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The map label for this gene is purB

Identifier: 157160655

GI number: 157160655

Start: 1253035

End: 1254405

Strand: Reverse

Name: purB

Synonym: EcHS_A1251

Alternate gene names: 157160655

Gene position: 1254405-1253035 (Counterclockwise)

Preceding gene: 157160656

Following gene: 157160654

Centisome position: 27.01

GC content: 52.3

Gene sequence:

>1371_bases
ATGGAATTATCCTCACTGACCGCCGTTTCCCCTGTCGATGGACGCTACGGCGATAAAGTCAGCGCGCTGCGCGGGATTTT
CAGCGAATATGGTTTGCTGAAATTCCGTGTACAAGTTGAAGTACGTTGGCTGCAAAAACTGGCCGCGCACGCAGCGATCA
AGGAAGTTCCTGCTTTTGCTGCCGACGCAATCGGTTACCTTGATGCAATCGTCGCCAGTTTCAGCGAAGAAGATGCGGCG
CGCATCAAAACTATCGAGCGTACCACTAACCACGACGTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGAT
CCCGGAACTGCACGCGGTTTCTGAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCACGCATTAA
TGCTGAAAACCGCGCGTGATGAAGTGATCCTGCCATACTGGCGTCAACTGATTGATGGCATTAAAGATCTCGCCGTTCAG
TATCGCGATATCCCGCTGCTGTCTCGTACCCACGGTCAGCCAGCCACGCCGTCAACCATCGGTAAAGAGATGGCAAACGT
CGCCTACCGTATGGAGCGCCAGTACCGCCAGCTTAACCAGGTGGAGATCCTCGGCAAAATCAACGGCGCGGTCGGTAACT
ATAACGCCCACATCGCCGCTTACCCGGAAGTTGACTGGCATCAGTTCAGCGAAGAGTTCGTCACCTCGCTGGGTATTCAG
TGGAACCCGTACACCACCCAGATCGAACCGCACGACTACATTGCCGAACTGTTTGATTGCGTTGCGCGCTTCAACACTAT
TCTGATCGACTTTGACCGTGACGTCTGGGGTTATATCGCCCTTAACCACTTCAAACAGAAAACCATTGCTGGTGAGATTG
GTTCTTCCACCATGCCGCATAAAGTTAACCCGATCGACTTCGAAAACTCCGAAGGGAATCTGGGCCTTTCCAACGCGGTA
TTGCAGCATCTGGCAAGCAAACTGCCGGTTTCCCGCTGGCAGCGTGACCTGACCGACTCTACCGTGCTGCGTAACCTCGG
CGTGGGTATCGGTTATGCCTTGATTGCATATCAATCCACCCTGAAAGGCGTGAGCAAACTGGAAGTGAACCGTGACCATC
TGCTGGATGAACTGGATCACAACTGGGAAGTGCTGGCTGAACCAATCCAGACAGTTATGCGTCGCTATGGCATCGAAAAA
CCGTACGAGAAGCTGAAAGAGCTGACTCGCGGTAAGCGCGTTGACGCCGAAGGCATGAAGCAGTTTATCGATGGTCTGGC
GTTGCCAGAAGAAGAGAAAGCCCGCCTGAAAGCGATGACGCCGGCTAACTATATTGGTCGAGCTATCACGATGGTTGATG
AGCTGAAATAA

Upstream 100 bases:

>100_bases
CCAGGTCGGCGGCGGACGTCTGCAACTGATGTTTTCTCGTAATCGCCTGACCACTCAGGCAAAACAAATTCTTGCTCATT
TAACCCCGGAGTTGTGATCT

Downstream 100 bases:

