Click here to switch to the map view.

The map label for this gene is hcr [H]

Identifier: 157160395

GI number: 157160395

Start: 971417

End: 972385

Strand: Reverse

Name: hcr [H]

Synonym: EcHS_A0976

Alternate gene names: 157160395

Gene position: 972385-971417 (Counterclockwise)

Preceding gene: 157160396

Following gene: 157160394

Centisome position: 20.94

GC content: 55.11

Gene sequence:

>969_bases
ATGACGATGCCAACGAATCAATGCCCGTGGCGGATGCAGGTTCATCACATTACGCAAGAAACGCCGGATGTGTGGACGAT
TTCCCTGATTTGCCACGATTACTACCCATATCGCGCCGGGCAATATGCACTGGTCAGCGTGCGAAACTCAGCGGAAACGC
TGCGTGCTTACACCATTTCCTCCACGCCAGGCGTGAGTGAATATATCACCCTGACCGTGCGGCGGATTGATGACGGTGTC
GGCTCCCAGTGGCTGACGCGCGATGTAAAACGCGGTGATTATCTCTGGCTTTCGGACGCGATGGGGGAATTTACCTGCGA
CGATAAAGCAGAAGATAAATTCCTGTTGCTGGCGGCAGGCTGCGGCGTCACGCCGATTATGTCGATGCGTCGCTGGCTGG
CGAAGAACTGTCCACAGGCCGATGTACAGGTGATCTACAACGTGCGTACGCCGCAGGATGTTATTTTCGCCGATGAGTGG
CGTAACTATCCGGTAACACTGGTGGCAGAAAATAACGTTACCGAAGGCTTTATCGCTGGTCGTCTCACTCGCGAACTGCT
GGCAGGTGTACCTGACTTAGCTTCACGTACCGTGATGACCTGCGGCCCGGCTCCGTATATGGATTGGGTAGAGCAGGAAG
TGAAAGCGCTCGGCGTGACGCGTTTCTTTAAAGAGAAATTCTTCACCCCAGTAGCAGAAGCGGCGACCAGCGGTCTGAAA
TTCACCAAACTGCAACCGGCACGAGAATTTTACGCCCCGGTTGGCACCACGCTACTGGAGGCGCTGGAAAGCAATAACGT
TCCGGTTGTCGCTGCCTGCCGTGCGGGTGTTTGCGGCTGCTGTAAGACAAAAGTGGTTTCCGGTGAATATACGGTGAGCA
GCACAATGACGCTGACCGACGCCGAAATCGCTGAAGGTTACGTACTGGCCTGCTCCTGCCATCCGCAGGGGGATTTGGTT
CTCGCATAA

Upstream 100 bases:

>100_bases
CAGACCTGCTGGCGGTGCTGAACGAGAAATTCGGCCTGCGTTCTATCACCACTGTTGAAGAAGACATGAAGCAACTGTTG
AGCGCGTAAGGAGTTTGTCG

Downstream 100 bases:

>100_bases
TCGCCTTATGCCCGATGATATTCCTTTCATCGGGCTATTTAACCGTTAGTGCCTCCTTTCTCTCCCATCCCTTCCCCCTC
CGTCAGATGAACTAAACTTG

Product: HCP oxidoreductase, NADH-dependent

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYALVSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGV
GSQWLTRDVKRGDYLWLSDAMGEFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNCPQADVQVIYNVRTPQDVIFADEW
RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALGVTRFFKEKFFTPVAEAATSGLK
FTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAGVCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLV
LA

Sequences:

>Translated_322_residues
MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYALVSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGV
GSQWLTRDVKRGDYLWLSDAMGEFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNCPQADVQVIYNVRTPQDVIFADEW
RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALGVTRFFKEKFFTPVAEAATSGLK
FTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAGVCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLV
LA
>Mature_321_residues
TMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYALVSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVG
SQWLTRDVKRGDYLWLSDAMGEFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNCPQADVQVIYNVRTPQDVIFADEWR
NYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALGVTRFFKEKFFTPVAEAATSGLKF
TKLQPAREFYAPVGTTLLEALESNNVPVVAACRAGVCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVL
A

Specific function: NADH oxidoreductase acting in concert with HCP [H]

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1787098, Length=322, Percent_Identity=99.3788819875776, Blast_Score=664, Evalue=0.0,
Organism=Escherichia coli, GI1787658, Length=338, Percent_Identity=28.4023668639053, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1788104, Length=289, Percent_Identity=28.0276816608997, Blast_Score=88, Evalue=9e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR000951
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 35659; Mature: 35528

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYALVSVRNSAETLRAYTIS
CCCCCCCCCCEEEEEECCCCCCCEEEEEEEEECCCCCCCCCEEEEEECCCCCEEEEEEEC
STPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMGEFTCDDKAEDKFLLLAAG
CCCCCCCEEEEEEEECCCCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCEEEEEEC
CGVTPIMSMRRWLAKNCPQADVQVIYNVRTPQDVIFADEWRNYPVTLVAENNVTEGFIAG
CCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEECCCCCCCEEEEECCCCCCHHHHH
RLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALGVTRFFKEKFFTPVAEAATSGLK
HHHHHHHCCCCCHHHCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCE
FTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAGVCGCCKTKVVSGEYTVSSTMTLTD
EEECCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCEEECCCEEEEEEEEEEC
AEIAEGYVLACSCHPQGDLVLA
HHHCCCEEEEEEECCCCCEEEC
>Mature Secondary Structure 
TMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYALVSVRNSAETLRAYTIS
CCCCCCCCCEEEEEECCCCCCCEEEEEEEEECCCCCCCCCEEEEEECCCCCEEEEEEEC
STPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMGEFTCDDKAEDKFLLLAAG
CCCCCCCEEEEEEEECCCCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCEEEEEEC
CGVTPIMSMRRWLAKNCPQADVQVIYNVRTPQDVIFADEWRNYPVTLVAENNVTEGFIAG
CCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEECCCCCCCEEEEECCCCCCHHHHH
RLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALGVTRFFKEKFFTPVAEAATSGLK
HHHHHHHCCCCCHHHCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCE
FTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAGVCGCCKTKVVSGEYTVSSTMTLTD
EEECCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCEEECCCEEEEEEEEEEC
AEIAEGYVLACSCHPQGDLVLA
HHHCCCEEEEEEECCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10651802 [H]