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The map label for this gene is zitB

Identifier: 157160227

GI number: 157160227

Start: 810483

End: 811424

Strand: Reverse

Name: zitB

Synonym: EcHS_A0806

Alternate gene names: 157160227

Gene position: 811424-810483 (Counterclockwise)

Preceding gene: 157160228

Following gene: 157160214

Centisome position: 17.47

GC content: 53.08

Gene sequence:

>942_bases
ATGGCGCACTCACACTCACACACGTCCTCACACCTGCCAGAAGATAATAATGCTCGTCGCTTGTTGTATGCTTTCGGCGT
GACTGCCGGGTTTATGCTGGTAGAAGTCGTTGGTGGTTTTCTTTCTGGTTCTCTGGCATTGCTGGCCGATGCGGGTCATA
TGTTGACCGATACTGCCGCCCTGCTTTTCGCCCTACTCGCCGTGCAATTTTCACGTCGTCCTCCCACCATTCGCCACACT
TTCGGTTGGCTAAGACTGACCACGCTCGCGGCGTTTGTGAATGCCATAGCCCTCGTGGTAATTACCATTTTGATTGTCTG
GGAGGCGATAGAACGCTTCCGCACGCCGCGTCCGGTCGAGGGCGGCATGATGATGGCAATTGCCGTGGCTGGGTTGCTGG
CAAATATACTTTCTTTCTGGTTACTTCATCACGGCAGCGAAGAGAAAAACCTCAACGTACGAGCAGCGGCACTGCATGTG
CTCGGGGACCTGCTGGGTTCGGTTGGGGCTATTATTGCCGCGTTGATTATTATCTGGACTGGCTGGACACCTGCTGACCC
CATTCTCTCGATACTGGTGTCGCTTCTGGTTCTGCGTAGCGCGTGGCGACTGTTGAAAGATAGCGTGAATGAATTACTTG
AAGGTGCACCGGTATCGCTGGATATCGCTGAACTGAAGCGTCGTATGTGCCGGGAAATCCCGGAAGTCCGCAATGTGCAC
CATGTACATGTATGGATGGTAGGCGAGAAGCCGGTGATGACGTTGCATGTGCAGGTGATCCCGCCACACGATCACGATGC
CTTGTTGGATCAGATCCAACACTACCTGATGGATCACTATCAGATTGAGCACGCCACCATTCAGATGGAATATCAACCTT
GTCATGGGCCAGACTGCCACCTGAATGAAGGGGTGTCTGGCCATTCACATCATCACCATTAA

Upstream 100 bases:

>100_bases
CCAATAATGGGAGAGCGGAGTCTCTCCCTTTTAATTTTTCACTTCTAAAAGCAGTATGATGTCATAAAGTTAAGTGAGAA
TAATTAAGGGATGACTCGTC

Downstream 100 bases:

>100_bases
TGGGACAGCGCGCGTGAGCCTCTTTCACGTGCGCTGTTGATCCACATCCGGCTGCCGTTGAGCGCAATAAAGGTCAGGAT
GATGTACTCCAGAGACATGG

Product: zinc transporter ZitB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGSLALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHT
FGWLRLTTLAAFVNAIALVVITILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPVSLDIAELKRRMCREIPEVRNVH
HVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYLMDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH

Sequences:

>Translated_313_residues
MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGSLALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHT
FGWLRLTTLAAFVNAIALVVITILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPVSLDIAELKRRMCREIPEVRNVH
HVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYLMDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH
>Mature_312_residues
AHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGSLALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTF
GWLRLTTLAAFVNAIALVVITILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHVL
GDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPVSLDIAELKRRMCREIPEVRNVHH
VHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYLMDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH

Specific function: Involved in zinc efflux across the cytoplasmic membrane, thus reducing zinc accumulation in the cytoplasm and rendering bacteria more resistant to zinc. It may contribute to zinc homeostasis at low concentrations of zinc, whereas zntA is required for grow

COG id: COG1230

COG function: function code P; Co/Zn/Cd efflux system component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily

Homologues:

