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The map label for this gene is cusS [H]

Identifier: 157160062

GI number: 157160062

Start: 629416

End: 630864

Strand: Reverse

Name: cusS [H]

Synonym: EcHS_A0617

Alternate gene names: 157160062

Gene position: 630864-629416 (Counterclockwise)

Preceding gene: 157160063

Following gene: 157160061

Centisome position: 13.59

GC content: 51.9

Gene sequence:

>1449_bases
ATGGTCAGTAAGCCATTTCAGCGCCCGTTTTCGCTGGCAACCCGCCTGACCTTTTTTATCAGCCTGGCCACCATCGCGGC
GTTTTTCGCCTTTGCATGGATCATGATCCACTCAGTAAAAGTTCATTTTGCCGAGCAGGATATTAATGATTTAAAAGAGA
TTAGCGCCACCCTTGAACGGGTACTAAATCACCCTGACGAAACGCAAGCCCGACGCTTAATGACGCTGGAAGATATCGTC
AGTGGTTATTCCAATGTGTTGATTTCCCTGGCAGATAGTCAGGGTAAAACGGTGTATCACTCCCCCGGTGCGCCGGATAT
CCGCGAGTTTACGCGTGACGCCATACCCGATAAAGACGCTCAGGGTGGCGAGGTGTATCTCCTTTCCGGCCCGACGATGA
TGATGCCAGGCCACGGTCACGGGCATATGGAACACAGCAACTGGCGGATGATTAACTTGCCGGTTGGCCCGTTGGTGGAC
GGCAAACCGATTTATACGCTCTACATCGCGCTTTCGATCGATTTTCATCTTCATTACATAAATGATTTGATGAATAAACT
TATTATGACCGCATCGGTAATCAGCATCCTGATCGTCTTTATCGTACTGTTGGCGGTACATAAAGGTCACGCGCCGATCC
GCAGCGTCAGCCGTCAAATCCAGAATATTACCTCGAAAGATCTCGACGTTCGCCTCGACCCGCAGACCGTGCCGATTGAG
CTGGAACAGCTGGTACTGTCGTTCAACCATATGATCGAGCGTATTGAGGATGTCTTTACCCGCCAGTCCAATTTCTCAGC
GGATATCGCTCACGAAATTCGCACGCCGATTACCAATCTCATCACGCAAACGGAAATCGCCCTCAGCCAGTCTCGCAGCC
AGAAGGAGCTGGAAGATGTGCTCTACTCTAATCTCGAAGAGCTGACGCGAATGGCGAAAATGGTCAGCGATATGCTGTTT
CTCGCTCAGGCCGATAACAACCAGCTAATCCCCGAAAAGAAAATGCTCAACCTGGCGGATGAAGTCGGCAAAGTGTTCGA
TTTTTTCGAGGCGTTAGCGGAAGATCGCGGCGTGGAGTTGCGATTTGTTGGCGACAAATGTCAGGTTGCGGGCGATCCGC
TGATGCTGCGTCGGGCGTTAAGCAACCTGCTTTCTAATGCCCTGCGTTATACGCCACCCAGTGAGGCTATTGTAGTGCGC
TGCCAGACGGTCAATCATCAGGTGCAAGTTTCCGTCGAAAACCCCGGTACGCCCATTGCGCCCGAGCACTTACCGCGATT
GTTTGACCGTTTCTATCGCGTTGACCCTTCCCGCCAGCGAAAAGGTGAAGGTAGCGGTATTGGGCTGGCGATAGTGAAAT
CGATTGTTGTCGCGCATAAAGGCACGGTTGCGGTAACGTCAGATGCGCGGGGGACAAGGTTTGTGATTATGCTGCCGGAG
AGAGAGTGA

Upstream 100 bases:

>100_bases
GATGTGGCGGTGAAGCGGCTGCGCGGCAAAATCGACAACGACTTTGAGCCGAAGCTAATTCAGACCGTGCGCGGCGTGGG
TTACATGCTTGAGGTGCCGG

Downstream 100 bases:

>100_bases
TTATTAAGTTCAAATTCAGGGCGTGGTGCATCACCTCCTGGTATTGTTGATCAACAAATTAGAGAAAAACTTATTAAACA
TATGAAATATCGAAGGCTTT

Product: sensor kinase CusS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFAEQDINDLKEISATLERVLNHPDETQARRLMTLEDIV
SGYSNVLISLADSQGKTVYHSPGAPDIREFTRDAIPDKDAQGGEVYLLSGPTMMMPGHGHGHMEHSNWRMINLPVGPLVD
GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSRQIQNITSKDLDVRLDPQTVPIE
LEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTPITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLF
LAQADNNQLIPEKKMLNLADEVGKVFDFFEALAEDRGVELRFVGDKCQVAGDPLMLRRALSNLLSNALRYTPPSEAIVVR
CQTVNHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLAIVKSIVVAHKGTVAVTSDARGTRFVIMLPE
RE

Sequences:

