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The map label for this gene is amiD [H]

Identifier: 157160390

GI number: 157160390

Start: 965148

End: 965978

Strand: Direct

Name: amiD [H]

Synonym: EcHS_A0971

Alternate gene names: 157160390

Gene position: 965148-965978 (Clockwise)

Preceding gene: 157160389

Following gene: 157160399

Centisome position: 20.78

GC content: 56.2

Gene sequence:

>831_bases
ATGAGAAGAGTCTTCTGGCTGGTCGCTGCCGCTCTGTTATTGGCAGGGTGTGCAGGCGAAAAAGGCATTGTCGAGAAAGA
GGGATATCAGCTTGATACCCGACGCCAGGCGCAGGCGGCGTATCCGCGCATTAAAGTGCTGGTGATCCACTACACCGCAG
ATGATTTTGATAGCTCGCTGGCGACACTGACCGATAAGCAGGTCAGCTCGCATTATCTGGTCCCTGCGGTACCACCGCGA
TACAACGGTAAACCGCGCATCTGGCAACTGGTGCCGGAACAAGAACTGGCCTGGCATGCGGGGATTAGCGCCTGGCGCGG
GGCAACGCGCCTTAACGACACCTCTATTGGCATTGAGCTGGAAAACCGTGGCTGGCAAAAATCGGCCGGAGTGAAATATT
TTGCCCCGTTTGAACCGGCACAGATTCAGGCGCTTATTCCACTGGCGAAAGATATTATTGCCCGTTATCACATCAAGCCG
GAAAACGTAGTGGCACATGCGGATATCGCACCGCAGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGC
GCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAG
ATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGC
GTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGA
AGCATTGCTGGAGAAATACGGGCAGGATTAG

Upstream 100 bases:

>100_bases
CCGATGCCGTCGTCGGTATTGATATCGACTACGAAACGGTCGGGCAAAACGGCAGTATGCTGATGGTTAGCGTCAGCGGT
ACGGCGGTGAAAACGCGTCG

Downstream 100 bases:

>100_bases
CGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACC
TAACTCTTCCTGCGCACGCG

Product: N-acetylmuramoyl-L-alanine amidase amiD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRIKVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPR
YNGKPRIWQLVPEQELAWHAGISAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASLLELLARYGYDVKPDMTPREQRR
VIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD

Sequences:

>Translated_276_residues
MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRIKVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPR
YNGKPRIWQLVPEQELAWHAGISAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASLLELLARYGYDVKPDMTPREQRR
VIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
>Mature_276_residues
MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRIKVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPR
YNGKPRIWQLVPEQELAWHAGISAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASLLELLARYGYDVKPDMTPREQRR
VIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD

Specific function: Unknown

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787092, Length=276, Percent_Identity=99.6376811594203, Blast_Score=564, Evalue=1e-162,
Organism=Escherichia coli, GI1786300, Length=127, Percent_Identity=39.3700787401575, Blast_Score=79, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 31025; Mature: 31025

Theoretical pI: Translated: 7.78; Mature: 7.78

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRIKVLVIHYTADDFDSSL
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCEEEEEEEEEECCCCCCHH
ATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGISAWRGATRLNDTSIGIEL
HHHHHHHHHCCEECCCCCCCCCCCCCEEEECCCHHHHHHHCHHHHCCCCCCCCCEEEEEE
ENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKPENVVAHADIAPQRKDDPGPL
CCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCC
FPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASLLELLARYGYDVKPDMTPREQRR
CHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHH
VIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
EEEEEEHHCCCEEECCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRIKVLVIHYTADDFDSSL
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCEEEEEEEEEECCCCCCHH
ATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGISAWRGATRLNDTSIGIEL
HHHHHHHHHCCEECCCCCCCCCCCCCEEEECCCHHHHHHHCHHHHCCCCCCCCCEEEEEE
ENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKPENVVAHADIAPQRKDDPGPL
CCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCC
FPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASLLELLARYGYDVKPDMTPREQRR
CHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHH
VIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
EEEEEEHHCCCEEECCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]