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The map label for this gene is rlmF [H]

Identifier: 157160283

GI number: 157160283

Start: 868855

End: 869862

Strand: Direct

Name: rlmF [H]

Synonym: EcHS_A0862

Alternate gene names: 157160283

Gene position: 868855-869862 (Clockwise)

Preceding gene: 157160277

Following gene: 157160292

Centisome position: 18.71

GC content: 53.67

Gene sequence:

>1008_bases
TTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGC
TATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTACGATCTCGCCACGCTTTGTCAGGTCA
ATCCTGAACTCAGGCAATTCCTCACGCTTACACCCGCCGGGGAGCAAAGCGTAGACTTTGCCAATCCGCTGGCGGTGAAG
GCGCTCAATAAGGCGTTGCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGACGGTTTTCTCTGCCCACCAGT
ACCGGGCCGGGCGGATTATATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGCGAATGCCAGCA
TTCTGGATATCGGCGTTGGTGCGAACTGTATTTATCCGCTGATTGGCGTACATGAATATGGCTGGCGTTTTACCGGTAGT
GAAACCAGCAGCCAGGCGTTAAGCAGTGCGCAGGCGATTATCAGTTCTAATCCGGGGCTTAACCGCGCCATTCGTCTGCG
TCGGCAAAAAGAGAGTGGGGCGATTTTTAACGGCATCATCCATAAAAACGAGCAATACGACGCGACCTTGTGTAACCCGC
CATTCCACGATTCCGCCGCTGCGGCACGGGCAGGTAGTGAGCGTAAACGCCGTAACCTGGGGCTGAACAAAGACGATGCA
CTGAACTTTGGCGGCCAGCAACAGGAGTTGTGGTGTGAAGGCGGTGAAGTCACCTTTATCAAAAAGATGATTGAAGAGAG
CAAAGGCTTCGCGAAGCAGGTGATGTGGTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCC
TGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACC
TTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAA

Upstream 100 bases:

>100_bases
TTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAA
ACCATAGCCATGTTAAGGTT

Downstream 100 bases:

>100_bases
AGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGC
GCGAACGCCCGCCAGGTCGA

Product: putative SAM-dependent methyltransferase

Products: NA

Alternate protein names: 23S rRNA mA1618 methyltransferase; rRNA adenine N-6-methyltransferase [H]

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MGNDKRSQIFTLSRYAGILRRCLKENAMSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSVDFANPLAVK
ALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIPANASILDIGVGANCIYPLIGVHEYGWRFTGS
ETSSQALSSAQAIISSNPGLNRAIRLRRQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDA
LNFGGQQQELWCEGGEVTFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVVKKEMAQGQKQSRFIAWT
FMNDEQRRRFVNRQR

Sequences:

>Translated_335_residues
MGNDKRSQIFTLSRYAGILRRCLKENAMSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSVDFANPLAVK
ALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIPANASILDIGVGANCIYPLIGVHEYGWRFTGS
ETSSQALSSAQAIISSNPGLNRAIRLRRQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDA
LNFGGQQQELWCEGGEVTFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVVKKEMAQGQKQSRFIAWT
FMNDEQRRRFVNRQR
>Mature_334_residues
GNDKRSQIFTLSRYAGILRRCLKENAMSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSVDFANPLAVKA
LNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIPANASILDIGVGANCIYPLIGVHEYGWRFTGSE
TSSQALSSAQAIISSNPGLNRAIRLRRQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDAL
NFGGQQQELWCEGGEVTFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVVKKEMAQGQKQSRFIAWTF
MNDEQRRRFVNRQR

Specific function: Specifically methylates the adenine in position 1618 of 23S rRNA [H]

COG id: COG3129

COG function: function code R; Predicted SAM-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. METT10D/rlmF family [H]

Homologues:

Organism=Homo sapiens, GI122114654, Length=293, Percent_Identity=30.7167235494881, Blast_Score=148, Evalue=8e-36,
Organism=Escherichia coli, GI87081786, Length=308, Percent_Identity=100, Blast_Score=644, Evalue=0.0,
Organism=Caenorhabditis elegans, GI32564931, Length=308, Percent_Identity=26.2987012987013, Blast_Score=120, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI32564933, Length=308, Percent_Identity=24.3506493506494, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI281363415, Length=291, Percent_Identity=29.553264604811, Blast_Score=124, Evalue=9e-29,
Organism=Drosophila melanogaster, GI19922302, Length=291, Percent_Identity=29.553264604811, Blast_Score=124, Evalue=9e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016909
- InterPro:   IPR010286 [H]

Pfam domain/function: PF05971 Methyltransf_10 [H]

EC number: =2.1.1.181 [H]

Molecular weight: Translated: 37350; Mature: 37219

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNDKRSQIFTLSRYAGILRRCLKENAMSAQKPGLHPRNRHHSRYDLATLCQVNPELRQF
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHH
LTLTPAGEQSVDFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADL
EECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHH
LAEASGTIPANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISSNPGL
HHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHCCCCH
NRAIRLRRQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDA
HHHHHHHHHHHCCCEEHHHHCCCCCCCCEECCCCCCCCHHHHHCCHHHHHHHCCCCCCCC
LNFGGQQQELWCEGGEVTFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAV
CCCCCCCHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
KVVKKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR
HHHHHHHHCCHHHCEEEEEEECCHHHHHHHHCCCC
>Mature Secondary Structure 
GNDKRSQIFTLSRYAGILRRCLKENAMSAQKPGLHPRNRHHSRYDLATLCQVNPELRQF
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHH
LTLTPAGEQSVDFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADL
EECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHH
LAEASGTIPANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISSNPGL
HHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHCCCCH
NRAIRLRRQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDA
HHHHHHHHHHHCCCEEHHHHCCCCCCCCEECCCCCCCCHHHHHCCHHHHHHHCCCCCCCC
LNFGGQQQELWCEGGEVTFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAV
CCCCCCCHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
KVVKKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR
HHHHHHHHCCHHHCEEEEEEECCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA