Click here to switch to the map view.

The map label for this gene is ycaN

Identifier: 157160423

GI number: 157160423

Start: 1009004

End: 1009912

Strand: Reverse

Name: ycaN

Synonym: EcHS_A1006

Alternate gene names: 157160423

Gene position: 1009912-1009004 (Counterclockwise)

Preceding gene: 157160424

Following gene: 157160420

Centisome position: 21.75

GC content: 46.64

Gene sequence:

>909_bases
ATGCGGATGAATATGTCTGACTTTGCCACTTTCTTTGCCGTGGCCCGTAATCAAAGCTTTCGTGCAGCGGGCGATGAGTT
AGGCTTATCCTCGTCCGCCATTAGCCATAGTATTAAAACACTGGAACAACGTCTTAAAATTCGCTTATTCAATCGGACAA
CCCGAAGCGTATCGTTAACGGAAGCGGGTTCTAATCTCTATGAACGATTGCGCCCAGCTTTCGACGAAATTCAGATCATG
TTAGATGAGATGAATGATTTTCGTCTGACACCGACAGGAACGCTCAAAATCAATGCCGCCCGGGTAGCTGCACGTATATT
TTTAATGTCTTTACTGGTTGGTTTCACACGTGAATATCCTGATATTAAAGTCGAGCTCACAACTGACGACTCTCTTGTCG
ATATCGTCCAACAGGGCTTTGACGCTGGCGTTCGACTTAGTTGCATCGTCGAAAAAGATATGATCAGCGTGGCGATCGGC
CCGCCAGTGAAATTATGTGTAGCAGCAACGCCAGAGTACTTTGCAAGATATGGCAAACCCCGCCATCCTCATGATCTGCT
TAATCATCAATGTGTTGTTTTTCGTTATCCCAGCGGCAAACCTTTCCACTGGCAATTTGCAAAGGAGCTGGAAATTGCTG
TCGCAGGAAATATCATTCTGGATGATGTTGATGCTGAACTGGAGGCCGTTTTGATGGGTGCTGGTATCGGGTATCTGCTC
TATGAACAGATCAAGGAGTACCTTGATACTGGTCGTTTAGAATGTGTACTGGAAGACTGGAGCACAGAGCGTCCAGGCTT
CCAGATCTATTATCCAAACCGCCAGTATATGTCCTGCGGCTTGCGCGCATTTCTCGATTATGTAAAAACGGGTCAGATCT
GCCAGAGTCAGCGTCACCGACCACAATAA

Upstream 100 bases:

>100_bases
TAATTTTACCTCCACGTTAAGTTCTGGAGCGCACTTTACGCAGGCGGCATAATCCTGTAAACGTGATGATTCAGGATGAG
TTAATGAAGGATTTTCACTA

Downstream 100 bases:

>100_bases
TTATTTACTATTCGCTTTACGCGCCAGCAGCGGGAATATCAGTCCTAATCCTACCAGCACAAACGGCGTTGCAACATTCA
GCGCCAACTGGAAGGTCCAC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQRLKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIM
LDEMNDFRLTPTGTLKINAARVAARIFLMSLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSCIVEKDMISVAIG
PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNIILDDVDAELEAVLMGAGIGYLL
YEQIKEYLDTGRLECVLEDWSTERPGFQIYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ

Sequences:

