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The map label for this gene is fsr

Identifier: 157160006

GI number: 157160006

Start: 569541

End: 570761

Strand: Reverse

Name: fsr

Synonym: EcHS_A0556

Alternate gene names: 157160006

Gene position: 570761-569541 (Counterclockwise)

Preceding gene: 157160008

Following gene: 157160005

Centisome position: 12.29

GC content: 52.33

Gene sequence:

>1221_bases
ATGGCAATGAGTGAACAACCCCAGCCTGTGGCGGGCGCGGCTGCGTCAACGACCAAGGCCCGAACATCGTTTGGTATTTT
AGGTGCTATCAGCCTCTCACATCTGCTGAACGACATGATCCAATCGCTGATTCTGGCGATTTATCCGCTGCTTCAGTCAG
AATTTTCTCTGACATTTATGCAGATTGGCATGATAACCCTCACCTTCCAGCTCGCCTCTTCGCTACTGCAACCAGTGGTC
GGCTACTGGACCGATAAATATCCGATGCCGTGGTCGTTGCCAATTGGCATGTGCTTTACCTTAAGTGGTCTGGTGCTGCT
TGCGCTGGCGGGCAGTTTTGGCGCAGTTCTGCTGGCGGCGGCGCTGGTCGGTACCGGTTCATCGGTCTTTCATCCGGAAT
CTTCTCGCGTGGCCCGTATGGCTTCCGGCGGGCGGCATGGCCTGGCACAATCTATCTTTCAGGTCGGCGGCAACTTTGGC
AGTTCCCTGGGACCCTTGCTGGCGGCGGTGATTATCGCGCCTTATGGCAAAGGCAACGTTGCCTGGTTTGTGCTTGCGGC
ACTGCTGGCGATCGTGGTGTTGGCACAAATCAGCCGTTGGTACTCGGCACAGCACCGAATGAATAAAGGAAAACCCAAAG
CGACGATAATCAATCCACTGCCGCGCAACAAAGTGGTACTGGCAGTCAGCATTCTGTTAATCCTCATTTTCTCGAAATAT
TTCTATATGGCGAGCATCAGCAGCTATTACACCTTTTATCTGATGCAAAAATTCGGATTATCTATCCAGAATGCCCAGCT
TCATCTGTTTGCCTTCCTGTTTGCCGTTGCGGCAGGTACGGTGATCGGCGGGCCTGTAGGGGATAAAATTGGACGGAAAT
ATGTGATTTGGGGCTCTATCCTCGGCGTTGCGCCGTTTACGCTGATTTTACCCTACGCCAGCCTGCACTGGACGGGGGTT
TTAACGGTGATTATTGGATTTATCCTCGCTTCGGCATTCTCTGCCATTCTGGTCTACGCTCAGGAGCTACTTCCGGGACG
TATCGGTATGGTTTCTGGACTCTTTTTCGGTTTTGCTTTTGGCATGGGAGGTCTGGGAGCGGCAGTTCTGGGGCTTATCG
CCGATCACACCAGCATCGAGTTAGTCTATAAAATCTGTGCTTTCCTGCCACTATTGGGGATGTTGACCATATTCCTGCCT
GATAACCGGCATAAAGACTGA

Upstream 100 bases:

>100_bases
TTGAGACTTCCTTCAGACGACGCATACAGGTATGTTAGTCGGGTAATAATCACAAATAAGATCATTCTTTACATCGCAAC
CCTGAAAAAGTGACTTTCCT

Downstream 100 bases:

>100_bases
TTTCATTGCAGCCAAAGGCAAACTTTGGCTGCATCGTTTTACAGTCGCCATAAGCCTTTCCTCTGTTAAACCGCCTTCTG
TTTTCCGATCCGTGCTTTTT

Product: fosmidomycin resistance protein

Products: fosmidomycin [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLILAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVV
GYWTDKYPMPWSLPIGMCFTLSGLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIINPLPRNKVVLAVSILLILIFSKY
FYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAVAAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGV
LTVIIGFILASAFSAILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLLGMLTIFLP
DNRHKD