>Translated_482_residues
MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFAEQDINDLKEISATLERVLNHPDETQARRLMTLEDIV
SGYSNVLISLADSQGKTVYHSPGAPDIREFTRDAIPDKDAQGGEVYLLSGPTMMMPGHGHGHMEHSNWRMINLPVGPLVD
GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSRQIQNITSKDLDVRLDPQTVPIE
LEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTPITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLF
LAQADNNQLIPEKKMLNLADEVGKVFDFFEALAEDRGVELRFVGDKCQVAGDPLMLRRALSNLLSNALRYTPPSEAIVVR
CQTVNHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLAIVKSIVVAHKGTVAVTSDARGTRFVIMLPE
RE
>Mature_482_residues
MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFAEQDINDLKEISATLERVLNHPDETQARRLMTLEDIV
SGYSNVLISLADSQGKTVYHSPGAPDIREFTRDAIPDKDAQGGEVYLLSGPTMMMPGHGHGHMEHSNWRMINLPVGPLVD
GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSRQIQNITSKDLDVRLDPQTVPIE
LEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTPITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLF
LAQADNNQLIPEKKMLNLADEVGKVFDFFEALAEDRGVELRFVGDKCQVAGDPLMLRRALSNLLSNALRYTPPSEAIVVR
CQTVNHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLAIVKSIVVAHKGTVAVTSDARGTRFVIMLPE
RE

Specific function: Member of the two-component regulatory system CusS/CusR. Copper ion sensor. Could also be a silver ion sensor. Probably activates CusR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786783, Length=479, Percent_Identity=98.5386221294363, Blast_Score=969, Evalue=0.0,
Organism=Escherichia coli, GI1788279, Length=296, Percent_Identity=33.445945945946, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1786600, Length=265, Percent_Identity=33.9622641509434, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1790346, Length=332, Percent_Identity=29.5180722891566, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1788393, Length=275, Percent_Identity=29.8181818181818, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1787894, Length=311, Percent_Identity=29.2604501607717, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1789403, Length=282, Percent_Identity=30.8510638297872, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI145693157, Length=237, Percent_Identity=26.5822784810127, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1789808, Length=283, Percent_Identity=27.5618374558304, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1789149, Length=236, Percent_Identity=27.5423728813559, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790436, Length=220, Percent_Identity=26.3636363636364, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790551, Length=305, Percent_Identity=27.2131147540984, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI87081816, Length=253, Percent_Identity=27.2727272727273, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788713, Length=232, Percent_Identity=28.0172413793103, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1790861, Length=298, Percent_Identity=25.503355704698, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI87082128, Length=295, Percent_Identity=24.7457627118644, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787374, Length=340, Percent_Identity=22.0588235294118, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1786912, Length=244, Percent_Identity=25.4098360655738, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI48994928, Length=237, Percent_Identity=24.0506329113924, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 54096; Mature: 54096

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFAEQDINDLKEISATLER
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHHHHHHHH
VLNHPDETQARRLMTLEDIVSGYSNVLISLADSQGKTVYHSPGAPDIREFTRDAIPDKDA
HHCCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEECCCCCHHHHHHHHCCCCCCC
QGGEVYLLSGPTMMMPGHGHGHMEHSNWRMINLPVGPLVDGKPIYTLYIALSIDFHLHYI
CCCEEEEEECCEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEEEEEEEHHHHHH
NDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSRQIQNITSKDLDVRLDPQTVPIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCHH
LEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTPITNLITQTEIALSQSRSQKELEDV
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
LYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKKMLNLADEVGKVFDFFEALAEDRGVEL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
RFVGDKCQVAGDPLMLRRALSNLLSNALRYTPPSEAIVVRCQTVNHQVQVSVENPGTPIA
EEECCHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCEEEEEECCCCCCCC
PEHLPRLFDRFYRVDPSRQRKGEGSGIGLAIVKSIVVAHKGTVAVTSDARGTRFVIMLPE
HHHHHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEECC
RE
CC
>Mature Secondary Structure
MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFAEQDINDLKEISATLER
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHHHHHHHH
VLNHPDETQARRLMTLEDIVSGYSNVLISLADSQGKTVYHSPGAPDIREFTRDAIPDKDA
HHCCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEECCCCCHHHHHHHHCCCCCCC
QGGEVYLLSGPTMMMPGHGHGHMEHSNWRMINLPVGPLVDGKPIYTLYIALSIDFHLHYI
CCCEEEEEECCEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEEEEEEEHHHHHH
NDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSRQIQNITSKDLDVRLDPQTVPIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCHH
LEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTPITNLITQTEIALSQSRSQKELEDV
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
LYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKKMLNLADEVGKVFDFFEALAEDRGVEL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
RFVGDKCQVAGDPLMLRRALSNLLSNALRYTPPSEAIVVRCQTVNHQVQVSVENPGTPIA
EEECCHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCEEEEEECCCCCCCC
PEHLPRLFDRFYRVDPSRQRKGEGSGIGLAIVKSIVVAHKGTVAVTSDARGTRFVIMLPE
HHHHHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEECC
RE
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]