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The map label for this gene is aes [H]

Identifier: 157160003

GI number: 157160003

Start: 565079

End: 566038

Strand: Reverse

Name: aes [H]

Synonym: EcHS_A0553

Alternate gene names: 157160003

Gene position: 566038-565079 (Counterclockwise)

Preceding gene: 157160005

Following gene: 157159994

Centisome position: 12.19

GC content: 52.08

Gene sequence:

>960_bases
ATGAAGCCGGAAAACAAATCACCTGTTCTGGACCTTATTTCTGCTGGAATGAAGACCGTTGTGAATACTCTTCAGCCGGA
TTTACCGCCCTGGCCCGCAACGGGAACGATTGCTGAGCAACGACAGTATTACACGCTTGAGCGCCGATTCTGGAATGCGG
GCGCTCCAGAAATGGCAACCAGAGCTTACATGGTTCCAACAAAATATGGGCAGGTGGAAACACGTCTCTTTTGTCCGCAG
CCAGATAGCCCGGCGACGCTATTTTATTTGCATGGAGGCGGTTTTATTCTCGGCAATCTCGATACCCACGATCGCATCAT
GCGCCTGCTGGCAAGCTACAGCCAATGTACGGTGATTGGTATTGATTACACCCTTTCACCTGAAGCGCGTTTTCCGCAAG
CGATAGAGGAAATTGTGGCTGCTTGTTGTTATTTCCACCAGCAGGCGGAGGATTATCAAATCAATATGTCCCGCATTGGC
TTTGCCGGTGATTCCGCAGGTGCCATGCTGGCGCTCGCCAGTGCGTTGTGGTTGCGTGATAAACAGATCGATTGCGGTAA
AATTGCGGGTGTTTTGCTGTGGTATGGGCTTTACGGATTACGGGATTCCGTGACTCGTCGTCTGTTGGGCGGTGTCTGGG
ATGGCTTAACGCAACAGGATTTGCAGATGTATGAAGAGGCGTATTTAAGCAACGACGCGGACCGCGAGTCGCCGTATTAC
TGTCTGTTTAATAATGATCTCACTCGCGAAGTTCCGCCCTGTTTTATTGCCGGGGCGGAGTTCGATCCGCTGCTGGATGA
CAGCCGTCTGCTTTACCAGACGTTAGCGGCGCATCAGCAGCCCTGTGAGTTCAAACTCTACCCAGGCACGCTGCACGCCT
TTTTGCATTATTCACGGATGATGAAAACCGCCGACGAGGCTCTTCGCGACGGCGCTCAGTTCTTTACCGCTCAGCTTTAG

Upstream 100 bases:

>100_bases
GGGAGCGCGCGAGACGCAGCCAGTTTCAATATCAGCAGGCGGGATCGCATTTTCATGGCAGTGGTTCCTTACAATGACGT
AATTTGAAAGGAGTTTTTGT

Downstream 100 bases:

>100_bases
CGATACGCGGCAACAAGATTAGCCATCATTTCAATATGTTCCGGCGTGGCATTAAGCGCTGGAATATATTCATATTTTTT
CCCGCCGGCACCGAGGAAGA

Product: acetyl esterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MKPENKSPVLDLISAGMKTVVNTLQPDLPPWPATGTIAEQRQYYTLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQ
PDSPATLFYLHGGGFILGNLDTHDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQQDLQMYEEAYLSNDADRESPYY
CLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLAAHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL

Sequences:

>Translated_319_residues
MKPENKSPVLDLISAGMKTVVNTLQPDLPPWPATGTIAEQRQYYTLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQ
PDSPATLFYLHGGGFILGNLDTHDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQQDLQMYEEAYLSNDADRESPYY
CLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLAAHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL
>Mature_319_residues
MKPENKSPVLDLISAGMKTVVNTLQPDLPPWPATGTIAEQRQYYTLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQ
PDSPATLFYLHGGGFILGNLDTHDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQQDLQMYEEAYLSNDADRESPYY
CLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLAAHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL

Specific function: Displays esterase activity towards short chain fatty esters (acyl chain length of up to 8 carbons). Able to hydrolyze triacetylglycerol (triacetin) and tributyrylglycerol (tributyrin), but not trioleylglycerol (triolein) or cholesterol oleate. Negatively

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI68299767, Length=132, Percent_Identity=32.5757575757576, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1786682, Length=319, Percent_Identity=99.0595611285266, Blast_Score=659, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17540028, Length=142, Percent_Identity=33.0985915492958, Blast_Score=75, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI71996133, Length=266, Percent_Identity=25.187969924812, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI72001146, Length=130, Percent_Identity=32.3076923076923, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24656084, Length=98, Percent_Identity=39.7959183673469, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24656076, Length=98, Percent_Identity=39.7959183673469, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI20130169, Length=98, Percent_Identity=39.7959183673469, Blast_Score=81, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.-

Molecular weight: Translated: 35950; Mature: 35950

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS01173 LIPASE_GDXG_HIS ; PS01174 LIPASE_GDXG_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPENKSPVLDLISAGMKTVVNTLQPDLPPWPATGTIAEQRQYYTLERRFWNAGAPEMAT
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHH
RAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDTHDRIMRLLASYSQCTVIG
EEEECCCCCCCEEEEEECCCCCCCCEEEEEECCEEEEECCCHHHHHHHHHHCCCCEEEEE
IDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIGFAGDSAGAMLALASALWLRD
EEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEHHHCCCCCCCCHHHHHHHHHHHHCC
KQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQQDLQMYEEAYLSNDADRESPYY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCEE
CLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLAAHQQPCEFKLYPGTLHAFLHYSRM
EEECCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHH
MKTADEALRDGAQFFTAQL
HHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKPENKSPVLDLISAGMKTVVNTLQPDLPPWPATGTIAEQRQYYTLERRFWNAGAPEMAT
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHH
RAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDTHDRIMRLLASYSQCTVIG
EEEECCCCCCCEEEEEECCCCCCCCEEEEEECCEEEEECCCHHHHHHHHHHCCCCEEEEE
IDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIGFAGDSAGAMLALASALWLRD
EEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEHHHCCCCCCCCHHHHHHHHHHHHCC
KQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQQDLQMYEEAYLSNDADRESPYY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCEE
CLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLAAHQQPCEFKLYPGTLHAFLHYSRM
EEECCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHH
MKTADEALRDGAQFFTAQL
HHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA