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The map label for this gene is citE

Identifier: 157160112

GI number: 157160112

Start: 685662

End: 686570

Strand: Reverse

Name: citE

Synonym: EcHS_A0667

Alternate gene names: 157160112

Gene position: 686570-685662 (Counterclockwise)

Preceding gene: 157160113

Following gene: 157160111

Centisome position: 14.79

GC content: 57.87

Gene sequence:

>909_bases
ATGATTTCCGCTTCGCTGCAACAACGTAAAACTCGCACCCGCCGCAGCATGTTGTTTGTGCCTGGTGCCAATGCCGCGAT
GGTCAGCAACTCCTTCATCTACCCGGCTGATGCCCTGATGTTTGACCTCGAAGACTCCGTAGCATTGCGTGAAAAAGACA
CCGCCCGCCGCATGGTTTACCACGCGCTGCAACATCCGCTGTATCGCGATATTGAAACCATTGTGCGTGTCAACGCGCTG
GATTCCGAATGGGGTGTCAACGACCTGGAAGCCGTCGTTCGCGGTGGTGCGGACGTTGTGCGTCTGCCGAAAACCGATAC
CGCTCAGGATGTTCTGGATATCGAAAAAGAGATCCTGCGTATCGAAAAAGCCTGTGGTCGTGAACCCGGCAGCACCGGCC
TGCTGGCGGCGATTGAATCTCCGCTGGGCATTACCCGCGCAGTGGAAATCGCTCACGCTTCCGAGCGTTTGATCGGTATC
GCCCTCGGTGCAGAAGACTATGTGCGCAACCTGCGTACAGAACGCTCCCCGGAAGGAACTGAACTGCTGTTCGCTCGCTG
CTCCATTTTGCAGGCCGCGCGCTCTGCGGGTATTCAGGCGTTCGATACCGTCTATTCCGACGCTAACAACGAAGCTGGAT
TCCTGCAAGAAGCCGCCCACATCAAACAGCTGGGCTTTGACGGCAAATCGCTGATCAACCCGCGTCAGATTGATCTGCTG
CACAACCTCTACGCACCGACCCAGAAAGAAGTGGATCACGCCCGCCGCGTCGTAGAAGCCGCTGAAGCCGCCGCTCGCGA
AGGCCTCGGCGTGGTTTCCCTGAACGGCAAGATGGTGGACGGTCCGGTTATCGATCGCGCCCGTCTGGTGCTCTCCCGTG
CAGAACTTTCCGGCATCCGCGAAGAATAA

Upstream 100 bases:

>100_bases
TCGATGACAAAGGCGCACTGGACTGCATTTTACGTGCACGACTGGAAGCCCTGCTGGCACGCGCCAGCGGTATCCCGGCT
CTGCCATGGGAGGATTGCCA

Downstream 100 bases:

>100_bases
GGCAATCAAAATGACGCAGAAAATTGAACAATCTCAACGACAAGAACGGGTAGCGGCCTGGAATCGTCGCGCTGAATGCG
ATCTTGCCGCTTTCCAGAAC

Product: citrate (pro-3S)-lyase, beta subunit

Products: NA

Alternate protein names: Citrase beta chain; Citrate (pro-3S)-lyase subunit beta; Citryl-CoA lyase subunit

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLEDSVALREKDTARRMVYHALQHPLYRDIETIVRVNAL
DSEWGVNDLEAVVRGGADVVRLPKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEAAHIKQLGFDGKSLINPRQIDLL
HNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNGKMVDGPVIDRARLVLSRAELSGIREE

Sequences:

>Translated_302_residues
MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLEDSVALREKDTARRMVYHALQHPLYRDIETIVRVNAL
DSEWGVNDLEAVVRGGADVVRLPKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEAAHIKQLGFDGKSLINPRQIDLL
HNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNGKMVDGPVIDRARLVLSRAELSGIREE
>Mature_302_residues
MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLEDSVALREKDTARRMVYHALQHPLYRDIETIVRVNAL
DSEWGVNDLEAVVRGGADVVRLPKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEAAHIKQLGFDGKSLINPRQIDLL
HNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNGKMVDGPVIDRARLVLSRAELSGIREE

Specific function: Represents a citryl-ACP lyase

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family. Citrate lyase beta subunit subfamily

Homologues:

Organism=Homo sapiens, GI45545437, Length=310, Percent_Identity=25.1612903225806, Blast_Score=109, Evalue=4e-24,
Organism=Escherichia coli, GI87081764, Length=302, Percent_Identity=100, Blast_Score=610, Evalue=1e-176,
Organism=Caenorhabditis elegans, GI17557458, Length=301, Percent_Identity=27.906976744186, Blast_Score=107, Evalue=9e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CITE_ECOL6 (P0A9I2)

Other databases:

- EMBL:   AE014075
- RefSeq:   NP_752637.1
- ProteinModelPortal:   P0A9I2
- SMR:   P0A9I2
- PRIDE:   P0A9I2
- EnsemblBacteria:   EBESCT00000041243
- GeneID:   1035930
- GenomeReviews:   AE014075_GR
- KEGG:   ecc:c0706
- GeneTree:   EBGT00050000010557
- HOGENOM:   HBG676713
- OMA:   DANNEEG
- ProtClustDB:   CLSK874112
- BRENDA:   4.1.3.34
- BRENDA:   4.1.3.6
- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR006475
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PIRSF:   PIRSF015582
- TIGRFAMs:   TIGR01588

Pfam domain/function: PF03328 HpcH_HpaI; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =4.1.3.6; =4.1.3.34

Molecular weight: Translated: 33110; Mature: 33110

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: NA

Important sites: BINDING 76-76 BINDING 139-139

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLEDSVALREKDTARRMVY
CCCCHHHHHHHHHHHCEEEECCCCHHHHCCCCCCCCHHHEEECCCCHHHHHHHHHHHHHH
HALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRLPKTDTAQDVLDIEKEILR
HHHHCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHH
IEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGIALGAEDYVRNLRTERSPEGT
HHHHHCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHEEEEEECHHHHHHHHHHCCCCCHH
ELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEAAHIKQLGFDGKSLINPRQIDLL
HHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHCCCHHHHHH
HNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNGKMVDGPVIDRARLVLSRAELSGIR
HHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEECCCHHHHHHHHHHHHHHHCCC
EE
CC
>Mature Secondary Structure
MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLEDSVALREKDTARRMVY
CCCCHHHHHHHHHHHCEEEECCCCHHHHCCCCCCCCHHHEEECCCCHHHHHHHHHHHHHH
HALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRLPKTDTAQDVLDIEKEILR
HHHHCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHH
IEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGIALGAEDYVRNLRTERSPEGT
HHHHHCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHEEEEEECHHHHHHHHHHCCCCCHH
ELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEAAHIKQLGFDGKSLINPRQIDLL
HHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHCCCHHHHHH
HNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNGKMVDGPVIDRARLVLSRAELSGIR
HHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEECCCHHHHHHHHHHHHHHHCCC
EE
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157