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The map label for this gene is lolE

Identifier: 157160644

GI number: 157160644

Start: 1241026

End: 1242270

Strand: Direct

Name: lolE

Synonym: EcHS_A1240

Alternate gene names: 157160644

Gene position: 1241026-1242270 (Clockwise)

Preceding gene: 157160643

Following gene: 157160645

Centisome position: 26.73

GC content: 51.81

Gene sequence:

>1245_bases
ATGGCGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCATGGTGTCGCTGATCTC
CGTCATTTCTACCATTGGCATTGCCCTCGGCGTGGCGGTATTGATCGTCGGCTTAAGCGCGATGAACGGCTTTGAACGCG
AACTGAACAACCGTATTCTGGCGGTGGTGCCGCATGGTGAAATCGAGGCGGTGGATCAGCCGTGGACTAACTGGCAGGAA
GCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGG
CGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCC
AGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGTG
AAGCAGGGGGATTGGGTGTCGATTATGATCCCCAACTCGAATCCCGAGCATAAACTGATGCAGCCAAAACGTGTGCGTTT
GCACGTTGCCGGTATTTTGCAGTTGAGTGGTCAACTCGATCACAGTTTTGCCATGATCCCGCTGGCAGATGCCCAACAAT
ATCTTGATATGGGTTCCAGCGTATCAGGTATTGCCCTTAAAATGACGGATGTTTTCAACGCCAATAAGCTGGTACGCGAT
GCGGGTGAAGTGACCAACAGCTATGTTTATATTAAAAGCTGGATTGGTACTTACGGCTATATGTATCGCGATATCCAAAT
GATCCGCGCCATTATGTATCTGGCGATGGTACTGGTGATTGGCGTGGCCTGTTTCAACATCGTCTCCACCTTAGTGATGG
CGGTGAAAGACAAGAGTGGCGATATCGCAGTATTAAGAACGCTGGGGGCGAAAGATGGTTTAATTCGCGCCATCTTTGTC
TGGTATGGATTGCTGGCAGGGCTGTTCGGCAGCCTGTGTGGTGTGATTATCGGCGTAGTGGTTTCACTGCAACTTACCCC
GATTATTGAGTGGATTGAAAAGTTGATCGGTCATCAGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGG
AATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCTTTTGGCAAGTTGGTATCCGGCGCGG
CGCGCCAGTAATATTGACCCTGCGCGAGTCCTTAGCGGCCAGTAA

Upstream 100 bases:

>100_bases
TGGTTACTCACGACCTGCAACTGGCGAAACGTATGAGCCGCCAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTG
AGCCTGATGGGGGCGGAGTA

Downstream 100 bases:

>100_bases
AGGCAGTACATTAAAACAAGGAGCGGCAATGTATTACGGGTTTGATATTGGTGGAACAAAAATTGCGCTTGGCGTGTTTG
ATAGCGGTCGGCAGTTGCAG

Product: outer membrane-specific lipoprotein transporter subunit LolE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 414; Mature: 413

Protein sequence:

>414_residues
MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVGLSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQE
ALDHVQKVPGIAAAAPYINFTGLVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMGSSVSGIALKMTDVFNANKLVRD
AGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAMVLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFV
WYGLLAGLFGSLCGVIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSLLASWYPAR
RASNIDPARVLSGQ

Sequences:

>Translated_414_residues
MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVGLSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQE
ALDHVQKVPGIAAAAPYINFTGLVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMGSSVSGIALKMTDVFNANKLVRD
AGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAMVLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFV
WYGLLAGLFGSLCGVIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSLLASWYPAR
RASNIDPARVLSGQ
>Mature_413_residues
AMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVGLSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEA
LDHVQKVPGIAAAAPYINFTGLVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKVK
QGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMGSSVSGIALKMTDVFNANKLVRDA
GEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAMVLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVW
YGLLAGLFGSLCGVIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSLLASWYPARR
ASNIDPARVLSGQ

Specific function: Part of an ATP-dependent transport system lolCDE responsible for the release of lipoproteins targeted to the outer membrane from the inner membrane. Such a release is dependent of the sorting-signal (absence of an Asp at position 2 of the mature lipoprote

COG id: COG4591

COG function: function code M; ABC-type transport system, involved in lipoprotein release, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ABC-4 integral membrane protein family. LolC/E subfamily

Homologues:

Organism=Escherichia coli, GI1787362, Length=414, Percent_Identity=100, Blast_Score=836, Evalue=0.0,
Organism=Escherichia coli, GI1787360, Length=415, Percent_Identity=26.7469879518072, Blast_Score=136, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LOLE_ECOLI (P75958)

Other databases:

- EMBL:   U00096
- EMBL:   AP009048
- PIR:   C64856
- RefSeq:   AP_001744.1
- RefSeq:   NP_415636.1
- ProteinModelPortal:   P75958
- STRING:   P75958
- EnsemblBacteria:   EBESCT00000004890
- EnsemblBacteria:   EBESCT00000017728
- GeneID:   945665
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW1104
- KEGG:   eco:b1118
- EchoBASE:   EB3215
- EcoGene:   EG13441
- eggNOG:   COG4591
- GeneTree:   EBGT00050000008253
- HOGENOM:   HBG675801
- OMA:   ANNAFIK
- ProtClustDB:   PRK11146
- BioCyc:   EcoCyc:YCFW-MONOMER
- Genevestigator:   P75958
- GO:   GO:0006810
- InterPro:   IPR003838
- InterPro:   IPR011925
- InterPro:   IPR011926
- TIGRFAMs:   TIGR02212
- TIGRFAMs:   TIGR02213

Pfam domain/function: PF02687 FtsX

EC number: NA

Molecular weight: Translated: 45345; Mature: 45214

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: PS00027 HOMEOBOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x14158654)-; HASH(0x117dd00c)-; HASH(0x13e25d5c)-; HASH(0x13d71660)-;

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVGLSAMNGFERELNNRIL
CCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCEE
AVVPHGEIEAVDQPWTNWQEALDHVQKVPGIAAAAPYINFTGLVESGANLRAIQVKGVNP
EEECCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHCCCCEEHHHHCCCCCEEEEEEECCCC
QQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKVKQGDWVSIMIPNSNPEHKLM
HHHHHHHHCHHHHCCCCCCCCCCCCCEEEEECCHHHHHHCCCCCEEEEEECCCCCCCCCC
QPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMGSSVSGIALKMTDVFNANKLVRD
CCHHHEEEHHHHHHHCCCCCCCEEEEEHHHHHHHHHCCCCCCCEEEEEHHHHCHHHHHHH
AGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAMVLVIGVACFNIVSTLVMAVKDKSG
HHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCGVIIGVVVSLQLTPIIEWIEKLIGHQF
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSSDIYFIDFLPSELHWLDVFYVLVTALLLSLLASWYPARRASNIDPARVLSGQ
HCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHCCCH
>Mature Secondary Structure 
AMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVGLSAMNGFERELNNRIL
CCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCEE
AVVPHGEIEAVDQPWTNWQEALDHVQKVPGIAAAAPYINFTGLVESGANLRAIQVKGVNP
EEECCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHCCCCEEHHHHCCCCCEEEEEEECCCC
QQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKVKQGDWVSIMIPNSNPEHKLM
HHHHHHHHCHHHHCCCCCCCCCCCCCEEEEECCHHHHHHCCCCCEEEEEECCCCCCCCCC
QPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMGSSVSGIALKMTDVFNANKLVRD
CCHHHEEEHHHHHHHCCCCCCCEEEEEHHHHHHHHHCCCCCCCEEEEEHHHHCHHHHHHH
AGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAMVLVIGVACFNIVSTLVMAVKDKSG
HHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCGVIIGVVVSLQLTPIIEWIEKLIGHQF
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSSDIYFIDFLPSELHWLDVFYVLVTALLLSLLASWYPARRASNIDPARVLSGQ
HCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10783239