Click here to switch to the map view.

The map label for this gene is rimO [H]

Identifier: 157160316

GI number: 157160316

Start: 905546

End: 906871

Strand: Reverse

Name: rimO [H]

Synonym: EcHS_A0895

Alternate gene names: 157160316

Gene position: 906871-905546 (Counterclockwise)

Preceding gene: 157160318

Following gene: 157160315

Centisome position: 19.53

GC content: 52.79

Gene sequence:

>1326_bases
ATGAGCAAAGTAACTCCCCAGCCGAAAATCGGCTTTGTTTCCCTTGGCTGTCCGAAAAACCTTGTCGATTCAGAGCGTAT
TCTCACCGAACTCCGCACTGAAGGTTATGACGTGGTACCGAGCTATGACGATGCGGACATGGTGATCGTCAACACCTGCG
GCTTTATTGACAGCGCGGTACAAGAATCACTGGAAGCCATTGGTGAAGCGTTGAATGAAAACGGCAAGGTAATTGTGACC
GGTTGTCTGGGGGCAAAAGAAGATCAGATCCGCGAAGTCCACCCGAAAGTGCTGGAAATCACCGGGCCTCATAGCTATGA
GCAGGTTCTGGAGCACGTTCATCACTACGTGCCAAAACCGAAACACAACCCATTCCTGAGCCTGGTGCCAGAACAAGGTG
TGAAACTGACGCCGCGTCATTATGCCTATCTGAAAATTTCGGAAGGCTGTAATCACCGCTGCACCTTCTGCATTATTCCG
TCTATGCGCGGCGACCTGGTGAGCCGTCCGATTGGCGAAGTGTTAAGTGAAGCGAAACGTCTGGTAGATGCGGGCGTTAA
AGAGATTCTGGTGATCTCGCAGGATACTTCCGCCTATGGCGTTGATGTTAAACATCGTACTGGCTTCCACAACGGCGAGC
CGGTAAAAACCAGCATGGTCAGCCTGTGCGAACAGTTATCGAAACTGGGGATCTGGACACGTCTGCACTACGTTTACCCT
TATCCGCATGTGGACGACGTCATCCCACTGATGGCAGAAGGCAAAATCCTGCCGTATCTGGACATTCCGTTGCAGCACGC
CAGCCCGCGCATTCTCAAACTGATGAAGCGTCCGGGTTCTGTAGATCGCCAACTGGCGCGCATCAAACAGTGGCGCGAAA
TCTGCCCGGAACTGACCCTACGCTCAACCTTTATTGTCGGCTTCCCTGGCGAGACAGAAGAAGATTTCCAGATGCTACTC
GACTTCCTGAAAGAAGCGCGTCTGGATCGCGTTGGCTGCTTTAAATACAGCCCGGTTGAAGGTGCAGACGCCAATGCCCT
GCCTGACCAGGTTCCGGAAGAAGTTAAAGAAGAACGCTGGAACCGTTTCATGCAGCTGCAGCAGCAGATTTCCGCCGAGC
GCCTGCAAGAGAAAGTGGGCCGTGAAATTCTGGTGATTATCGACGAAGTGGACGAAGAAGGCGCGATTGGTCGCAGCATG
GCAGATGCACCGGAAATCGACGGCGCGGTTTATCTCAATGGTGAAACCAACGTTAAGCCGGGTGATATCCTGCGTGTGAA
AGTCGAGCACGCCGATGAGTACGATTTGTGGGGTAGCCGGGTTTAA

Upstream 100 bases:

>100_bases
CTTCGCCTCCGCCAGCGAATGAGATTGCGATACAAATTTTATCCAGAACTCTGTAGAATCCCTGCCCTGACCTTTCCTGT
AACTGAATATTTTTTAAGCT

Downstream 100 bases:

