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The map label for this gene is artI

Identifier: 157160386

GI number: 157160386

Start: 962492

End: 963223

Strand: Reverse

Name: artI

Synonym: EcHS_A0967

Alternate gene names: 157160386

Gene position: 963223-962492 (Counterclockwise)

Preceding gene: 157160387

Following gene: 157160385

Centisome position: 20.74

GC content: 50.27

Gene sequence:

>732_bases
ATGAAAAAAGTTCTGATTGCCGCGTTAATTGCAGGTTTTAGTCTTTCCGCCACAGCTGCCGAAACCATTCGTTTTGCTAC
CGAAGCCTCCTATCCTCCGTTTGAATCGATTGATGCAAACAACCAGATCGTTGGTTTTGACGTCGACCTGGCACAAGCGC
TGTGTAAAGAGATTGATGCAACCTGCACTTTCTCTAACCAGGCGTTTGACAGCCTGATCCCAAGCCTGAAATTCCGTCGC
GTAGAAGCCGTGATGGCGGGCATGGATATCACTCCGGAGCGTGAAAAGCAGGTGCTGTTTACCACCCCGTACTATGACAA
CTCTGCCCTGTTTGTGGGTCAGCAAGGCAAATACACCAGTGTTGATCAGCTGAAAGGCAAAAAAGTCGGCGTACAGAACG
GGACGACACACCAGAAATTCATTATGGATAAGCACCCGGAAATCACTACCGTTCCGTATGACAGCTACCAGAACGCAAAA
CTGGATCTGCAAAACGGGCGTATCGACGGCGTCTTCGGTGACACCGCAGTGGTCACTGAGTGGCTGAAAGATAACCCGAA
ACTGGCGGCGGTGGGCGACAAAGTGACCGATAAAGATTACTTCGGCACTGGCCTCGGCATCGCGGTACGTCAGGGCAACA
CTGAGCTGCAGCAGAAACTCAACACTGCGCTGGAAAAAGTGAAGAAAGATGGCACTTACGAAACCATCTACAACAAATGG
TTCCAGAAGTAA

Upstream 100 bases:

>100_bases
ATGGTCATATCGTAGAACAAGGCGACGCGAGCTGCTTTACCGAGCCGCAAACCGAAGCATTTAAAAACTATCTCTCTCAC
TAAGATTCGGGATAACAACA

Downstream 100 bases:

>100_bases
TTCCTGATGAATGAATTTTTTCCTTTAGCAAGCGCCGCCGGGATGACCGTCGGCCTTGCCGTTTGTGCATTGATTGTCGG
GCTGGCGCTGGCGATGTTCT

Product: arginine ABC transporter, periplasmic arginine-binding protein ArtI

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIVGFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRR
VEAVMAGMDITPEREKQVLFTTPYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQKLNTALEKVKKDGTYETIYNKW
FQK

Sequences:

>Translated_243_residues
MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIVGFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRR
VEAVMAGMDITPEREKQVLFTTPYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQKLNTALEKVKKDGTYETIYNKW
FQK
>Mature_243_residues
MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIVGFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRR
VEAVMAGMDITPEREKQVLFTTPYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQKLNTALEKVKKDGTYETIYNKW
FQK

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family

Homologues:

Organism=Escherichia coli, GI1787088, Length=243, Percent_Identity=100, Blast_Score=502, Evalue=1e-144,
Organism=Escherichia coli, GI1787085, Length=241, Percent_Identity=64.7302904564315, Blast_Score=343, Evalue=6e-96,
Organism=Escherichia coli, GI1788649, Length=253, Percent_Identity=33.596837944664, Blast_Score=158, Evalue=3e-40,
Organism=Escherichia coli, GI1788648, Length=229, Percent_Identity=38.4279475982533, Blast_Score=158, Evalue=4e-40,
Organism=Escherichia coli, GI1788228, Length=252, Percent_Identity=30.1587301587302, Blast_Score=124, Evalue=5e-30,
Organism=Escherichia coli, GI1787031, Length=244, Percent_Identity=33.6065573770492, Blast_Score=122, Evalue=2e-29,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): ARTI_ECOLI (P30859)

Other databases:

- EMBL:   X86160
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   G64824
- RefSeq:   AP_001494.1
- RefSeq:   NP_415384.1
- ProteinModelPortal:   P30859
- SMR:   P30859
- STRING:   P30859
- SWISS-2DPAGE:   P30859
- EnsemblBacteria:   EBESCT00000001514
- EnsemblBacteria:   EBESCT00000017016
- GeneID:   948988
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW0847
- KEGG:   eco:b0863
- EchoBASE:   EB1582
- EcoGene:   EG11625
- eggNOG:   COG0834
- GeneTree:   EBGT00050000009492
- HOGENOM:   HBG698334
- OMA:   QDGTYET
- ProtClustDB:   PRK15007
- BioCyc:   EcoCyc:ARTI-MONOMER
- Genevestigator:   P30859
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313
- SMART:   SM00062
- TIGRFAMs:   TIGR01096

Pfam domain/function: PF00497 SBP_bac_3

EC number: NA

Molecular weight: Translated: 26930; Mature: 26930

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIVGFDVDLAQALCKEIDA
CHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECHHHHHHHHHHCCC
TCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTTPYYDNSALFVGQQGKYTS
EEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCEEEEECCCCCCC
VDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAKLDLQNGRIDGVFGDTAVVTE
HHHHCCCEECCCCCCCHHHHHHCCCCCEEEECCCCCCCCEEEECCCEECEEECCHHHHHH
WLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQKLNTALEKVKKDGTYETIYNKW
HHHCCCCEEEECCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHH
FQK
HCC
>Mature Secondary Structure
MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIVGFDVDLAQALCKEIDA
CHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECHHHHHHHHHHCCC
TCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTTPYYDNSALFVGQQGKYTS
EEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCEEEEECCCCCCC
VDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAKLDLQNGRIDGVFGDTAVVTE
HHHHCCCEECCCCCCCHHHHHHCCCCCEEEECCCCCCCCEEEECCCEECEEECCHHHHHH
WLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQKLNTALEKVKKDGTYETIYNKW
HHHCCCCEEEECCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHH
FQK
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841