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The map label for this gene is ddl

Identifier: 157159898

GI number: 157159898

Start: 463726

End: 464820

Strand: Reverse

Name: ddl

Synonym: EcHS_A0447

Alternate gene names: 157159898

Gene position: 464820-463726 (Counterclockwise)

Preceding gene: 157159905

Following gene: 157159896

Centisome position: 10.01

GC content: 51.32

Gene sequence:

>1095_bases
ATGGAAAAACTGCGGGTAGGAATCGTTTTTGGTGGTAAATCAGCGGAACATGAAGTGTCTCTGCAATCGGCAAAAAACAT
TGTCGATGCCATTGATAAAAGTCGCTTCGACGTTGTGCTGCTGGGCATTGATAAACAAGGGCAATGGCACGTCAGCGATG
CCAGCAATTATCTGCTAAATGCAGACGATCCTGCCCATATTGCGTTGCGCCCTTCGGCGACCAGCCTTGCGCAGGTGCCA
GGTAAACATGAGCATCAGCTTATCGACGCGCAAAACGGTCAGCCGTTGCCGACGGTGGATGTCATTTTCCCGATTGTCCA
CGGTACGCTGGGCGAAGATGGTTCCTTGCAGGGAATGCTGCGGGTCGCCAATTTACCGTTTGTAGGTTCTGATGTTCTGG
CTTCAGCAGCCTGTATGGATAAAGATGTCACCAAACGTCTGCTACGCGATGCCGGGCTGAACATTGCGCCATTTATTACC
CTGACGCGCGCTAATCGTCACAACATCAGTTTTGCCGAAGTGGAGTCTAAACTGGGGTTACCACTGTTTGTAAAACCGGC
TAATCAGGGCTCTTCTGTTGGTGTCAGCAAAGTAACCAGTGAAGAACAGTACGCAATTGCCGTCGATCTGGCGTTCGAGT
TCGATCATAAAGTGATCGTTGAGCAAGGGATCAAAGGTCGTGAGATCGAATGCGCAGTTCTGGGCAACGACAATCCGCAA
GCCAGCACCTGTGGCGAGATCGTACTCACCAGCGATTTCTATGCCTACGACACCAAGTACATTGACGAAGATGGCGCGAA
AGTGGTAGTTCCGGCAGCCATTGCACCAGAGATCAACGATAAGATCCGGGCGATTGCCGTTCAGGCTTATCAGACGTTGG
GATGCGCAGGCATGGCGCGTGTAGACGTGTTTTTAACCCCAGAGAACGAAGTGGTGATCAACGAGATCAACACACTGCCT
GGCTTCACCAACATCAGTATGTATCCGAAGCTGTGGCAAGCCAGCGGTCTGGGTTACACCGATCTGATCACACGTTTGAT
TGAGCTGGCGCTGGAGCGTCACGCTGCGGATAACGCATTGAAAACCACAATGTAA

Upstream 100 bases:

>100_bases
TTGTCAGCAGAAGATTGCAACAATCTTTCAGAAGGCCGCGAATCCCCCGTTTTGTCGTGGTAATGTATGCACTTTGTGTT
AGACGGGATAGGTTTTTAAG

Downstream 100 bases:

>100_bases
TATTTTAACGCCCGATAATATTCTTATTGGGCGTTTTCAGTTTCTTCATCGCGACGACGAATAATCAACCCCGCCAGCCA
GAAACTAATCACCCACGTCA

Product: D-alanyl-alanine synthetase A

Products: NA

Alternate protein names: D-Ala-D-Ala ligase A; D-alanylalanine synthetase A

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGIDKQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVP
GKHEHQLIDAQNGQPLPTVDVIFPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKVIVEQGIKGREIECAVLGNDNPQ
ASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAPEINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLP
GFTNISMYPKLWQASGLGYTDLITRLIELALERHAADNALKTTM

Sequences:

>Translated_364_residues
MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGIDKQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVP
GKHEHQLIDAQNGQPLPTVDVIFPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKVIVEQGIKGREIECAVLGNDNPQ
ASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAPEINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLP
GFTNISMYPKLWQASGLGYTDLITRLIELALERHAADNALKTTM
>Mature_364_residues
MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGIDKQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVP
GKHEHQLIDAQNGQPLPTVDVIFPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKVIVEQGIKGREIECAVLGNDNPQ
ASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAPEINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLP
GFTNISMYPKLWQASGLGYTDLITRLIELALERHAADNALKTTM

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786579, Length=364, Percent_Identity=100, Blast_Score=748, Evalue=0.0,
Organism=Escherichia coli, GI1786280, Length=253, Percent_Identity=39.1304347826087, Blast_Score=181, Evalue=5e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDLA_ECO57 (P0A6J9)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   C85533
- PIR:   G90682
- RefSeq:   NP_286119.1
- RefSeq:   NP_308458.1
- ProteinModelPortal:   P0A6J9
- SMR:   P0A6J9
- MINT:   MINT-1231918
- EnsemblBacteria:   EBESCT00000027690
- EnsemblBacteria:   EBESCT00000055709
- GeneID:   914533
- GeneID:   957325
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z0477
- KEGG:   ecs:ECs0431
- GeneTree:   EBGT00050000009081
- HOGENOM:   HBG427092
- OMA:   FTDISMY
- ProtClustDB:   PRK01966
- BioCyc:   ECOL83334:ECS0431-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 39316; Mature: 39316

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGIDKQGQWHVSDASNYLLN
CCCEEEEEEECCCCCCCEEHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEECCCCCEEEC
ADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVIFPIVHGTLGEDGSLQGML
CCCCCEEEECCCCHHHHHCCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCEEE
RVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFITLTRANRHNISFAEVESKLGL
EEECCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCEEEEEEEEECCCCCCCHHHHHHHCCC
PLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKVIVEQGIKGREIECAVLGNDNPQ
EEEEEECCCCCCCCCHHCCCCCCEEEEEEEEEECCCEEEECCCCCCCEEEEEEECCCCCC
ASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAPEINDKIRAIAVQAYQTLGCAGMAR
CCCCCCEEEECCEEEECCEEECCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEE
VDVFLTPENEVVINEINTLPGFTNISMYPKLWQASGLGYTDLITRLIELALERHAADNAL
EEEEECCCCCEEEEECCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHH
KTTM
HCCC
>Mature Secondary Structure
MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGIDKQGQWHVSDASNYLLN
CCCEEEEEEECCCCCCCEEHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEECCCCCEEEC
ADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVIFPIVHGTLGEDGSLQGML
CCCCCEEEECCCCHHHHHCCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCEEE
RVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFITLTRANRHNISFAEVESKLGL
EEECCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCEEEEEEEEECCCCCCCHHHHHHHCCC
PLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKVIVEQGIKGREIECAVLGNDNPQ
EEEEEECCCCCCCCCHHCCCCCCEEEEEEEEEECCCEEEECCCCCCCEEEEEEECCCCCC
ASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAPEINDKIRAIAVQAYQTLGCAGMAR
CCCCCCEEEECCEEEECCEEECCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEE
VDVFLTPENEVVINEINTLPGFTNISMYPKLWQASGLGYTDLITRLIELALERHAADNAL
EEEEECCCCCEEEEECCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHH
KTTM
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796