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The map label for this gene is iaaA [H]

Identifier: 157160307

GI number: 157160307

Start: 894450

End: 895415

Strand: Direct

Name: iaaA [H]

Synonym: EcHS_A0886

Alternate gene names: 157160307

Gene position: 894450-895415 (Clockwise)

Preceding gene: 157160304

Following gene: 157160308

Centisome position: 19.26

GC content: 56.31

Gene sequence:

>966_bases
ATGGGCAAAGCAGTCATTGCAATTCATGGTGGCGCAGGTGCAATTAGCCGCGCGCAGATGAGTCTGCAACAGGAATTACG
CTACATTGAGGCGTTGTCTGCCATTGTTGAAACCGGGCAGAAAATGCTGGAAGCGGGCGAAAGTGCGCTGGATGTGGTGA
CGGAAGCGGTGCGTCTGCTGGAAGAGTGTCCACTGTTTAACGCCGGAATTGGCGCTGTCTTTACGCGTGATGAAACCCAT
GAACTGGACGCCAGTGTGATGGATGGTAACACCCTGAAAGCCGGTGCGGTGGCGGGCGTTAGTCATCTGCGTAATCCGGT
TCTTGCCGCCCGGCTGGTGATGGAGCAAAGCCCGCATGTGATGATGATTGGCGAAGGGGCAGAAAATTTTGCGTTTGCTC
ATGGCATGGAGCGCGTCTCACCGGAGATTTTCTCCACGCCTTTGCGTTATGAACAACTACTGGCAGCGCGCGAGGAAGGG
GCAACAGTCCTCGACCATAGCGGTGCGCCACTGGATGAAAAACAGAAAATGGGCACCGTGGGGGCCGTGGCGTTGGATTT
AGACGGCAATCTGGCGGCAGCCACGTCCACAGGCGGAATGACCAATAAATTACCCGGACGAGTTGGCGATAGTCCCTTAG
TGGGTGCCGGATGCTACGCCAATAACGCCAGTGTGGCGGTTTCTTGTACCGGCACGGGCGAAGTCTTCATCCGCGCGCTG
ACGGCATATGACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTAATGGAAAA
ACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAG
GAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAAGGGGACACCGTTGCCACA
CAGTGA

Upstream 100 bases:

>100_bases
GCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAATTCTGATATTTATAGCAAAAGTGGCGAGCC
ACCCTTAATGGACGAATACT

Downstream 100 bases:

>100_bases
TGAACTTGATGCCGGTGATGTGCTGGCGGTTGAAAATCTGAAAATTGCCTTTATGCAGGACCAGCAGAAAATAGCTGCGG
TCCGCAATCTCTCTTTTAGC

Product: L-asparaginase

Products: NA

Alternate protein names: Beta-aspartyl-peptidase; EcAIII; Isoaspartyl dipeptidase [H]

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLEAGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETH
ELDASVMDGNTLKAGAVAGVSHLRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGCYANNASVAVSCTGTGEVFIRAL
TAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGGLIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVAT
Q

Sequences:

>Translated_321_residues
MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLEAGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETH
ELDASVMDGNTLKAGAVAGVSHLRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGCYANNASVAVSCTGTGEVFIRAL
TAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGGLIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVAT
Q
>Mature_320_residues
GKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLEAGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHE
LDASVMDGNTLKAGAVAGVSHLRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEGA
TVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGCYANNASVAVSCTGTGEVFIRALT
AYDIAALMDYGGLSLAEACERVVMEKLPALGGSGGLIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ

Specific function: May be involved in glutathione, and possibly other peptide, transport, although these results could also be due to polar effects of disruption [H]

COG id: COG1446

COG function: function code E; Asparaginase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Ntn-hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI145275202, Length=305, Percent_Identity=38.0327868852459, Blast_Score=172, Evalue=5e-43,
Organism=Homo sapiens, GI145275200, Length=305, Percent_Identity=38.0327868852459, Blast_Score=172, Evalue=5e-43,
Organism=Homo sapiens, GI98991769, Length=269, Percent_Identity=33.457249070632, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI285002251, Length=256, Percent_Identity=28.90625, Blast_Score=88, Evalue=1e-17,
Organism=Escherichia coli, GI1787050, Length=321, Percent_Identity=98.4423676012461, Blast_Score=638, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17569239, Length=252, Percent_Identity=27.7777777777778, Blast_Score=84, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI32564649, Length=311, Percent_Identity=26.6881028938907, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI18921183, Length=322, Percent_Identity=36.6459627329193, Blast_Score=171, Evalue=5e-43,
Organism=Drosophila melanogaster, GI21357893, Length=268, Percent_Identity=30.9701492537313, Blast_Score=118, Evalue=5e-27,
Organism=Drosophila melanogaster, GI24652135, Length=236, Percent_Identity=33.4745762711864, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI221330102, Length=236, Percent_Identity=33.4745762711864, Blast_Score=104, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000246 [H]

Pfam domain/function: PF01112 Asparaginase_2 [H]

EC number: =3.4.19.5 [H]

Molecular weight: Translated: 33400; Mature: 33269

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLEAGESALDVVTEAVRLL
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
EECPLFNAGIGAVFTRDETHELDASVMDGNTLKAGAVAGVSHLRNPVLAARLVMEQSPHV
HHCCCCCCCCCEEEECCCCHHCCCEEECCCCCCHHHHHHHHHHHCHHHHHHHHHCCCCCE
MMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEGATVLDHSGAPLDEKQKMGTV
EEECCCCCHHHHHHHHHHHCHHHHCCCHHHHHHHHHHHCCCEEEECCCCCCCHHHHCCCE
GAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGCYANNASVAVSCTGTGEVFIRAL
EEEEEECCCCEEEEECCCCCCCCCCCCCCCCCCEECCEECCCCEEEEEECCCHHHHHHHH
TAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGGLIAIDHEGNVALPFNTEGMYRAWG
HHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEEECCCCCEEECC
YAGDTPTTGIYREKGDTVATQ
CCCCCCCCCEEECCCCCCCCC
>Mature Secondary Structure 
GKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLEAGESALDVVTEAVRLL
CCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
EECPLFNAGIGAVFTRDETHELDASVMDGNTLKAGAVAGVSHLRNPVLAARLVMEQSPHV
HHCCCCCCCCCEEEECCCCHHCCCEEECCCCCCHHHHHHHHHHHCHHHHHHHHHCCCCCE
MMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEGATVLDHSGAPLDEKQKMGTV
EEECCCCCHHHHHHHHHHHCHHHHCCCHHHHHHHHHHHCCCEEEECCCCCCCHHHHCCCE
GAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGCYANNASVAVSCTGTGEVFIRAL
EEEEEECCCCEEEEECCCCCCCCCCCCCCCCCCEECCEECCCCEEEEEECCCHHHHHHHH
TAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGGLIAIDHEGNVALPFNTEGMYRAWG
HHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEEECCCCCEEECC
YAGDTPTTGIYREKGDTVATQ
CCCCCCCCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 3045084; 7984428 [H]