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The map label for this gene is citC

Identifier: 157160114

GI number: 157160114

Start: 686878

End: 687936

Strand: Reverse

Name: citC

Synonym: EcHS_A0669

Alternate gene names: 157160114

Gene position: 687936-686878 (Counterclockwise)

Preceding gene: 157160117

Following gene: 157160113

Centisome position: 14.81

GC content: 50.99

Gene sequence:

>1059_bases
ATGTTCGGCAATGATATTTTCACCCGCGTAAAACGTTCAGAAAATAAAAAAATGGCGGAAATCGCCCAATTCCTGCATGA
AAATGATTTGAGCGTTGACACCACAGTCGAAGTATTTATTACCGTAACCCGCGATGAAAAGCTTATCGCGTGCGGTGGAA
TTGCCGGAAATATTATTAAATGCGTTGCTATCAGTGAATCCGTTCGCGGTGAAGGACTGGCGCTGACATTAGCCACTGAA
CTGATAAACCTCGCCTATGAGCGGCACAGCACGCATCTGTTTATTTATACCAAAACCGAATACGAGGCGCTGTTCCGCCA
GTGCGGTTTTTCCACGCTGACCAGCGTACCCGGCGTGATGGTGCTGATGGAAAACAGCGCCACGCGACTGAAACGCTATG
CCGAATCGCTGAAAAAATTTCGTCATCCAGGGAACAAGATTGGCTGCATTGTGATGAACGCCAATCCCTTTACGAATGGT
CACCGTTATCTGATTCAACAGGCTGCGGCACAGTGCGACTGGTTGCATCTGTTTTTAGTCAAAGAAGATTCTTCACGCTT
CCCCTATGAAGACCGGCTGGATCTGGTGTTAAAAGGCACCGCCGATATTCCACGCCTGACTGTGCATCGCGGCTCCGAAT
ACATCATCTCCCGCGCTACGTTCCCTTGCTACTTCATTAAAGAACAGAGCGTCATTAACCATTGTTACACCGAAATTGAT
CTGAAGATTTTCCGTCAGTACCTCGCTCCCGCACTGGGTGTAACTCACCGCTTTGTCGGTACTGAACCCTTTTGTCGCGT
TACCGCCCAGTACAACCAGGATATGCGCTACTGGCTGGAAACGCCGACTATCTCCGCACCGCCCATCGAACTGGTTGAAA
TTGAGCGGCTGCGTTACCAGGAGATGCCGATATCCGCTTCCCGGGTACGTCAACTGCTGGCGAAAAACGATCTCACGGCT
ATCGCGCCGCTGGTCCCTGCAGTCACGCTGCATTATTTGCAGAACCTGCTTGAGCACTCCCGCCAGGACGCGGCAGCTCG
TCAAAAGACCCCCGCATGA

Upstream 100 bases:

>100_bases
ACCTTTCTCCCAAGTGAAAATAAAAAGTTATCAGTTTGCAACATTGAACAACATTCGTTGCAAATCGATAACAACATGCA
CCTTCAGGATACTATTTATT

Downstream 100 bases:

>100_bases
GAAACAGGTGAAAAATGAAAATAAACCAGCCCGCCGTTGCAGGCACCCTTGAGTCTGGGGATGTGATGATACGCATCGCC
CCACTCGATACGCAGGATAT

Product: [citrate (pro-3S)-lyase] ligase

Products: NA

Alternate protein names: Acetate:SH-citrate lyase ligase; Citrate lyase synthetase

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTRDEKLIACGGIAGNIIKCVAISESVRGEGLALTLATE
LINLAYERHSTHLFIYTKTEYEALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISRATFPCYFIKEQSVINHCYTEID
LKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMRYWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTA
IAPLVPAVTLHYLQNLLEHSRQDAAARQKTPA

Sequences:

>Translated_352_residues
MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTRDEKLIACGGIAGNIIKCVAISESVRGEGLALTLATE
LINLAYERHSTHLFIYTKTEYEALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISRATFPCYFIKEQSVINHCYTEID
LKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMRYWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTA
IAPLVPAVTLHYLQNLLEHSRQDAAARQKTPA
>Mature_352_residues
MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTRDEKLIACGGIAGNIIKCVAISESVRGEGLALTLATE
LINLAYERHSTHLFIYTKTEYEALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISRATFPCYFIKEQSVINHCYTEID
LKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMRYWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTA
IAPLVPAVTLHYLQNLLEHSRQDAAARQKTPA

Specific function: Acetylation of prosthetic group (2-(5''-phosphoribosyl)- 3'-dephosphocoenzyme-A) of the gamma subunit of citrate lyase

COG id: COG3053

COG function: function code C; Citrate lyase synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 N-acetyltransferase domain

Homologues:

Organism=Escherichia coli, GI87081765, Length=352, Percent_Identity=100, Blast_Score=736, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CITC_ECOLI (P77390)

Other databases:

- EMBL:   U82598
- EMBL:   U00096
- EMBL:   AP009048
- EMBL:   U46667
- PIR:   H64795
- RefSeq:   AP_001265.1
- RefSeq:   NP_415151.4
- ProteinModelPortal:   P77390
- SMR:   P77390
- STRING:   P77390
- EnsemblBacteria:   EBESCT00000003737
- EnsemblBacteria:   EBESCT00000016685
- GeneID:   945231
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW0610
- KEGG:   eco:b0618
- EchoBASE:   EB3409
- EcoGene:   EG13645
- eggNOG:   COG3053
- GeneTree:   EBGT00050000009800
- HOGENOM:   HBG416494
- OMA:   MNCNPFT
- ProtClustDB:   CLSK874111
- BioCyc:   EcoCyc:CITC-MONOMER
- DrugBank:   DB00336
- Genevestigator:   P77390
- InterPro:   IPR000182
- InterPro:   IPR016181
- InterPro:   IPR005216
- InterPro:   IPR013166
- InterPro:   IPR004821
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.630.30
- Gene3D:   G3DSA:3.40.50.620
- PIRSF:   PIRSF005751
- SMART:   SM00764
- TIGRFAMs:   TIGR00124
- TIGRFAMs:   TIGR00125

Pfam domain/function: PF08218 Citrate_ly_lig; SSF55729 Acyl_CoA_acyltransferase

EC number: =6.2.1.22

Molecular weight: Translated: 40078; Mature: 40078

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: PS51186 GNAT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTRDEKLIACGGIAGNIIK
CCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEEEEEEECCCEEEEECCCCCCCEE
CVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYEALFRQCGFSTLTSVPGVM
EEEECCCCCCCCEEEHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHCCCHHHHCCCCEE
VLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNGHRYLIQQAAAQCDWLHLFLV
EEECCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCEEEEEEE
KEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISRATFPCYFIKEQSVINHCYTEID
ECCCCCCCCCCCCEEEEECCCCCCEEEEECCCCEEEECCCCCEEEEEHHHHHHHHHHHHH
LKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMRYWLETPTISAPPIELVEIERLRYQ
HHHHHHHHHHHHHHHHHHCCCCCCEEEEHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHH
EMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQNLLEHSRQDAAARQKTPA
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTRDEKLIACGGIAGNIIK
CCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEEEEEEECCCEEEEECCCCCCCEE
CVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYEALFRQCGFSTLTSVPGVM
EEEECCCCCCCCEEEHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHCCCHHHHCCCCEE
VLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNGHRYLIQQAAAQCDWLHLFLV
EEECCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCEEEEEEE
KEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISRATFPCYFIKEQSVINHCYTEID
ECCCCCCCCCCCCEEEEECCCCCCEEEEECCCCEEEECCCCCEEEEEHHHHHHHHHHHHH
LKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMRYWLETPTISAPPIELVEIERLRYQ
HHHHHHHHHHHHHHHHHHCCCCCCEEEEHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHH
EMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQNLLEHSRQDAAARQKTPA
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503