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The map label for this gene is insA [H]

Identifier: 157160549

GI number: 157160549

Start: 1153678

End: 1153953

Strand: Reverse

Name: insA [H]

Synonym: EcHS_A1141

Alternate gene names: 157160549

Gene position: 1153953-1153678 (Counterclockwise)

Preceding gene: 157160551

Following gene: 157160548

Centisome position: 24.85

GC content: 52.9

Gene sequence:

>276_bases
GTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACCGCCGGACA
TCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACTGCAGTTCACTTACACCGCTTCTCAACCCGGTACGCACC
AGAAAATCATTGATATGGCCATGAATGGCGTTGGATGCCGGGCAACTGCCCGCATTATGGGCGTTGGCCTCAACACGATT
TTACGTCACTTAAAAAACTCAGGCCGCAGTCGGTAA

Upstream 100 bases:

>100_bases
GCATTGATTACGCGAGTGTGTTACAAGCCCGCGGTTGGCCTCGTGGGTGATGCTGCCAACTTACTGATTTAGTGTATGAT
GGTGTTTTTGAGGTGCTCCA

Downstream 100 bases:

>100_bases
CCTCGCGCATACAGCCGGGCAGTGACGTCATCGTCTGCGCGGAAATGGACGAACAGTGGGGCTATGTCGGGGCTAAATCG
CGCCAGCGCTGGCTGTTTTA

Product: IS1, transposase orfA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 91; Mature: 90

Protein sequence:

>91_residues
MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFTYTASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTI
LRHLKNSGRSR

Sequences:

>Translated_91_residues
MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFTYTASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTI
LRHLKNSGRSR
>Mature_90_residues
ASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFTYTASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTIL
RHLKNSGRSR

Specific function: Absolutely required for transposition of IS1 [H]

COG id: COG3677

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IS1 elements insA family [H]

Homologues:

Organism=Escherichia coli, GI1789852, Length=91, Percent_Identity=100, Blast_Score=189, Evalue=2e-50,
Organism=Escherichia coli, GI1788204, Length=91, Percent_Identity=100, Blast_Score=189, Evalue=2e-50,
Organism=Escherichia coli, GI1786204, Length=91, Percent_Identity=100, Blast_Score=189, Evalue=2e-50,
Organism=Escherichia coli, GI87081814, Length=91, Percent_Identity=98.9010989010989, Blast_Score=188, Evalue=6e-50,
Organism=Escherichia coli, GI1786471, Length=91, Percent_Identity=98.9010989010989, Blast_Score=188, Evalue=6e-50,
Organism=Escherichia coli, GI1786460, Length=91, Percent_Identity=98.9010989010989, Blast_Score=188, Evalue=6e-50,
Organism=Escherichia coli, GI1790747, Length=91, Percent_Identity=92.3076923076923, Blast_Score=178, Evalue=5e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012287
- InterPro:   IPR003220 [H]

Pfam domain/function: PF03811 Ins_element1 [H]

EC number: NA

Molecular weight: Translated: 9869; Mature: 9737

Theoretical pI: Translated: 10.86; Mature: 10.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.5 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
9.9 %Cys+Met (Translated Protein)
5.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
8.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFTYTASQPGTHQKIIDMA
CCCCCCCCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHCEEEEEEEECCCCCCHHHHHHHH
MNGVGCRATARIMGVGLNTILRHLKNSGRSR
HCCCCCHHHHHHHHCCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFTYTASQPGTHQKIIDMA
CCCCCCCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHCEEEEEEEECCCCCCHHHHHHHH
MNGVGCRATARIMGVGLNTILRHLKNSGRSR
HCCCCCHHHHHHHHCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6265806 [H]