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The map label for this gene is ptsG

Identifier: 157160627

GI number: 157160627

Start: 1221549

End: 1222982

Strand: Direct

Name: ptsG

Synonym: EcHS_A1223

Alternate gene names: 157160627

Gene position: 1221549-1222982 (Clockwise)

Preceding gene: 157160626

Following gene: 157160630

Centisome position: 26.31

GC content: 52.72

Gene sequence:

>1434_bases
ATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGG
TATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTT
CCGTCTTTGCAAACATGCCACTGATTTTTGCGATCGGTGTCGCCCTCGGCTTTACCAATAACGATGGCGTATCCGCGCTG
GCCGCAGTTGTTGCCTATGGCATCATGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGA
AATCGCCTCTAAACACCTGGCGGATACTGGCGTACTCGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACC
GTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATCATTTCTGGCCTGGCT
GCCATCTTTACTGGCGTTGTGCTGTCCTTCATTTGGCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGC
TTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCT
GGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTAT
ATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGC
TATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGGGCGGTATTATGATCTCCGCGGCGCTGACCTCGTTCCTGA
CCGGTATCACCGAGCCGATCGAGTTCTCCTTCATGTTCGTTGCGCCGATCCTGTACATCATCCACGCGATTCTGGCAGGC
CTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCT
GTCTGGTAACAGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCG
TGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAA
ATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGT
CAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTG
GTGTTCAGGCGATTTTCGGTACTAAATCCGACAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAA

Upstream 100 bases:

>100_bases
AATAAAGGGCGCTTAGATGCCCTGTACACGGCGAGGATCTCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCAT
ACTCAGGAGCACTCTCAATT

Downstream 100 bases:

>100_bases
TCTGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGT
AATGCTGAAATTACGCGGTG

Product: glucose-specific PTS system IIBC components

Products: NA

Alternate protein names: EIICB-Glc; EII-Glc; Glucose permease IIC component; PTS system glucose-specific EIIC component; Glucose-specific phosphotransferase enzyme IIB component; PTS system glucose-specific EIIB component

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVVSHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSAL
AAVVAYGIMVKTMAVVAPLVLHLPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPFQMQIGEYTNAAGQVFHGDIPRY
MAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPENRAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAG
LAFPICILLGMRDGTSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATEDAKATGTSE
MAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVVAGSGVQAIFGTKSDNLKTEMDEYIRNH

Sequences:

>Translated_477_residues
MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVVSHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSAL
AAVVAYGIMVKTMAVVAPLVLHLPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPFQMQIGEYTNAAGQVFHGDIPRY
MAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPENRAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAG
LAFPICILLGMRDGTSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATEDAKATGTSE
MAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVVAGSGVQAIFGTKSDNLKTEMDEYIRNH
>Mature_477_residues
MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVVSHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSAL
AAVVAYGIMVKTMAVVAPLVLHLPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPFQMQIGEYTNAAGQVFHGDIPRY
MAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPENRAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAG
LAFPICILLGMRDGTSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATEDAKATGTSE
MAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVVAGSGVQAIFGTKSDNLKTEMDEYIRNH

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain

Homologues:

Organism=Escherichia coli, GI1787343, Length=477, Percent_Identity=100, Blast_Score=959, Evalue=0.0,
Organism=Escherichia coli, GI1786894, Length=485, Percent_Identity=40.2061855670103, Blast_Score=334, Evalue=7e-93,
Organism=Escherichia coli, GI1787908, Length=516, Percent_Identity=36.8217054263566, Blast_Score=268, Evalue=7e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTGCB_ECO57 (P69788)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   G90813
- RefSeq:   NP_287235.1
- RefSeq:   NP_309506.1
- ProteinModelPortal:   P69788
- SMR:   P69788
- MINT:   MINT-264968
- PRIDE:   P69788
- EnsemblBacteria:   EBESCT00000025323
- EnsemblBacteria:   EBESCT00000060061
- GeneID:   912363
- GeneID:   959449
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z1740
- KEGG:   ecs:ECs1479
- GeneTree:   EBGT00050000009159
- HOGENOM:   HBG571563
- OMA:   FSDWAAH
- ProtClustDB:   PRK11089
- BioCyc:   ECOL83334:ECS1479-MONOMER
- InterPro:   IPR018113
- InterPro:   IPR004719
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR011299
- Gene3D:   G3DSA:3.30.1360.60
- TIGRFAMs:   TIGR00826
- TIGRFAMs:   TIGR00852
- TIGRFAMs:   TIGR02002

Pfam domain/function: PF00367 PTS_EIIB; PF02378 PTS_EIIC; SSF55604 PTS_EIIB

EC number: =2.7.1.69

Molecular weight: Translated: 50677; Mature: 50677

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: PS51098 PTS_EIIB_TYPE_1; PS01035 PTS_EIIB_TYPE_1_CYS; PS51103 PTS_EIIC_TYPE_1

Important sites: ACT_SITE 421-421

Signals:

None

Transmembrane regions:

HASH(0x12fc50c4)-; HASH(0x142fd62c)-; HASH(0x141f4b40)-; HASH(0x138654c8)-; HASH(0x1304ce1c)-; HASH(0x13163960)-; HASH(0x1431aa04)-; HASH(0x134dc06c)-; HASH(0x13dcafe8)-; HASH(0x11bdf7d4)-;

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVVSHVMAEAGGSVFANMP
CCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEECCCH
LIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLHLPAEEIASKHLADTGVLG
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
GIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLAAIFTGVVLSFIWPPIGSAIQ
HHHHHHHHHHHHHHHHEEECHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
TFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPFQMQIGEYTNAAGQVFHGDIPRY
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCEECCCCCH
MAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPENRAKVGGIMISAALTSFLTGITEPI
HCCCCCCCCCCCCCEEEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCHH
EFSFMFVAPILYIIHAILAGLAFPICILLGMRDGTSFSHGLIDFIVLSGNSSKLWLFPIV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCEEEEEHHH
GIGYAIVYYTIFRVLIKALDLKTPGREDATEDAKATGTSEMAPALVAAFGGKENITNLDA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHCCCCCCCHHHH
CITRLRVSVADVSKVDQAGLKKLGAAGVVVAGSGVQAIFGTKSDNLKTEMDEYIRNH
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEECCCCCCHHHHHHHHHHCH
>Mature Secondary Structure
MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVVSHVMAEAGGSVFANMP
CCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEECCCH
LIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLHLPAEEIASKHLADTGVLG
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
GIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLAAIFTGVVLSFIWPPIGSAIQ
HHHHHHHHHHHHHHHHEEECHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
TFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPFQMQIGEYTNAAGQVFHGDIPRY
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCEECCCCCH
MAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPENRAKVGGIMISAALTSFLTGITEPI
HCCCCCCCCCCCCCEEEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCHH
EFSFMFVAPILYIIHAILAGLAFPICILLGMRDGTSFSHGLIDFIVLSGNSSKLWLFPIV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCEEEEEHHH
GIGYAIVYYTIFRVLIKALDLKTPGREDATEDAKATGTSEMAPALVAAFGGKENITNLDA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHCCCCCCCHHHH
CITRLRVSVADVSKVDQAGLKKLGAAGVVVAGSGVQAIFGTKSDNLKTEMDEYIRNH
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEECCCCCCHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796