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The map label for this gene is acrA

Identifier: 157159989

GI number: 157159989

Start: 550491

End: 551684

Strand: Reverse

Name: acrA

Synonym: EcHS_A0539

Alternate gene names: 157159989

Gene position: 551684-550491 (Counterclockwise)

Preceding gene: 157159993

Following gene: 157159988

Centisome position: 11.88

GC content: 53.85

Gene sequence:

>1194_bases
ATGAACAAAAACAGAGGGTTTACGCCTCTGGCGGTCGTTCTGATGCTCTCAGGCAGCTTAGCCCTAACAGGATGTGACGA
CAAACAGGCCCAACAAGGTGGCCAGCAGATGCCCGCCGTTGGCGTAGTAACAGTCAAAACTGAACCTCTGCAGATCACAA
CCGAGCTTCCGGGTCGCACCAGTGCCTACCGGATCGCAGAAGTTCGTCCTCAAGTTAGCGGGATTATCCTGAAGCGTAAT
TTCAAAGAAGGTAGCGACATCGAAGCAGGTGTCTCTCTCTATCAGATTGATCCTGCGACCTATCAGGCGACATACGACAG
TGCGAAAGGTGATCTGGCGAAAGCCCAGGCTGCAGCCAATATCGCGCAATTGACGGTGAATCGTTATCAGAAACTGCTCG
GTACTCAGTACATCAGTAAGCAAGAGTACGATCAGGCTCTGGCTGATGCGCAACAGGCGAATGCTGCGGTAACTGCGGCG
AAAGCTGCCGTTGAAACTGCGCGGATCAATCTGGCTTACACCAAAGTCACCTCTCCGATTAGCGGTCGCATTGGTAAGTC
GAACGTGACGGAAGGCGCATTGGTACAGAACGGTCAGGCGACTGCGCTGGCAACCGTGCAGCAACTTGATCCGATCTACG
TTGATGTGACCCAGTCCAGCAACGACTTCCTGCGCCTGAAACAGGAACTGGCGAATGGCACGCTGAAACAAGAGAACGGC
AAAGCCAAAGTGTCACTGATCACCAGTGACGGCATTAAGTTCCCGCAGGACGGTACGCTGGAATTCTCTGACGTTACCGT
TGATCAGACCACTGGGTCTATCACCCTACGCGCTATCTTCCCGAACCCGGATCACACTCTGCTGCCGGGTATGTTCGTGC
GCGCACGTCTGGAAGAAGGGCTTAATCCAAACGCTATTTTAGTCCCGCAACAGGGCGTAACCCGTACGCCGCGTGGCGAT
GCCACCGTACTGGTAGTTGGCGCGGATGACAAAGTGGAAACCCGTCCGATCGTTGCAAGCCAGGCTATTGGCGATAAGTG
GCTGGTGACAGAAGGTCTGAAAGCAGGCGATCGCGTAGTAATAAGTGGGCTGCAGAAAGTGCGTCCTGGTGTCCAGGTAA
AAGCACAAGAAGTTACCGCTGATAATAACCAGCAAGCCGCAAGCGGTGCTCAGCCTGAACAGTCCAAGTCTTAA

Upstream 100 bases:

>100_bases
GAATGTATGTACCATAGCACGACGATAATATAAACGCAGCAATGGGTTTATTAACTTTTGACCATTGACCAATTTGAAAT
CGGACACTCGAGGTTTACAT

Downstream 100 bases:

>100_bases
CTTAAACAGGAGCCGTTAAGACATGCCTAATTTCTTTATCGATCGCCCGATTTTTGCGTGGGTGATCGCCATTATCATCA
TGTTGGCAGGGGGGCTGGCG

Product: acriflavine resistance protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVTVKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRN
FKEGSDIEAGVSLYQIDPATYQATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQSSNDFLRLKQELANGTLKQENG
KAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITLRAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGD
ATVLVVGADDKVETRPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQPEQSKS

Sequences:

>Translated_397_residues
MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVTVKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRN
FKEGSDIEAGVSLYQIDPATYQATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQSSNDFLRLKQELANGTLKQENG
KAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITLRAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGD
ATVLVVGADDKVETRPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQPEQSKS
>Mature_397_residues
MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVTVKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRN
FKEGSDIEAGVSLYQIDPATYQATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQSSNDFLRLKQELANGTLKQENG
KAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITLRAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGD
ATVLVVGADDKVETRPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQPEQSKS

Specific function: AcrAB is a drug efflux protein with a broad substrate specificity

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family

Homologues:

Organism=Escherichia coli, GI1786668, Length=397, Percent_Identity=100, Blast_Score=798, Evalue=0.0,
Organism=Escherichia coli, GI1789665, Length=375, Percent_Identity=69.3333333333333, Blast_Score=514, Evalue=1e-147,
Organism=Escherichia coli, GI1789929, Length=382, Percent_Identity=54.4502617801047, Blast_Score=407, Evalue=1e-115,
Organism=Escherichia coli, GI87082036, Length=357, Percent_Identity=29.1316526610644, Blast_Score=138, Evalue=6e-34,
Organism=Escherichia coli, GI87081951, Length=117, Percent_Identity=35.042735042735, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ACRA_ECO57 (P0AE07)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   D90693
- PIR:   H85543
- RefSeq:   NP_286204.1
- RefSeq:   NP_308543.1
- ProteinModelPortal:   P0AE07
- SMR:   P0AE07
- PRIDE:   P0AE07
- EnsemblBacteria:   EBESCT00000024772
- EnsemblBacteria:   EBESCT00000056056
- GeneID:   914620
- GeneID:   957439
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z0578
- KEGG:   ecs:ECs0516
- GeneTree:   EBGT00050000009284
- HOGENOM:   HBG742930
- OMA:   PQAFTLT
- ProtClustDB:   PRK15030
- BioCyc:   ECOL83334:ECS0516-MONOMER
- InterPro:   IPR006143
- TIGRFAMs:   TIGR01730

Pfam domain/function: PF00529 HlyD

EC number: NA

Molecular weight: Translated: 42197; Mature: 42197

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: PS51257 PROKAR_LIPOPROTEIN; PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVTVKTEPLQITTELPGRT
CCCCCCCCHHHHHHHHCCCEEEECCCHHHHHCCCCCCCEEEEEEEECCCEEEEECCCCCC
SAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQATYDSAKGDLAKAQAAAN
CEEEEEECCCCCEEEEEEECCCCCCCCHHCEEEEEECCCEEEEECCCCCCCHHHHHHHHH
IAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAAKAAVETARINLAYTKVTSPI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHEEEEEEEEEEEECCCC
SGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQSSNDFLRLKQELANGTLKQENG
CCCCCCCCCCCCCEEECCCCCEEHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCEECCCC
KAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITLRAIFPNPDHTLLPGMFVRARLEEG
CEEEEEEECCCCCCCCCCCEEEEEEEEECCCCEEEEEEECCCCCCCCCCHHHHHHHHHCC
LNPNAILVPQQGVTRTPRGDATVLVVGADDKVETRPIVASQAIGDKWLVTEGLKAGDRVV
CCCCEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCEEEHHCCCCCEEEECCCCCCCHHH
ISGLQKVRPGVQVKAQEVTADNNQQAASGAQPEQSKS
HHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVTVKTEPLQITTELPGRT
CCCCCCCCHHHHHHHHCCCEEEECCCHHHHHCCCCCCCEEEEEEEECCCEEEEECCCCCC
SAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQATYDSAKGDLAKAQAAAN
CEEEEEECCCCCEEEEEEECCCCCCCCHHCEEEEEECCCEEEEECCCCCCCHHHHHHHHH
IAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAAKAAVETARINLAYTKVTSPI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHEEEEEEEEEEEECCCC
SGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQSSNDFLRLKQELANGTLKQENG
CCCCCCCCCCCCCEEECCCCCEEHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCEECCCC
KAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITLRAIFPNPDHTLLPGMFVRARLEEG
CEEEEEEECCCCCCCCCCCEEEEEEEEECCCCEEEEEEECCCCCCCCCCHHHHHHHHHCC
LNPNAILVPQQGVTRTPRGDATVLVVGADDKVETRPIVASQAIGDKWLVTEGLKAGDRVV
CCCCEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCEEEHHCCCCCEEEECCCCCCCHHH
ISGLQKVRPGVQVKAQEVTADNNQQAASGAQPEQSKS
HHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796