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The map label for this gene is helD

Identifier: 157160483

GI number: 157160483

Start: 1086085

End: 1088139

Strand: Direct

Name: helD

Synonym: EcHS_A1071

Alternate gene names: 157160483

Gene position: 1086085-1088139 (Clockwise)

Preceding gene: 157160480

Following gene: 157160486

Centisome position: 23.39

GC content: 52.94

Gene sequence:

>2055_bases
ATGGAACTGAAAGCGACAACGCTTGGAAAACGTCTGGCACAGCACCCTTACGATCGGGCGGTGATCCTCAATGCCGGGAT
TAAAGTCTCCGGCGATCGCCACGAATACCTTATTCCTTTCAATCAATTACTGGCGATTCACTGTAAGCGCGGTCTGGTAT
GGGGCGAGCTGGAATTTGTACTGCCGGACGAAAAAGTGGTGCGTCTGCACGGCACCGAATGGGGCGAGACGCAGCGTTTT
TACCATCATCTTGATGCTCACTGGCGGCGGTGGAGTGGCGAGATGAGCGAAATTGCGTCTGGTGTTTTACGCCAGCAACT
GGATTTGATTGCCACGCGCACTGGAGAAAATAAATGGCTGACGCGTGAGCAAACCTCTGGCGTTCAGCAACAAATCCGCC
AGGCTTTGTCGGCGTTGCCGTTGCCGGTTAACCGACTGGAAGAATTCGATAACTGCCGTGAGGCGTGGCGTAAATGTCAG
GCCTGGTTGAAAGATATTGAAAGCGCTCGGTTGCAGCATAACCAGGCGTATACCGAAGCCATGCTTACCGAGTATGCGGA
TTTTTTCCGCCAGGTCGAGTCTTCACCGCTGAATCCGGCGCAGGCCCGGGCAGTCGTTAATGGCGAGCATTCTCTGTTAG
TGCTGGCAGGTGCAGGAAGCGGAAAAACGTCGGTGCTGGTGGCCCGTGCAGGCTGGTTGCTGGCGCGTGGTGAAGCGTCC
CCTGAGCAAATTTTATTGCTGGCGTTTGGTCGCAAAGCCGCTGAAGAGATGGACGAGCGGATTCGCGAACGGCTACATAC
CGAAGACATTACCGCACGCACGTTTCATGCGCTGGCGCTGCATATTATTCAGCAGGGCAGCAAAAAAGTTCCGATAGTCA
GCAAACTGGAAAATGATACCGCTGCCCGTCATGAACTCTTTATTGCTGAGTGGCGCAAGCAATGCAGCGAAAAGAAAGCG
CAGGCGAAGGGCTGGCGGCAATGGCTGACGGAAGAAATGCAGTGGTCAGTGCCAGAAGGTAACTTCTGGGATGATGAAAA
ATTACAGCGTCGCCTTGCCTCTCGCCTCGATCGCTGGGTAAGTCTGATGCGGATGCACGGTGGTGCACAGGCAGAAATGA
TTGCCAGTGCACCCGAAGAGATTCGCGATCTGTTCAGTAAACGTATCAAGTTGATGGCCCCGTTATTAAAAGCCTGGAAA
GGTGCGCTGAAGGCAGAAAACGCTGTCGATTTTTCGGGCCTTATTCATCAGGCGATTGTGATTCTGGAGAAAGGTCGCTT
TATCAGCCCGTGGAAGCATATTCTGGTTGATGAATTTCAGGATATCTCGCCGCAGCGGGCAGCGTTGTTAGCGGCATTAC
GCAAGCAAAACAGTCAGACGACGTTGTTCGCTGTTGGTGATGACTGGCAGGCGATTTACCGATTCAGCGGTGCGCAAATG
TCGCTCACCACCGCTTTCCATGAAAACTTTGGTGAAGGCGAACGCTGTGATTTAGACACGACTTACCGTTTTAACAGTCG
TATCGGTGAGGTGGCAAACCGGTTTATTCAGCAGAACCCAGGCCAGCTGAAAAAGCCGCTAAACAGCTTAACCAATGGAG
ACAAAAAAGCCGTCACGTTATTGGATGAGAGTCAACTTGACGCTTTGCTGGATAAGCTCTCTGGTTATGCCAAACCGGAA
GAGCGCATTCTGATCCTGGCGCGTTACCATCACATGAGGCCTGCCAGCCTGGAAAAAGCGGCAACACGCTGGCCGAAGTT
GCAAATCGACTTTATGACCATTCATGCCAGCAAAGGGCAACAGGCGGATTACGTCATCATCGTTGGCTTGCAGGAGGGAA
GTGATGGTTTTCCGGCTGCGGCGCGGGAGTCGATTATGGAAGAGGCGCTACTGCCACCGGTTGAGGATTTCCCGGACGCT
GAAGAACGGCGGTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTC
TCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAA

Upstream 100 bases:

>100_bases
TTCAGTAATGGGCGGCAATTGTAACGTTTTTTTGGGCTGGAGCACGTTTTCTCTGACGGTTACACTGATAAGAAATAATT
TCGTGTGGATCTACAGAGTC

Downstream 100 bases:

>100_bases
GAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATA
TCGACCGCGTCGTTGAAATG

Product: DNA helicase IV

Products: NA

Alternate protein names: 75 kDa helicase

Number of amino acids: Translated: 684; Mature: 684

Protein sequence:

>684_residues
MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLLAIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRF
YHHLDAHWRRWSGEMSEIASGVLRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGAGSGKTSVLVARAGWLLARGEAS
PEQILLLAFGRKAAEEMDERIRERLHTEDITARTFHALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKA
QAKGWRQWLTEEMQWSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLMAPLLKAWK
GALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALLAALRKQNSQTTLFAVGDDWQAIYRFSGAQM
SLTTAFHENFGEGERCDLDTTYRFNSRIGEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPE
ERILILARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARESIMEEALLPPVEDFPDA
EERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVARKP

Sequences:

>Translated_684_residues
MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLLAIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRF
YHHLDAHWRRWSGEMSEIASGVLRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGAGSGKTSVLVARAGWLLARGEAS
PEQILLLAFGRKAAEEMDERIRERLHTEDITARTFHALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKA
QAKGWRQWLTEEMQWSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLMAPLLKAWK
GALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALLAALRKQNSQTTLFAVGDDWQAIYRFSGAQM
SLTTAFHENFGEGERCDLDTTYRFNSRIGEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPE
ERILILARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARESIMEEALLPPVEDFPDA
EERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVARKP
>Mature_684_residues
MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLLAIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRF
YHHLDAHWRRWSGEMSEIASGVLRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGAGSGKTSVLVARAGWLLARGEAS
PEQILLLAFGRKAAEEMDERIRERLHTEDITARTFHALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKA
QAKGWRQWLTEEMQWSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLMAPLLKAWK
GALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALLAALRKQNSQTTLFAVGDDWQAIYRFSGAQM
SLTTAFHENFGEGERCDLDTTYRFNSRIGEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPE
ERILILARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARESIMEEALLPPVEDFPDA
EERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVARKP

Specific function: Helicase IV catalyzes the unwinding of duplex DNA in the 3' to 5' direction with respect to the bound single strand in a reaction that is dependent upon the hydrolysis of ATP

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase ATP-binding domain

Homologues:

Organism=Escherichia coli, GI1787196, Length=684, Percent_Identity=100, Blast_Score=1406, Evalue=0.0,
Organism=Escherichia coli, GI2367296, Length=340, Percent_Identity=28.5294117647059, Blast_Score=100, Evalue=6e-22,
Organism=Escherichia coli, GI48994965, Length=341, Percent_Identity=28.1524926686217, Blast_Score=94, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6322369, Length=352, Percent_Identity=28.125, Blast_Score=95, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HELD_ECOLI (P15038)

Other databases:

- EMBL:   J04726
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   A64837
- RefSeq:   AP_001592.1
- RefSeq:   NP_415482.1
- ProteinModelPortal:   P15038
- SMR:   P15038
- DIP:   DIP-9876N
- IntAct:   P15038
- MINT:   MINT-1226516
- STRING:   P15038
- EnsemblBacteria:   EBESCT00000002172
- EnsemblBacteria:   EBESCT00000018217
- GeneID:   946227
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW0945
- KEGG:   eco:b0962
- EchoBASE:   EB0421
- EcoGene:   EG10426
- eggNOG:   COG0210
- GeneTree:   EBGT00050000008889
- HOGENOM:   HBG303431
- OMA:   LAQHPYN
- ProtClustDB:   PRK11054
- BioCyc:   EcoCyc:EG10426-MONOMER
- Genevestigator:   P15038
- GO:   GO:0016020
- InterPro:   IPR000212
- InterPro:   IPR014016
- InterPro:   IPR022161
- InterPro:   IPR003661
- PANTHER:   PTHR11070
- SMART:   SM00388

Pfam domain/function: PF12462 Nucleolin_N; PF00580 UvrD-helicase

EC number: =3.6.4.12

Molecular weight: Translated: 77977; Mature: 77977

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: PS51198 UVRD_HELICASE_ATP_BIND

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLLAIHCKRGLVWGELEFV
CCCCHHHHHHHHHHCCCCCEEEEECCEEECCCCCEEECCHHHHHHHHHHCCCEEEEEEEE
LPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGVLRQQLDLIATRTGENKWL
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
TREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQAWLKDIESARLQHNQAYTEA
CHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGAGSGKTSVLVARAGWLLARGEAS
HHHHHHHHHHHHHCCCCCHHHHHEEECCCCEEEEEEECCCCCCEEEEEECCEEEECCCCC
PEQILLLAFGRKAAEEMDERIRERLHTEDITARTFHALALHIIQQGSKKVPIVSKLENDT
HHHEEEEHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHCCCH
AARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQWSVPEGNFWDDEKLQRRLASRLDRWV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
SLMRMHGGAQAEMIASAPEEIRDLFSKRIKLMAPLLKAWKGALKAENAVDFSGLIHQAIV
HHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
ILEKGRFISPWKHILVDEFQDISPQRAALLAALRKQNSQTTLFAVGDDWQAIYRFSGAQM
HHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHCCCCCE
SLTTAFHENFGEGERCDLDTTYRFNSRIGEVANRFIQQNPGQLKKPLNSLTNGDKKAVTL
EEHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEE
LDESQLDALLDKLSGYAKPEERILILARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQ
ECHHHHHHHHHHHCCCCCCHHHEEEEEHHCCCCCCHHHHHHHCCCCEEEEEEEEECCCCC
QADYVIIVGLQEGSDGFPAAARESIMEEALLPPVEDFPDAEERRLMYVALTRARHRVWAL
CCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHEEEHHHHHHHHHHEEEE
FNKENPSPFVEILKNLDVPVARKP
ECCCCCCHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLLAIHCKRGLVWGELEFV
CCCCHHHHHHHHHHCCCCCEEEEECCEEECCCCCEEECCHHHHHHHHHHCCCEEEEEEEE
LPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGVLRQQLDLIATRTGENKWL
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
TREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQAWLKDIESARLQHNQAYTEA
CHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGAGSGKTSVLVARAGWLLARGEAS
HHHHHHHHHHHHHCCCCCHHHHHEEECCCCEEEEEEECCCCCCEEEEEECCEEEECCCCC
PEQILLLAFGRKAAEEMDERIRERLHTEDITARTFHALALHIIQQGSKKVPIVSKLENDT
HHHEEEEHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHCCCH
AARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQWSVPEGNFWDDEKLQRRLASRLDRWV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
SLMRMHGGAQAEMIASAPEEIRDLFSKRIKLMAPLLKAWKGALKAENAVDFSGLIHQAIV
HHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
ILEKGRFISPWKHILVDEFQDISPQRAALLAALRKQNSQTTLFAVGDDWQAIYRFSGAQM
HHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHCCCCCE
SLTTAFHENFGEGERCDLDTTYRFNSRIGEVANRFIQQNPGQLKKPLNSLTNGDKKAVTL
EEHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEE
LDESQLDALLDKLSGYAKPEERILILARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQ
ECHHHHHHHHHHHCCCCCCHHHEEEEEHHCCCCCCHHHHHHHCCCCEEEEEEEEECCCCC
QADYVIIVGLQEGSDGFPAAARESIMEEALLPPVEDFPDAEERRLMYVALTRARHRVWAL
CCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHEEEHHHHHHHHHHEEEE
FNKENPSPFVEILKNLDVPVARKP
ECCCCCCHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2542273; 8905232; 9278503