Click here to switch to the map view.

The map label for this gene is appB

Identifier: 157160498

GI number: 157160498

Start: 1100910

End: 1102046

Strand: Direct

Name: appB

Synonym: EcHS_A1087

Alternate gene names: 157160498

Gene position: 1100910-1102046 (Clockwise)

Preceding gene: 157160497

Following gene: 157160499

Centisome position: 23.71

GC content: 53.21

Gene sequence:

>1137_bases
ATGTTTGATTATGAAACATTGCGCTTCATCTGGTGGCTGCTGATTGGCGTGATCCTGGTGGTCTTTATGATCTCCGACGG
ATTTGACATGGGGATCGGCTGTCTGCTGCCGCTGGTGGCGCGTAATGATGATGAACGCCGGATAGTGATAAACAGCGTTG
GTGCACACTGGGAAGGCAACCAGGTCTGGTTGATCCTCGCTGGTGGGGCATTATTTGCCGCCTGGCCCAGAGTGTATGCA
GCGGCGTTTTCCGGCTTTTATGTGGCGATGATCCTGGTGCTGTGCTCACTGTTCTTCCGCCCGCTGGCCTTTGATTATCG
CGGAAAAATCGCCGATGCACGCTGGCGTAAAATGTGGGACGCCGGTCTGGTCATCGGCAGTCTGGTGCCGCCGGTAGTCT
TCGGTATCGCCTTCGGCAACTTGTTGCTCGGCGTGCCGTTTGCCTTCACACCGCAATTACGCGTGGAGTATCTCGGCAGC
TTCTGGCAACTGCTGACGCCATTCCCTTTATTGTGCGGATTGCTCAGCCTTGGGATGGTGATTTTGCAAGGTGGCGTCTG
GTTACAACTGAAAACTGTTGGTGTGATTCATCTGCGTTCACAGCTGGCCACCAAACGCGCTGCACTGTTGGTGATGCTGT
GCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCC
AATCCGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTCGTCGAATCGCCCGTTTTGTGGATCTT
CCCGCTGCTGGGGTTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTAATGG
CGTCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCA
ATCTCCAGCCTGACGTTGTGGGACAGCACTTCCAGTCAGCTGACGCTGAGCATTATGTTGGTAATCGTGCTGATATTTTT
GCCCATTGTGTTGCTCTACACTCTCTGGAGCTACTACAAAATGTGGGGACGCATGACAACAGAAACTCTCCGCCGTAACG
AAAACGAGTTGTACTAA

Upstream 100 bases:

>100_bases
TAATCGCCGAAGTCTACCTGATGCAGAAATATGCCCGTCTGGGGCCGAGCGCGATGCAGAGTGAACAACCGACGCAGCAA
CAGGGGTAAAGGAGAAAATC

Downstream 100 bases:

>100_bases
GGAGCAGGAACAATGTGGTATTTACTTTGGTTCGTCGGCATTTTGTTGATGTGTTCGCTCTCCACCCTTGTGTTGGTATG
GCTGGACCCGCGTCTGAAAA

Product: cytochrome bd-II oxidase, subunit II

Products: NA

Alternate protein names: Cytochrome bd-II oxidase subunit II

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDDERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYA
AAFSGFYVAMILVLCSLFFRPLAFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLAGYWLWVGIDGFVLLAQDANGPS
NPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPLLTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSP
ISSLTLWDSTSSQLTLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY

Sequences:

>Translated_378_residues
MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDDERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYA
AAFSGFYVAMILVLCSLFFRPLAFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLAGYWLWVGIDGFVLLAQDANGPS
NPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPLLTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSP
ISSLTLWDSTSSQLTLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY
>Mature_378_residues
MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDDERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYA
AAFSGFYVAMILVLCSLFFRPLAFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLAGYWLWVGIDGFVLLAQDANGPS
NPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPLLTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSP
ISSLTLWDSTSSQLTLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY

Specific function: Cytochrome oxidase subunit

COG id: COG1294

COG function: function code C; Cytochrome bd-type quinol oxidase, subunit 2

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To E.coli CydB

Homologues:

Organism=Escherichia coli, GI1787213, Length=378, Percent_Identity=100, Blast_Score=747, Evalue=0.0,
Organism=Escherichia coli, GI1786954, Length=379, Percent_Identity=57.5197889182058, Blast_Score=450, Evalue=1e-128,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): APPB_ECOLI (P26458)

Other databases:

- EMBL:   S63811
- EMBL:   U00096
- EMBL:   AP009048
- RefSeq:   AP_001608.1
- RefSeq:   NP_415498.1
- ProteinModelPortal:   P26458
- STRING:   P26458
- EnsemblBacteria:   EBESCT00000001080
- EnsemblBacteria:   EBESCT00000015848
- GeneID:   947547
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW0961
- KEGG:   eco:b0979
- EchoBASE:   EB1353
- EcoGene:   EG11379
- eggNOG:   COG1294
- GeneTree:   EBGT00050000009321
- HOGENOM:   HBG413915
- OMA:   ATAIFLP
- ProtClustDB:   PRK15028
- BioCyc:   EcoCyc:APPB-MONOMER
- BioCyc:   MetaCyc:APPB-MONOMER
- Genevestigator:   P26458
- GO:   GO:0006810
- InterPro:   IPR003317
- TIGRFAMs:   TIGR00203

Pfam domain/function: PF02322 Cyto_ox_2

EC number: 1.10.3.-

Molecular weight: Translated: 42424; Mature: 42424

Theoretical pI: Translated: 8.68; Mature: 8.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x124e63b4)-; HASH(0x13917198)-; HASH(0x139e21c0)-; HASH(0x13ec3058)-; HASH(0x13efeb40)-; HASH(0x1379ff64)-; HASH(0x12b8b42c)-; HASH(0x13e80194)-;

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDDERRIVINSVGAHWEGN
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCC
QVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPLAFDYRGKIADARWRKMWD
EEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
AGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGSFWQLLTPFPLLCGLLSLGMV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
ILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLAGYWLWVGIDGFVLLAQDANGPS
HHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCC
NPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPLLTVMAIYRGRPGWGFLMASLMQFG
HHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
VIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQLTLSIMLVIVLIFLPIVLLYTLWSYYK
HHHHHHHHHHHHHCCCCCCCCHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MWGRMTTETLRRNENELY
HHHHHHHHHHHCCCCCCH
>Mature Secondary Structure
MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDDERRIVINSVGAHWEGN
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCC
QVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPLAFDYRGKIADARWRKMWD
EEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
AGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGSFWQLLTPFPLLCGLLSLGMV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
ILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLAGYWLWVGIDGFVLLAQDANGPS
HHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCC
NPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPLLTVMAIYRGRPGWGFLMASLMQFG
HHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
VIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQLTLSIMLVIVLIFLPIVLLYTLWSYYK
HHHHHHHHHHHHHCCCCCCCCHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MWGRMTTETLRRNENELY
HHHHHHHHHHHCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Oxidoreductases; Acting on diphenols and related substances as donors; With oxygen as acceptor [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1658595; 8905232; 9278503; 8626304