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The map label for this gene is dpiA

Identifier: 157160116

GI number: 157160116

Start: 689943

End: 690623

Strand: Direct

Name: dpiA

Synonym: EcHS_A0671

Alternate gene names: 157160116

Gene position: 689943-690623 (Clockwise)

Preceding gene: 157160115

Following gene: 157160118

Centisome position: 14.86

GC content: 50.37

Gene sequence:

>681_bases
ATGACAGCTCCATTAACCCTATTGATCGTTGAGGACGAAACGCCGCTGGCAGAGATGCATGCGGAATATATTCGTCACAT
TCCCGGATTCAGTCAGATATTACTGGCGGGAAACCTGGCGCAGGCCCGAATGATGATCGAGCGTTTTAAGCCGGGGCTAA
TCTTGCTCGATAACTATCTTCCTGACGGCAGAGGGATTAATTTATTGCATGAACTGGTGCAGGCGCATTATCCCGGCGAC
GTGGTGTTTACCACTGCAGCCAGCGATATGGAAACGGTGTCTGAAGCCGTACGTTGTGGTGTATTTGATTATCTCATTAA
GCCCATTGCCTATGAACGGCTGGGGCAAACGCTAACCCGTTTCCGCCAGCGTAAACATATGCTGGAAAGTATTGATAGCG
CCAGCCAGAAGCAAATTGATGAGATGTTTAATGCCTATGCTCGCGGTGAACCTAAAGACGAACTGCCGACCGGCATTGAT
CCCTTGACGCTAAACGCGGTGCGAAAACTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACT
GACCATCAGCCGCACCACTGCCAGACGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACG
GCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGA

Upstream 100 bases:

>100_bases
ATTGCCAGCTACGTAACGCGCTGCGGTGGTGTTATCACTCTCGAAGATAATGATCCCTGCGGTACCTTATTTTCAATCTA
TATTCCGAAAGTGAAACCTA

Downstream 100 bases:

>100_bases
CATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATC
AGCTCTGTAGCAACGATAAC

Product: two-component response regulator DpiA

Products: NA

Alternate protein names: Destabilizer of plasmid inheritance

Number of amino acids: Translated: 226; Mature: 225

Protein sequence:

>226_residues
MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARMMIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGD
VVFTTAASDMETVSEAVRCGVFDYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGRPQRIYHSG

Sequences:

>Translated_226_residues
MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARMMIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGD
VVFTTAASDMETVSEAVRCGVFDYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGRPQRIYHSG
>Mature_225_residues
TAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARMMIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDV
VFTTAASDMETVSEAVRCGVFDYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGIDP
LTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGRPQRIYHSG

Specific function: Member of the two-component regulatory system DpiA/DpiB, which is essential for expression of citrate-specific fermentation genes and genes involved in plasmid inheritance. Could be involved in response to both the presence of citrate and external redox c

COG id: COG4565

COG function: function code KT; Response regulator of citrate/malate metabolism

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1786838, Length=226, Percent_Identity=100, Blast_Score=466, Evalue=1e-133,
Organism=Escherichia coli, GI1790566, Length=220, Percent_Identity=31.3636363636364, Blast_Score=117, Evalue=5e-28,
Organism=Escherichia coli, GI1788550, Length=131, Percent_Identity=32.824427480916, Blast_Score=63, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DPIA_ECO57 (P0AEF6)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   C90711
- PIR:   G85561
- RefSeq:   NP_286347.1
- RefSeq:   NP_308686.1
- ProteinModelPortal:   P0AEF6
- SMR:   P0AEF6
- EnsemblBacteria:   EBESCT00000028340
- EnsemblBacteria:   EBESCT00000057901
- GeneID:   917019
- GeneID:   957649
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z0765
- KEGG:   ecs:ECs0659
- GeneTree:   EBGT00050000008649
- HOGENOM:   HBG753323
- OMA:   HAELIYG
- ProtClustDB:   PRK10046
- BioCyc:   ECOL83334:ECS0659-MONOMER
- GO:   GO:0005737
- GO:   GO:0006350
- InterPro:   IPR011006
- InterPro:   IPR001789
- SMART:   SM00448

Pfam domain/function: PF00072 Response_reg; SSF52172 CheY_like

EC number: NA

Molecular weight: Translated: 25454; Mature: 25323

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARMMIERFKPGLILLDNYL
CCCCEEEEEEECCCCHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHCCCCEEEEECCC
PDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVFDYLIKPIAYERLGQTLTR
CCCCCHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGIDPLTLNAVRKLFKEPGVQHTA
HHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHH
ETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGRPQRIYHSG
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHCCCC
>Mature Secondary Structure 
TAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARMMIERFKPGLILLDNYL
CCCEEEEEEECCCCHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHCCCCEEEEECCC
PDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVFDYLIKPIAYERLGQTLTR
CCCCCHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGIDPLTLNAVRKLFKEPGVQHTA
HHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHH
ETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGRPQRIYHSG
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796