Sequences:

>Translated_406_residues
MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLILAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVV
GYWTDKYPMPWSLPIGMCFTLSGLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIINPLPRNKVVLAVSILLILIFSKY
FYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAVAAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGV
LTVIIGFILASAFSAILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLLGMLTIFLP
DNRHKD
>Mature_405_residues
AMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLILAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVG
YWTDKYPMPWSLPIGMCFTLSGLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFGS
SLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIINPLPRNKVVLAVSILLILIFSKYF
YMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAVAAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVL
TVIIGFILASAFSAILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLLGMLTIFLPD
NRHKD

Specific function: Confers the resistance against fosmidomycin

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily

Homologues:

Organism=Escherichia coli, GI1786686, Length=406, Percent_Identity=100, Blast_Score=803, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FSR_ECOLI (P52067)

Other databases:

- EMBL:   D73370
- EMBL:   U82664
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   JC5041
- RefSeq:   AP_001128.1
- RefSeq:   NP_415012.1
- ProteinModelPortal:   P52067
- STRING:   P52067
- EnsemblBacteria:   EBESCT00000001404
- EnsemblBacteria:   EBESCT00000015792
- GeneID:   945119
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW0468
- KEGG:   eco:b0479
- EchoBASE:   EB3054
- EcoGene:   EG13268
- eggNOG:   COG0477
- GeneTree:   EBGT00050000008828
- HOGENOM:   HBG611985
- OMA:   NLFWTGV
- ProtClustDB:   CLSK863544
- BioCyc:   EcoCyc:FSR-MONOMER
- Genevestigator:   P52067
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196

Pfam domain/function: PF07690 MFS_1; SSF103473 MFS_gen_substrate_transporter

EC number: NA

Molecular weight: Translated: 43306; Mature: 43175

Theoretical pI: Translated: 10.09; Mature: 10.09

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x13173f9c)-; HASH(0x12f8b30c)-; HASH(0x130f0f34)-; HASH(0x13072594)-; HASH(0x11851c80)-; HASH(0x1304a9d4)-; HASH(0x12f465ac)-; HASH(0x130c7fd4)-; HASH(0x11aef4b4)-; HASH(0x12c9b4c8)-; HASH(0x124f091c)-;

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLILAIYPLLQSEFSLTFM
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLSGLVLLALAGSFGAVLLAA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFGSSLGPLLAAVIIAPYGKGNV
HHHCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCH
AWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIINPLPRNKVVLAVSILLILIFSKY
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHH
FYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAVAAGTVIGGPVGDKIGRKYVIWGSI
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHCCEEEEEHHH
LGVAPFTLILPYASLHWTGVLTVIIGFILASAFSAILVYAQELLPGRIGMVSGLFFGFAF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
GMGGLGAAVLGLIADHTSIELVYKICAFLPLLGMLTIFLPDNRHKD
CCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
>Mature Secondary Structure 
AMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLILAIYPLLQSEFSLTFM
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLSGLVLLALAGSFGAVLLAA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFGSSLGPLLAAVIIAPYGKGNV
HHHCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCH
AWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIINPLPRNKVVLAVSILLILIFSKY
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHH
FYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAVAAGTVIGGPVGDKIGRKYVIWGSI
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHCCEEEEEHHH
LGVAPFTLILPYASLHWTGVLTVIIGFILASAFSAILVYAQELLPGRIGMVSGLFFGFAF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
GMGGLGAAVLGLIADHTSIELVYKICAFLPLLGMLTIFLPDNRHKD
CCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: fosmidomycin [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: fosmidomycin [Cytoplasm] + Proton [Periplasm] = fosmidomycin [Periplasm] + Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8917080; 9278503