>100_bases
ACCTCGTATCAGTGCCGGATGGCGATGCTGTCCGGCCTGCTTATTAAGATTATCCGCTTTTTATTTTTTCACTTTACCTC
CCCTCCCCGCTGGTTTATTT

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWLQKLAAHAAIKEVPAFAADAIGYLDAIVASFSEEDAA
RIKTIERTTNHDVKAVEYFLKEKVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHIAAYPEVDWHQFSEEFVTSLGIQ
WNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVWGYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAV
LQHLASKLPVSRWQRDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTVMRRYGIEK
PYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAITMVDELK

Sequences:

>Translated_456_residues
MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWLQKLAAHAAIKEVPAFAADAIGYLDAIVASFSEEDAA
RIKTIERTTNHDVKAVEYFLKEKVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHIAAYPEVDWHQFSEEFVTSLGIQ
WNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVWGYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAV
LQHLASKLPVSRWQRDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTVMRRYGIEK
PYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAITMVDELK
>Mature_456_residues
MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWLQKLAAHAAIKEVPAFAADAIGYLDAIVASFSEEDAA
RIKTIERTTNHDVKAVEYFLKEKVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHIAAYPEVDWHQFSEEFVTSLGIQ
WNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVWGYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAV
LQHLASKLPVSRWQRDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTVMRRYGIEK
PYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAITMVDELK

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily

Homologues:

Organism=Escherichia coli, GI1787376, Length=456, Percent_Identity=100, Blast_Score=944, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PUR8_ECOL6 (P0AB90)

Other databases:

- EMBL:   AE014075
- RefSeq:   NP_753419.1
- ProteinModelPortal:   P0AB90
- SMR:   P0AB90
- MINT:   MINT-1253726
- EnsemblBacteria:   EBESCT00000045404
- GeneID:   1035246
- GenomeReviews:   AE014075_GR
- KEGG:   ecc:c1510
- GeneTree:   EBGT00050000009426
- HOGENOM:   HBG290028
- OMA:   KVSEFIH
- ProtClustDB:   PRK09285
- BRENDA:   4.3.2.2
- InterPro:   IPR013539
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769
- PRINTS:   PR00149
- TIGRFAMs:   TIGR00928

Pfam domain/function: PF08328 ASL_C; PF00206 Lyase_1; SSF48557 L-Aspartase-like

EC number: =4.3.2.2

Molecular weight: Translated: 51543; Mature: 51543

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: ACT_SITE 91-91 ACT_SITE 171-171

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWLQKLAAHAAIKEVPAFA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHH
ADAIGYLDAIVASFSEEDAARIKTIERTTNHDVKAVEYFLKEKVAEIPELHAVSEFIHFA
HHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
CTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQYRDIPLLSRTHGQPATPSTI
CCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCHHHH
GKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHIAAYPEVDWHQFSEEFVTSLGIQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCC
WNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVWGYIALNHFKQKTIAGEIGSSTMPH
CCCCCCCCCCHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCC
KVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQRDLTDSTVLRNLGVGIGYALIAYQST
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LKGVSKLEVNRDHLLDELDHNWEVLAEPIQTVMRRYGIEKPYEKLKELTRGKRVDAEGMK
HHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHH
QFIDGLALPEEEKARLKAMTPANYIGRAITMVDELK
HHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWLQKLAAHAAIKEVPAFA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHH
ADAIGYLDAIVASFSEEDAARIKTIERTTNHDVKAVEYFLKEKVAEIPELHAVSEFIHFA
HHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
CTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQYRDIPLLSRTHGQPATPSTI
CCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCHHHH
GKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHIAAYPEVDWHQFSEEFVTSLGIQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCC
WNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVWGYIALNHFKQKTIAGEIGSSTMPH
CCCCCCCCCCHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCC
KVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQRDLTDSTVLRNLGVGIGYALIAYQST
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LKGVSKLEVNRDHLLDELDHNWEVLAEPIQTVMRRYGIEKPYEKLKELTRGKRVDAEGMK
HHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHH
QFIDGLALPEEEKARLKAMTPANYIGRAITMVDELK
HHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157