Click here to switch to the map view.

The map label for this gene is tusE

Identifier: 157160489

GI number: 157160489

Start: 1091953

End: 1092282

Strand: Reverse

Name: tusE

Synonym: EcHS_A1077

Alternate gene names: 157160489

Gene position: 1092282-1091953 (Counterclockwise)

Preceding gene: 157160490

Following gene: 157160488

Centisome position: 23.52

GC content: 46.67

Gene sequence:

>330_bases
ATGCTGATCTTCGAAGGTAAAGAGATAGAAACGGATACCGAAGGCTATCTCAAAGAAAGCAGCCAGTGGAGTGAGCCGCT
GGCTGTGGTGATTGCAGAGAACGAAGGGATTTCGCTGTCACCGGAACACTGGGAAGTGGTGCGTTTTGTGCGTGATTTCT
ATCTGGAATTCAATACTTCTCCGGCGATTCGTATGCTGGTAAAAGCGATGGCGAATAAATTTGGCGAAGAAAAAGGTAAC
AGCCGCTATTTATACCGACTGTTTCCGAAAGGTCCGGCTAAGCAAGCCACCAAAATTGCTGGCCTGCCAAAACCGGTAAA
ATGTATTTAA

Upstream 100 bases:

>100_bases
CCGCGATTAATCTCACCCGCTAACACTTTCATAGCCTCGCTTTATGCGGGGCTTTGTTTTTTGTTACACTGCCGCCAGTC
TGATTAAGGTAGTAATAATT

Downstream 100 bases:

>100_bases
CGGATACGAAAATCGGTTAATTCCCCCGAGGGATGATGCGGCTCGCTGAGCACCCGTTCTACACGCGCAGAACGCGGGCC
GCCACTCTTTAACCACTGCA

Product: sulfur transfer protein TusE

Products: NA

Alternate protein names: tRNA 2-thiouridine synthesizing protein E

Number of amino acids: Translated: 109; Mature: 109

Protein sequence:

>109_residues
MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHWEVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGN
SRYLYRLFPKGPAKQATKIAGLPKPVKCI

Sequences:

>Translated_109_residues
MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHWEVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGN
SRYLYRLFPKGPAKQATKIAGLPKPVKCI
>Mature_109_residues
MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHWEVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGN
SRYLYRLFPKGPAKQATKIAGLPKPVKCI

Specific function: Part of a sulfur-relay system required for 2-thiolation of 5-methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at tRNA wobble positions. Could accept sulfur from tusD

COG id: COG2920

COG function: function code P; Dissimilatory sulfite reductase (desulfoviridin), gamma subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dsrC/tusE family

Homologues:

Organism=Escherichia coli, GI87081811, Length=109, Percent_Identity=100, Blast_Score=225, Evalue=5e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TUSE_ECO57 (Q8XD82)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   C85624
- PIR:   E90760
- RefSeq:   NP_286844.2
- RefSeq:   NP_309080.2
- ProteinModelPortal:   Q8XD82
- SMR:   Q8XD82
- EnsemblBacteria:   EBESCT00000024326
- EnsemblBacteria:   EBESCT00000058375
- GeneID:   912999
- GeneID:   958965
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z1321
- KEGG:   ecs:ECs1053
- GeneTree:   EBGT00050000010194
- HOGENOM:   HBG739529
- OMA:   YQIAPAV
- ProtClustDB:   PRK11508
- BioCyc:   ECOL83334:ECS1053-MONOMER
- GO:   GO:0005737
- InterPro:   IPR007453
- Gene3D:   G3DSA:1.10.10.370
- TIGRFAMs:   TIGR03342

Pfam domain/function: PF04358 DsrC; SSF69721 DsrC

EC number: NA

Molecular weight: Translated: 12410; Mature: 12410

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: NA

Important sites: ACT_SITE 108-108

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHWEVVRFVRDFYLEFNTS
CEEECCCCCCCCCCHHHHCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCC
PAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGLPKPVKCI
HHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHWEVVRFVRDFYLEFNTS
CEEECCCCCCCCCCHHHHCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCC
PAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGLPKPVKCI
HHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796