Organism=Homo sapiens, GI52352805, Length=320, Percent_Identity=32.5, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI289803013, Length=320, Percent_Identity=32.8125, Blast_Score=149, Evalue=2e-36,
Organism=Homo sapiens, GI289803009, Length=320, Percent_Identity=32.8125, Blast_Score=149, Evalue=2e-36,
Organism=Homo sapiens, GI289803007, Length=320, Percent_Identity=32.8125, Blast_Score=149, Evalue=2e-36,
Organism=Homo sapiens, GI289803003, Length=320, Percent_Identity=32.8125, Blast_Score=149, Evalue=2e-36,
Organism=Homo sapiens, GI64762489, Length=320, Percent_Identity=32.8125, Blast_Score=149, Evalue=3e-36,
Organism=Homo sapiens, GI21361112, Length=309, Percent_Identity=33.9805825242718, Blast_Score=144, Evalue=9e-35,
Organism=Homo sapiens, GI21359890, Length=289, Percent_Identity=31.8339100346021, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI20070323, Length=348, Percent_Identity=25.2873563218391, Blast_Score=121, Evalue=7e-28,
Organism=Homo sapiens, GI14210520, Length=231, Percent_Identity=29.004329004329, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI52352803, Length=132, Percent_Identity=43.9393939393939, Blast_Score=92, Evalue=9e-19,
Organism=Homo sapiens, GI52351208, Length=162, Percent_Identity=29.6296296296296, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI222080086, Length=129, Percent_Identity=27.1317829457364, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI40255009, Length=129, Percent_Identity=27.1317829457364, Blast_Score=69, Evalue=7e-12,
Organism=Escherichia coli, GI1786966, Length=313, Percent_Identity=100, Blast_Score=630, Evalue=0.0,
Organism=Escherichia coli, GI1790349, Length=276, Percent_Identity=23.9130434782609, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI193210990, Length=321, Percent_Identity=33.0218068535826, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17555794, Length=312, Percent_Identity=33.0128205128205, Blast_Score=154, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI17555796, Length=310, Percent_Identity=32.5806451612903, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI32564488, Length=303, Percent_Identity=31.023102310231, Blast_Score=134, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI17550440, Length=129, Percent_Identity=31.7829457364341, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17550442, Length=129, Percent_Identity=31.7829457364341, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320411, Length=179, Percent_Identity=35.7541899441341, Blast_Score=92, Evalue=8e-20,
Organism=Saccharomyces cerevisiae, GI6323899, Length=132, Percent_Identity=36.3636363636364, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6324892, Length=120, Percent_Identity=37.5, Blast_Score=87, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24656728, Length=346, Percent_Identity=29.1907514450867, Blast_Score=131, Evalue=7e-31,
Organism=Drosophila melanogaster, GI24656724, Length=346, Percent_Identity=29.1907514450867, Blast_Score=131, Evalue=7e-31,
Organism=Drosophila melanogaster, GI24656720, Length=346, Percent_Identity=29.1907514450867, Blast_Score=131, Evalue=7e-31,
Organism=Drosophila melanogaster, GI24656715, Length=346, Percent_Identity=29.1907514450867, Blast_Score=131, Evalue=7e-31,
Organism=Drosophila melanogaster, GI21357035, Length=346, Percent_Identity=29.1907514450867, Blast_Score=131, Evalue=7e-31,
Organism=Drosophila melanogaster, GI24583858, Length=321, Percent_Identity=28.9719626168224, Blast_Score=120, Evalue=8e-28,
Organism=Drosophila melanogaster, GI24585900, Length=125, Percent_Identity=32.8, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24585898, Length=125, Percent_Identity=32.8, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24585896, Length=125, Percent_Identity=32.8, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI19921672, Length=125, Percent_Identity=32.8, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24584409, Length=157, Percent_Identity=30.5732484076433, Blast_Score=81, Evalue=9e-16,
Organism=Drosophila melanogaster, GI28574430, Length=157, Percent_Identity=30.5732484076433, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24645863, Length=127, Percent_Identity=30.7086614173228, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24667550, Length=290, Percent_Identity=23.7931034482759, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24667546, Length=290, Percent_Identity=23.7931034482759, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ZITB_ECOLI (P75757)

Other databases:

- EMBL:   U00096
- EMBL:   AP009048
- PIR:   H64810
- RefSeq:   AP_001383.1
- RefSeq:   NP_415273.1
- ProteinModelPortal:   P75757
- STRING:   P75757
- EnsemblBacteria:   EBESCT00000002800
- EnsemblBacteria:   EBESCT00000014928
- GeneID:   945348
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW0735
- KEGG:   eco:b0752
- EchoBASE:   EB3426
- EcoGene:   EG13662
- eggNOG:   COG1230
- GeneTree:   EBGT00050000009531
- HOGENOM:   HBG317426
- OMA:   SVWIVVE
- ProtClustDB:   PRK03557
- BioCyc:   EcoCyc:B0752-MONOMER
- Genevestigator:   P75757
- HAMAP:   MF_00552
- InterPro:   IPR002524
- PANTHER:   PTHR11562
- TIGRFAMs:   TIGR01297

Pfam domain/function: PF01545 Cation_efflux

EC number: NA

Molecular weight: Translated: 34679; Mature: 34548

Theoretical pI: Translated: 6.82; Mature: 6.82

Prosite motif: PS00511 CRF

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x12fdf888)-; HASH(0x12ae0fb4)-; HASH(0x13437f14)-; HASH(0x12632620)-; HASH(0x12e8d10c)-; HASH(0x133c46bc)-;

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGSLALLADAGHMLTDTAA
CCCCCCCCHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHH
LLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVITILIVWEAIERFRTPRPVE
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHVLGDLLGSVGAIIAALIIIWT
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPVSLDIAELKRRMCREIPEVRNVH
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCCE
HVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYLMDHYQIEHATIQMEYQPCHGPDCH
EEEEEEECCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHEEEEEEEEEEECCCCCCCCC
LNEGVSGHSHHHH
CCCCCCCCCCCCH
>Mature Secondary Structure 
AHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGSLALLADAGHMLTDTAA
CCCCCCCHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHH
LLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVITILIVWEAIERFRTPRPVE
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHVLGDLLGSVGAIIAALIIIWT
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPVSLDIAELKRRMCREIPEVRNVH
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCCE
HVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYLMDHYQIEHATIQMEYQPCHGPDCH
EEEEEEECCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHEEEEEEEEEEECCCCCCCCC
LNEGVSGHSHHHH
CCCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 11443104