Click here to switch to the map view.

The map label for this gene is aat

Identifier: 157160407

GI number: 157160407

Start: 987076

End: 987780

Strand: Reverse

Name: aat

Synonym: EcHS_A0989

Alternate gene names: 157160407

Gene position: 987780-987076 (Counterclockwise)

Preceding gene: 157160408

Following gene: 157160406

Centisome position: 21.27

GC content: 53.9

Gene sequence:

>705_bases
ATGCGCCTGGTTCAGCTTTCTCGCCATTCAATAGCCTTCCCTTCCCCGGAAGGCGCATTACGTGAGCCTAACGGCCTGCT
GGCACTTGGGGGCGATCTTAGCCCTGCGCGCCTGTTAATGGCTTACCAGCGTGGTATTTTTCCGTGGTTTTCTCCAGGCG
ACCCCATCCTCTGGTGGTCGCCCGATCCCCGCGCGGTGCTATGGCCAGAATCACTGCATATCAGCCGTAGTATGAAGCGA
TTTCATAAACGCTCGCCCTATCGTGTCACGATGAATTACGCTTTTGGTCAGGTCATTGAAGGCTGTGCCAGCGATCGCGA
AGAAGGAACCTGGATCACGCGTGGCGTGGTCGAAGCCTACCATCGCCTTCACGAACTCGGTCATGCCCACTCCATTGAAG
TCTGGCGTGAAGATGAGCTTGTCGGCGGTATGTACGGCGTGGCCCAGGGAACACTATTTTGTGGCGAGTCCATGTTCAGC
CGGATGGAAAATGCGTCTAAAACGGCGCTTCTGGTATTCTGTGAGGAATTTATCGGTCATGGCGGTAAGCTTATCGACTG
CCAGGTCCTTAACGATCACACAGCATCGCTTGGTGCCTGCGAAATTCCCCGCCGGGATTACCTTAATTATCTCAATCAAA
TGCGCCTCGGACGATTGCCGAATAATTTCTGGGTACCACGGTGCTTGTTTTCACCACAAGAATGA

Upstream 100 bases:

>100_bases
CTCACGCAGAACTGCTTGCCAGACAGGGGCGTTATTACCAGTTCAAGCAGGGTTTGTAAGCTATTATTGAACGATCCGAC
TTGCGTGGAGTTTTGCGGTC

Downstream 100 bases:

>100_bases
ATGTTTTCGGCACATTTCTCCCCAGAGTGTTATAATTGCGGTCGCAGAGTTGGTTACGCTCATTACCCCGCTGCCGATAA
GGAATTTTTCGCGTCAGGTA

Product: leucyl/phenylalanyl-tRNA--protein transferase

Products: NA

Alternate protein names: L/F-transferase; Leucyltransferase; Phenyalanyltransferase

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQRGIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKR
FHKRSPYRVTMNYAFGQVIEGCASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGRLPNNFWVPRCLFSPQE

Sequences:

>Translated_234_residues
MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQRGIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKR
FHKRSPYRVTMNYAFGQVIEGCASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGRLPNNFWVPRCLFSPQE
>Mature_234_residues
MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQRGIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKR
FHKRSPYRVTMNYAFGQVIEGCASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGRLPNNFWVPRCLFSPQE

Specific function: Functions in the N-end rule pathway of protein degradation where it conjugates Leu, Phe and, less efficiently, Met from aminoacyl-tRNAs to the N-termini of proteins containing an N-terminal arginine or lysine

COG id: COG2360

COG function: function code O; Leu/Phe-tRNA-protein transferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the L/F-transferase family

Homologues:

Organism=Escherichia coli, GI1787111, Length=234, Percent_Identity=100, Blast_Score=486, Evalue=1e-139,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LFTR_ECO24 (A7ZJV2)

Other databases:

- EMBL:   CP000800
- RefSeq:   YP_001462081.1
- ProteinModelPortal:   A7ZJV2
- SMR:   A7ZJV2
- STRING:   A7ZJV2
- EnsemblBacteria:   EBESCT00000023100
- GeneID:   5586913
- GenomeReviews:   CP000800_GR
- KEGG:   ecw:EcE24377A_0958
- eggNOG:   COG2360
- GeneTree:   EBGT00050000011414
- HOGENOM:   HBG485363
- OMA:   GEPILWW
- ProtClustDB:   PRK00301
- BioCyc:   ECOL331111:ECE24377A_0958-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00688
- InterPro:   IPR016181
- InterPro:   IPR004616
- TIGRFAMs:   TIGR00667

Pfam domain/function: PF03588 Leu_Phe_trans; SSF55729 Acyl_CoA_acyltransferase

EC number: =2.3.2.6

Molecular weight: Translated: 26619; Mature: 26619

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQRGIFPWFSPGDPILWWS
CCEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCCEEEEC
PDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGCASDREEGTWITRGVVEAY
CCCCEEECCHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHCCCCCCCHHHHHHHHHH
HRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFSRMENASKTALLVFCEEFIGH
HHHHHHCCCCEEEEECCCCHHCCHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC
GGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGRLPNNFWVPRCLFSPQE
CCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQRGIFPWFSPGDPILWWS
CCEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCCEEEEC
PDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGCASDREEGTWITRGVVEAY
CCCCEEECCHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHCCCCCCCHHHHHHHHHH
HRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFSRMENASKTALLVFCEEFIGH
HHHHHHCCCCEEEEECCCCHHCCHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC
GGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGRLPNNFWVPRCLFSPQE
CCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA