913113
- GeneID:   960489
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z2013
- KEGG:   ecs:ECs1739
- GeneTree:   EBGT00050000008684
- HOGENOM:   HBG740436
- OMA:   QYRDMLI
- ProtClustDB:   PRK10947
- BioCyc:   ECOL83334:ECS1739-MONOMER
- GO:   GO:0005622
- GO:   GO:0006350
- InterPro:   IPR001801
- SMART:   SM00528

Pfam domain/function: PF00816 Histone_HNS

EC number: NA

Molecular weight: Translated: 15540; Mature: 15409

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEESAAAAEVEERTRKLQQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDENGETKTWTGQGRTPAVIK
HHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHH
KAMDEQGKSLDDFLIKQ
HHHHHCCCCHHHHHHCC
>Mature Secondary Structure 
SEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEESAAAAEVEERTRKLQQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDENGETKTWTGQGRTPAVIK
HHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHH
KAMDEQGKSLDDFLIKQ
HHHHHCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796