>Translated_302_residues
MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQRLKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIM
LDEMNDFRLTPTGTLKINAARVAARIFLMSLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSCIVEKDMISVAIG
PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNIILDDVDAELEAVLMGAGIGYLL
YEQIKEYLDTGRLECVLEDWSTERPGFQIYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ
>Mature_302_residues
MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQRLKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIM
LDEMNDFRLTPTGTLKINAARVAARIFLMSLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSCIVEKDMISVAIG
PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNIILDDVDAELEAVLMGAGIGYLL
YEQIKEYLDTGRLECVLEDWSTERPGFQIYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1787128, Length=302, Percent_Identity=100, Blast_Score=630, Evalue=0.0,
Organism=Escherichia coli, GI1787589, Length=292, Percent_Identity=33.2191780821918, Blast_Score=200, Evalue=9e-53,
Organism=Escherichia coli, GI1786401, Length=296, Percent_Identity=28.7162162162162, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1789639, Length=304, Percent_Identity=27.3026315789474, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI87081978, Length=289, Percent_Identity=28.0276816608997, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI145693193, Length=282, Percent_Identity=28.3687943262411, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1789440, Length=298, Percent_Identity=24.1610738255034, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1788706, Length=300, Percent_Identity=27, Blast_Score=87, Evalue=9e-19,
Organism=Escherichia coli, GI1786448, Length=297, Percent_Identity=28.956228956229, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788748, Length=123, Percent_Identity=34.9593495934959, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI145693105, Length=160, Percent_Identity=28.125, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789173, Length=306, Percent_Identity=24.1830065359477, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1787601, Length=140, Percent_Identity=25.7142857142857, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): YCAN_ECOLI (P75836)

Other databases:

- EMBL:   U00096
- EMBL:   AP009048
- PIR:   C64829
- RefSeq:   AP_001530.1
- RefSeq:   NP_415420.1
- ProteinModelPortal:   P75836
- STRING:   P75836
- EnsemblBacteria:   EBESCT00000002354
- EnsemblBacteria:   EBESCT00000018343
- GeneID:   945523
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW0883
- KEGG:   eco:b0900
- EchoBASE:   EB3462
- EcoGene:   EG13698
- eggNOG:   COG0583
- GeneTree:   EBGT00070000031706
- HOGENOM:   HBG685425
- OMA:   IAVARYK
- ProtClustDB:   CLSK891723
- BioCyc:   EcoCyc:G6467-MONOMER
- Genevestigator:   P75836
- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate

EC number: NA

Molecular weight: Translated: 34295; Mature: 34295

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQRLKIRLFNRTTRSVSLT
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEECCCCCEEEEH
EAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARVAARIFLMSLLVGFTREYP
HHHHHHHHHHCCHHHHHHHHHHCCCCEEECCCCEEEEHHHHHHHHHHHHHHHHHHHCCCC
DIKVELTTDDSLVDIVQQGFDAGVRLSCIVEKDMISVAIGPPVKLCVAATPEYFARYGKP
CEEEEEECCHHHHHHHHHCCCCCCEEEEEEECCEEEEECCCCEEEEEECCHHHHHHCCCC
RHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNIILDDVDAELEAVLMGAGIGYLL
CCCHHHHCCCEEEEECCCCCCEEEEHHHHEEEEEECCEEEECCCHHHHHHHHHCCHHHHH
YEQIKEYLDTGRLECVLEDWSTERPGFQIYYPNRQYMSCGLRAFLDYVKTGQICQSQRHR
HHHHHHHHCCCCEEEEEECCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCHHHHHHCCC
PQ
CC
>Mature Secondary Structure
MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQRLKIRLFNRTTRSVSLT
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEECCCCCEEEEH
EAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARVAARIFLMSLLVGFTREYP
HHHHHHHHHHCCHHHHHHHHHHCCCCEEECCCCEEEEHHHHHHHHHHHHHHHHHHHCCCC
DIKVELTTDDSLVDIVQQGFDAGVRLSCIVEKDMISVAIGPPVKLCVAATPEYFARYGKP
CEEEEEECCHHHHHHHHHCCCCCCEEEEEEECCEEEEECCCCEEEEEECCHHHHHHCCCC
RHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNIILDDVDAELEAVLMGAGIGYLL
CCCHHHHCCCEEEEECCCCCCEEEEHHHHEEEEEECCEEEECCCHHHHHHHHHCCHHHHH
YEQIKEYLDTGRLECVLEDWSTERPGFQIYYPNRQYMSCGLRAFLDYVKTGQICQSQRHR
HHHHHHHHCCCCEEEEEECCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCHHHHHHCCC
PQ
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503