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The map label for this gene is dhaR [H]

Identifier: 157160705

GI number: 157160705

Start: 1299505

End: 1301424

Strand: Direct

Name: dhaR [H]

Synonym: EcHS_A1305

Alternate gene names: 157160705

Gene position: 1299505-1301424 (Clockwise)

Preceding gene: 157160701

Following gene: 157160707

Centisome position: 27.99

GC content: 51.46

Gene sequence:

>1920_bases
ATGAGTGGCGCTTTTAACAACGATGGTCGGGGCATATCTCCCTTAATTGCAACCTCCTGGGAGCGATGCAATAAGCTGAT
GAAACGGGAGACATGGAACGTACCACATCAGGCCCAGGGCGTGACATTTGCTTCTATTTATCGGCGTAAGAAAGCGATGC
TGACGCTCGGGCAGGCTGCGCTGGAAGATGCCTGGGAATATATGGCACCGCGAGAGTGTGCGCTGTTTATCCTCGATGAA
ACCGCCTGCATTCTCAGCCGTAATGGCGATCCGCAAACCTTGCAGCAGCTAAGTGCACTGGGATTCAATGACGGCACGTA
TTGCGCCGAGGGAATTATTGGTACTTGTGCGCTATCGTTAGCGGCTATCTCTGGTCAGGCCGTGAAAACGATGGCCGATC
AACATTTCAAACAGGCACTCTGGAACTGGGCCTTTTGTGCAACGCCGTTGTTTGACAGCAAGGGCCGATTGACGGGAACA
ATAGCGCTGGCGTGTCCGGTTGAGCAAACTACCGCAGCTGATTTGCCGTTGACGTTGGCAATCGCCCGCGAGGTCGGAAA
TTTACTGCTGACGGACAGTTTGCTCGCTGAAACTAACCGTCATTTAAATCAACTTAATGCCCTGTTAGAAAGTATGGATG
ATGGCGTGATTAGCTGGGATGAGCAGGGTAATTTGCAATTTATTAATGCCCAGGCGGCGCGGGTCTTGCGCCTTGACGCG
ACGGCAAGTCAGGGACGGGCAATCACTGAACTCTTAACGTTACCCGCCGTATTGCAACAAGCAATAAAACAGGCACATCC
GCTCAAACACGTAGAAGCAACCTTTGAAAGCCAGCACCAGTTTATTGATGCGGTGATAACCCTTAAACCGATAATAGAAA
CGCAGGGAACCAGCTTTATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTAATGACCAGTCAATTAGGAAAAGTC
AGCCATACCTTCGCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAG
TAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGCACTGCTAAGTCAGGCAATTCATAATGAAAGCGAGC
GTGCTGCAGGTCCTTATATCGCCGTCAATTGTGAGTTATATGGTGATGCTGCGCTGGCGGAAGAATTTATTGGTGGCGAT
CGCACGGACAATGAAAATGGCCGTCTGAGTCGGCTGGAACTGGCACACGGCGGCACGCTGTTTCTTGAAAAGATTGAATA
TCTGGCGGTGGAGTTACAGTCTGCTTTGCTTCAGGTTATCAAGCAGGGGGTTATCACGCGACTGGATGCGCGGCGTTTAA
TACCAATTGATGTCAAAGTGATTGCCACAACGACCGCGGACCTCGCAATGCTGGTGGAACAAAATCGTTTTAGTCGCCAG
CTGTATTACGCGCTGCATGCATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAA
TAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTCTT
GTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGC
GTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTC
ATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTAC
TTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCAGGGCAGTTTAAGCGCCGGGTATGA

Upstream 100 bases:

>100_bases
GAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTT
TGTCATAAGGGATGCGGGAT

Downstream 100 bases:

>100_bases
AAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAA
GGGCTTGTCTATCCCGCACT

Product: DNA-binding transcriptional regulator DhaR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 639; Mature: 638

Protein sequence:

>639_residues
MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFASIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDE
TACILSRNGDPQTLQQLSALGFNDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVISWDEQGNLQFINAQAARVLRLDA
TASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFESQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKV
SHTFAHMPQDDPQTRRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAALAEEFIGGD
RTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDARRLIPIDVKVIATTTADLAMLVEQNRFSRQ
LYYALHAFEITIPPLRMRRGSIPALVNNKLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIR
VSDLPEHLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRKMKQHGIDAGQFKRRV

Sequences:

>Translated_639_residues
MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFASIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDE
TACILSRNGDPQTLQQLSALGFNDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVISWDEQGNLQFINAQAARVLRLDA
TASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFESQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKV
SHTFAHMPQDDPQTRRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAALAEEFIGGD
RTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDARRLIPIDVKVIATTTADLAMLVEQNRFSRQ
LYYALHAFEITIPPLRMRRGSIPALVNNKLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIR
VSDLPEHLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRKMKQHGIDAGQFKRRV
>Mature_638_residues
SGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFASIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDET
ACILSRNGDPQTLQQLSALGFNDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGTI
ALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVISWDEQGNLQFINAQAARVLRLDAT
ASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFESQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVS
HTFAHMPQDDPQTRRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAALAEEFIGGDR
TDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDARRLIPIDVKVIATTTADLAMLVEQNRFSRQL
YYALHAFEITIPPLRMRRGSIPALVNNKLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRV
SDLPEHLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRKMKQHGIDAGQFKRRV

Specific function: Positively regulates the dhaKLM operon from a sigma-70 promoter. Represses its own synthesis [H]

COG id: COG3284

COG function: function code QK; Transcriptional activator of acetoin/glycerol metabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87081858, Length=639, Percent_Identity=99.8435054773083, Blast_Score=1313, Evalue=0.0,
Organism=Escherichia coli, GI87082117, Length=306, Percent_Identity=35.2941176470588, Blast_Score=156, Evalue=5e-39,
Organism=Escherichia coli, GI1790299, Length=312, Percent_Identity=32.0512820512821, Blast_Score=149, Evalue=6e-37,
Organism=Escherichia coli, GI1788550, Length=325, Percent_Identity=31.0769230769231, Blast_Score=149, Evalue=7e-37,
Organism=Escherichia coli, GI1788905, Length=314, Percent_Identity=33.1210191082803, Blast_Score=148, Evalue=1e-36,
Organism=Escherichia coli, GI1789233, Length=450, Percent_Identity=28.8888888888889, Blast_Score=145, Evalue=8e-36,
Organism=Escherichia coli, GI1789087, Length=326, Percent_Identity=31.9018404907975, Blast_Score=140, Evalue=3e-34,
Organism=Escherichia coli, GI1790437, Length=287, Percent_Identity=33.1010452961672, Blast_Score=139, Evalue=7e-34,
Organism=Escherichia coli, GI1786524, Length=309, Percent_Identity=31.7152103559871, Blast_Score=133, Evalue=4e-32,
Organism=Escherichia coli, GI1787583, Length=337, Percent_Identity=26.4094955489614, Blast_Score=115, Evalue=1e-26,
Organism=Escherichia coli, GI87082152, Length=207, Percent_Identity=34.2995169082126, Blast_Score=107, Evalue=3e-24,
Organism=Escherichia coli, GI87081872, Length=295, Percent_Identity=27.4576271186441, Blast_Score=102, Evalue=7e-23,
Organism=Escherichia coli, GI1789828, Length=242, Percent_Identity=29.7520661157025, Blast_Score=92, Evalue=8e-20,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00989 PAS; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 70535; Mature: 70403

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFASIYRRKKAMLTLGQAA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGFNDGTYCAEGIIGTCALSL
HHHHHHHCCCCCEEEEEEECCEEEEECCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
AAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGTIALACPVEQTTAADLPLTLA
HHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEECCCCCCCCCCCHHHH
IAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVISWDEQGNLQFINAQAARVLRLDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEEEHHHEEEEEEEC
TASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFESQHQFIDAVITLKPIIETQGTSFI
CCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
LLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQTRRLIHFGRQAARSSFPVLLCGEEGVG
EEECCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCC
KALLSQAIHNESERAAGPYIAVNCELYGDAALAEEFIGGDRTDNENGRLSRLELAHGGTL
HHHHHHHHHCCCHHCCCCEEEEEEEECCCHHHHHHHHCCCCCCCCCCCEEEEEECCCCHH
FLEKIEYLAVELQSALLQVIKQGVITRLDARRLIPIDVKVIATTTADLAMLVEQNRFSRQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECHHHHHHHHHHHHHHHH
LYYALHAFEITIPPLRMRRGSIPALVNNKLRSLEKRFSTRLKIDDDALARLVSCAWPGND
HHHHHHHEEEECCHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCC
FELYSVIENLALSSDNGRIRVSDLPEHLFTEQATDDVSATRLSTSLSFAEVEKEAIINAA
HHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHH
QVTGGRIQEMSALLGIGRTTLWRKMKQHGIDAGQFKRRV
HHCCCHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHCCC
>Mature Secondary Structure 
SGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFASIYRRKKAMLTLGQAA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGFNDGTYCAEGIIGTCALSL
HHHHHHHCCCCCEEEEEEECCEEEEECCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
AAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGTIALACPVEQTTAADLPLTLA
HHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEECCCCCCCCCCCHHHH
IAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVISWDEQGNLQFINAQAARVLRLDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEEEHHHEEEEEEEC
TASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFESQHQFIDAVITLKPIIETQGTSFI
CCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
LLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQTRRLIHFGRQAARSSFPVLLCGEEGVG
EEECCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCC
KALLSQAIHNESERAAGPYIAVNCELYGDAALAEEFIGGDRTDNENGRLSRLELAHGGTL
HHHHHHHHHCCCHHCCCCEEEEEEEECCCHHHHHHHHCCCCCCCCCCCEEEEEECCCCHH
FLEKIEYLAVELQSALLQVIKQGVITRLDARRLIPIDVKVIATTTADLAMLVEQNRFSRQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECHHHHHHHHHHHHHHHH
LYYALHAFEITIPPLRMRRGSIPALVNNKLRSLEKRFSTRLKIDDDALARLVSCAWPGND
HHHHHHHEEEECCHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCC
FELYSVIENLALSSDNGRIRVSDLPEHLFTEQATDDVSATRLSTSLSFAEVEKEAIINAA
HHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHH
QVTGGRIQEMSALLGIGRTTLWRKMKQHGIDAGQFKRRV
HHCCCHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]