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The map label for this gene is artJ [H]

Identifier: 157160383

GI number: 157160383

Start: 960144

End: 960875

Strand: Reverse

Name: artJ [H]

Synonym: EcHS_A0964

Alternate gene names: 157160383

Gene position: 960875-960144 (Counterclockwise)

Preceding gene: 157160384

Following gene: 157160371

Centisome position: 20.69

GC content: 45.77

Gene sequence:

>732_bases
ATGAAAAAGTTAGTTCTTGCCGCTTTACTTGCTTCCTTTACTTTCGGTGCTTCTGCCGCAGAGAAAATCAATTTTGGCGT
TTCAGCCACCTATCCCCCCTTTGAATCTATAGGTGCTAATAATGAGATTGTCGGCTTTGATATCGATCTGGCAAAAGCCT
TGTGCAAACAAATGCAGGCAGAATGTACTTTTACTAATCACGCGTTCGACAGCCTGATCCCGTCACTGAAATTCAGAAAA
TATGACGCCGTAATCTCCGGTATGGATATCACGCCCGAGCGTAGCAAGCAGGTATCGTTTACCACGCCCTACTATGAAAA
CTCAGCCGTCGTGATTGCCAAAAAAGATACCTACAAAACGTTTGCCGATCTGAAAGGCAAACGTATTGGGATGGAAAACG
GCACCACGCACCAGAAATATATTCAGGATCAGCACCCCGAAGTGAAAACTGTCTCCTATGACAGTTATCAGAATGCCTTT
ATCGATCTGAAAAATGGTCGTATTGATGGGGTATTTGGTGACACAGCGGTGGTAAACGAATGGCTGAAAACCAATCCGCA
ACTGGGCGTTGCTACTGAAAAAGTGACCGATCCGCAATACTTCGGCACCGGTCTGGGCATCGCTGTACGTCCGGATAACA
AAGCTCTGCTGGAAAAACTGAATAACGCGCTGGCAGCAATTAAAGCTGACGGTACATATCAGAAAATCAATGACCAGTGG
TTCCCGCAGTAA

Upstream 100 bases:

>100_bases
TTTAATCATATTTATTGCATATAAATTCACTTGATGGCATTGTTATCCCATGCCGCAGACACGGCCAAAAATCATAAGAT
TGACAGACGGGAGTTCCATC

Downstream 100 bases:

>100_bases
GATTCAGAAAGCCGCAACCGCGGCTTGAGACTGATGACAAAGGTAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACA
TGGCCCATGCAATGTATTGA

Product: arginine ABC transporter, periplasmic arginine-binding protein ArtJ

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIVGFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRK
YDAVISGMDITPERSKQVSFTTPYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLEKLNNALAAIKADGTYQKINDQW
FPQ

Sequences:

>Translated_243_residues
MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIVGFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRK
YDAVISGMDITPERSKQVSFTTPYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLEKLNNALAAIKADGTYQKINDQW
FPQ
>Mature_243_residues
MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIVGFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRK
YDAVISGMDITPERSKQVSFTTPYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLEKLNNALAAIKADGTYQKINDQW
FPQ

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport. Binds L-arginine with high affinity [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787085, Length=243, Percent_Identity=99.5884773662551, Blast_Score=503, Evalue=1e-144,
Organism=Escherichia coli, GI1787088, Length=241, Percent_Identity=64.7302904564315, Blast_Score=343, Evalue=7e-96,
Organism=Escherichia coli, GI1788648, Length=229, Percent_Identity=40.174672489083, Blast_Score=168, Evalue=3e-43,
Organism=Escherichia coli, GI1788649, Length=253, Percent_Identity=35.9683794466403, Blast_Score=160, Evalue=7e-41,
Organism=Escherichia coli, GI1788228, Length=220, Percent_Identity=32.7272727272727, Blast_Score=130, Evalue=7e-32,
Organism=Escherichia coli, GI1787031, Length=244, Percent_Identity=29.5081967213115, Blast_Score=108, Evalue=2e-25,

Paralogues:

None

Copy number: 1180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 26857; Mature: 26857

Theoretical pI: Translated: 7.49; Mature: 7.49

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIVGFDIDLAKALCKQMQA
CHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCHHHCCCCCCEEEECHHHHHHHHHHHHH
ECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTTPYYENSAVVIAKKDTYKT
CCEECHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCEEEEECCCCCCCEEEEEECCCHHH
FADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAFIDLKNGRIDGVFGDTAVVNE
HHHCCCCEECCCCCCCHHHHHHCCCCCEEEEECCCCCCEEEEECCCEECCEECCHHHHHH
WLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLEKLNNALAAIKADGTYQKINDQW
HHHCCCCCCCEECCCCCCHHHCCCCEEEECCCCHHHHHHHHHHHEEEECCCCCEECCCCC
FPQ
CCC
>Mature Secondary Structure
MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIVGFDIDLAKALCKQMQA
CHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCHHHCCCCCCEEEECHHHHHHHHHHHHH
ECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTTPYYENSAVVIAKKDTYKT
CCEECHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCEEEEECCCCCCCEEEEEECCCHHH
FADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAFIDLKNGRIDGVFGDTAVVNE
HHHCCCCEECCCCCCCHHHHHHCCCCCEEEEECCCCCCEEEEECCCEECCEECCHHHHHH
WLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLEKLNNALAAIKADGTYQKINDQW
HHHCCCCCCCEECCCCCCHHHCCCCEEEECCCCHHHHHHHHHHHEEEECCCCCEECCCCC
FPQ
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]