>100_bases
TTAATCTAAAGGCAGATCTAACAAGGATATTGTTCTTCGATCTGCCTCTACTACCCTTCCCGATGTCCTACCCTTACGCA
CAAAATCCCCTGACGCATAA

Product: MiaB-like tRNA modifying enzyme YliG

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO [H]

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDDADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVT
GCLGAKEDQIREVHPKVLEITGPHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTSMVSLCEQLSKLGIWTRLHYVYP
YPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMKRPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLL
DFLKEARLDRVGCFKYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDEEGAIGRSM
ADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV

Sequences:

>Translated_441_residues
MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDDADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVT
GCLGAKEDQIREVHPKVLEITGPHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTSMVSLCEQLSKLGIWTRLHYVYP
YPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMKRPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLL
DFLKEARLDRVGCFKYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDEEGAIGRSM
ADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV
>Mature_440_residues
SKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDDADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTG
CLGAKEDQIREVHPKVLEITGPHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIPS
MRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTSMVSLCEQLSKLGIWTRLHYVYPY
PHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMKRPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLD
FLKEARLDRVGCFKYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDEEGAIGRSMA
DAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12 [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI93277076, Length=409, Percent_Identity=26.8948655256724, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI28872784, Length=474, Percent_Identity=25.9493670886076, Blast_Score=142, Evalue=8e-34,
Organism=Homo sapiens, GI28872782, Length=474, Percent_Identity=25.9493670886076, Blast_Score=142, Evalue=8e-34,
Organism=Escherichia coli, GI1787057, Length=441, Percent_Identity=100, Blast_Score=905, Evalue=0.0,
Organism=Escherichia coli, GI1786882, Length=385, Percent_Identity=28.3116883116883, Blast_Score=150, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17553146, Length=465, Percent_Identity=26.0215053763441, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI71996771, Length=212, Percent_Identity=29.2452830188679, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21356207, Length=479, Percent_Identity=25.678496868476, Blast_Score=149, Evalue=5e-36,
Organism=Drosophila melanogaster, GI19922432, Length=411, Percent_Identity=25.7907542579075, Blast_Score=112, Evalue=8e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 49582; Mature: 49451

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDDADMVIVNTCGFIDSAV
CCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCHHHHHH
QESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITGPHSYEQVLEHVHHYVPKP
HHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCC
KHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIPSMRGDLVSRPIGEVLSEAKR
CCCCCEEECCCCCCEECCCEEEEEEECCCCCCCEEEEEECCCCCHHHHCCHHHHHHHHHH
LVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTSMVSLCEQLSKLGIWTRLHYVYP
HHHCCCCEEEEEECCCCCEEEEEHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHEEEEEEC
YPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMKRPGSVDRQLARIKQWREICPELTL
CCCHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEE
RSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCFKYSPVEGADANALPDQVPEEVKEERW
CCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCHHHHHHHHHHHH
NRFMQLQQQISAERLQEKVGREILVIIDEVDEEGAIGRSMADAPEIDGAVYLNGETNVKP
HHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCCCC
GDILRVKVEHADEYDLWGSRV
CCEEEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
SKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDDADMVIVNTCGFIDSAV
CCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCHHHHHH
QESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITGPHSYEQVLEHVHHYVPKP
HHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCC
KHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIPSMRGDLVSRPIGEVLSEAKR
CCCCCEEECCCCCCEECCCEEEEEEECCCCCCCEEEEEECCCCCHHHHCCHHHHHHHHHH
LVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTSMVSLCEQLSKLGIWTRLHYVYP
HHHCCCCEEEEEECCCCCEEEEEHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHEEEEEEC
YPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMKRPGSVDRQLARIKQWREICPELTL
CCCHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEE
RSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCFKYSPVEGADANALPDQVPEEVKEERW
CCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCHHHHHHHHHHHH
NRFMQLQQQISAERLQEKVGREILVIIDEVDEEGAIGRSMADAPEIDGAVYLNGETNVKP
HHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCCCC
GDILRVKVEHADEYDLWGSRV
CCEEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA