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The map label for this gene is yahF [H]

Identifier: 157159834

GI number: 157159834

Start: 397941

End: 399488

Strand: Direct

Name: yahF [H]

Synonym: EcHS_A0381

Alternate gene names: 157159834

Gene position: 397941-399488 (Clockwise)

Preceding gene: 157159833

Following gene: 157159835

Centisome position: 8.57

GC content: 55.23

Gene sequence:

>1548_bases
ATGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAA
ACTCGACGGCGTCGAGCAGGCATTTGTGGCGATGGCGACCGAAATGAACAAAGGTGTACTGAAAAATTTAGGACTGCTGA
CGCCGGAGCTGGAACAGGCGAAAAACGGCGACCTGATGATTGTCATCAATGGTAAATCGGGTGCGGACAACGAGCAGTTG
CTGGTGGAGATTGAAGAACTGTTCAACACCAAAGCGCAAAGCGGCTCGCACGAGGCGCGTTACGCCACTATTGCCAGCGC
CAAAAAGCATATCCCGGAAAGTAACCTGGCGGTGATTTCGGTTAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGC
TGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGTCTCAGTTGAAGATGAACTGGCGCTCAAGCAGTTGGCCCAC
GAAAAAGGGCTGCTGATGATGGGGCCGGACTGCGGCACGGCGATTATTAACGGCGCGGCGCTCTGTTTTGGTAACGCCGT
GCGTCGCGGCAATATCGGTATTGTTGGCGCATCCGGCACCGGCAGCCAGGAGCTGAGCGTCCGCATTCATGAATTTGGTG
GCGGTGTTTCACAACTGATTGGCACCGGCGGGCGCGACCTGAGCGAGAAAATCGGCGGCCTGATGATGCTCGACGCCATC
GGGATGCTGGAAAACGATCCGCAAACTGAAATTATCGCACTGATTTCAAAACCTCCCGCGCCTGCGGTGGCCCGCAAAGT
GCTGGAACGCGCACGTGCCTGCCGCAAGCCGGTGGTGGTCTGCTTCCTCGGTCGCGTTGAAACGCCGGTGGATGAGCAGG
GGCTACAGTTTGCCCGCGGCAGCAAAGAGGCGGCGCTAAAAGCGGTGATGCTCTCCGGCGTGAAACAGGAAAATCTCGAC
CTGCATACGCTTAACCAGCCGTTGATTGCTGATGTGCGTGCGCGTCTGCAACCGCAGCAGAAATACATACGTGGCCTGTT
CTGCGGCGGCACGCTGTGCGACGAAACCATGTTCGCGGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCG
ATCCTGAATTCCGCCTGCAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAAC
GGCAAGCCGCATCCAATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAAGCGCGCGATCCAGAAGTGGCGGT
GATCGTGATGGATTTTGTGCTCGGATTTGGATCGCATGAAGATCCGGTCGGCTCCACCATCGAGGCGATCAAAGAAGCGA
AAGCGATCGCCGCCGCCGAAGGACGCGAGTTGATCATTCTCGCCTATGTGCTGGGCACCGATCTCGATACGCCATCATTA
GAACAACAAAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCAGCAGCACCAATACCGGATTGCTGGCGCGTGA
ATTTATCTGCAAAGGGGAGGAAGCCTGA

Upstream 100 bases:

>100_bases
GCAATAGAAAAAATTAAACATATTGGCTCCAGTTCCGGCTGCGACATGCTGTATGGCATGGCCGATGGTTGTGCGCTGAG
CCAAACCTACGGAGGGAATT

Downstream 100 bases:

>100_bases
TGAGCCAGTCACTGTTTAGCCAACCGTTGAACGTTATTAACGTCGGTATCGCCATGTTTAGCGATGACCTGAAAAAGCAG
CATGTAGAAGTGACTCAACT

Product: acyl-CoA synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 515; Mature: 514

Protein sequence:

>515_residues
MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNKGVLKNLGLLTPELEQAKNGDLMIVINGKSGADNEQL
LVEIEELFNTKAQSGSHEARYATIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQLIGTGGRDLSEKIGGLMMLDAI
GMLENDPQTEIIALISKPPAPAVARKVLERARACRKPVVVCFLGRVETPVDEQGLQFARGSKEAALKAVMLSGVKQENLD
LHTLNQPLIADVRARLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLDFGDDDFTN
GKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAIKEAKAIAAAEGRELIILAYVLGTDLDTPSL
EQQSQMLLDAGVILASSSTNTGLLAREFICKGEEA

Sequences:

>Translated_515_residues
MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNKGVLKNLGLLTPELEQAKNGDLMIVINGKSGADNEQL
LVEIEELFNTKAQSGSHEARYATIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQLIGTGGRDLSEKIGGLMMLDAI
GMLENDPQTEIIALISKPPAPAVARKVLERARACRKPVVVCFLGRVETPVDEQGLQFARGSKEAALKAVMLSGVKQENLD
LHTLNQPLIADVRARLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLDFGDDDFTN
GKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAIKEAKAIAAAEGRELIILAYVLGTDLDTPSL
EQQSQMLLDAGVILASSSTNTGLLAREFICKGEEA
>Mature_514_residues
SVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNKGVLKNLGLLTPELEQAKNGDLMIVINGKSGADNEQLL
VEIEELFNTKAQSGSHEARYATIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAHE
KGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQLIGTGGRDLSEKIGGLMMLDAIG
MLENDPQTEIIALISKPPAPAVARKVLERARACRKPVVVCFLGRVETPVDEQGLQFARGSKEAALKAVMLSGVKQENLDL
HTLNQPLIADVRARLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLDFGDDDFTNG
KPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAIKEAKAIAAAEGRELIILAYVLGTDLDTPSLE
QQSQMLLDAGVILASSSTNTGLLAREFICKGEEA

Specific function: Unknown

COG id: COG0074

COG function: function code C; Succinyl-CoA synthetase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To E.coli fdrA and some, to bacterial sucD [H]

Homologues:

Organism=Escherichia coli, GI1786513, Length=515, Percent_Identity=98.8349514563107, Blast_Score=1036, Evalue=0.0,
Organism=Escherichia coli, GI1786729, Length=513, Percent_Identity=38.7914230019493, Blast_Score=308, Evalue=6e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003781
- InterPro:   IPR005811
- InterPro:   IPR016102 [H]

Pfam domain/function: PF02629 CoA_binding; PF00549 Ligase_CoA [H]

EC number: NA

Molecular weight: Translated: 55535; Mature: 55404

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNKGVLKNLGLLTPELEQA
CCEEEEECCCCCCCCEEEEEEHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHC
KNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYATIASAKKHIPESNLAVIS
CCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEE
VNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAHEKGLLMMGPDCGTAIINGAA
ECCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHCCCEEECCCCCHHHHCCHH
LCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQLIGTGGRDLSEKIGGLMMLDAI
HHHCCCEECCCEEEEECCCCCCCEEEEEEEHHCCCHHHHHCCCCCCHHHHHCCEEEEHHH
GMLENDPQTEIIALISKPPAPAVARKVLERARACRKPVVVCFLGRVETPVDEQGLQFARG
HHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHCHHHHCC
SKEAALKAVMLSGVKQENLDLHTLNQPLIADVRARLQPQQKYIRGLFCGGTLCDETMFAV
CHHHHHHHHHHHCCCCCCCCCEECCCHHHHHHHHHCCHHHHHHHEEECCCHHHHHHHHHH
MEKHGDVYSNIQPDPEFRLQDINRSIKHTFLDFGDDDFTNGKPHPMIDPTNRISRLIEEA
HHHCCCHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
RDPEVAVIVMDFVLGFGSHEDPVGSTIEAIKEAKAIAAAEGRELIILAYVLGTDLDTPSL
CCCCEEEEHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCH
EQQSQMLLDAGVILASSSTNTGLLAREFICKGEEA
HHHHHHHHHCCEEEEECCCCCCHHHHHHHHCCCCC
>Mature Secondary Structure 
SVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNKGVLKNLGLLTPELEQA
CEEEEECCCCCCCCEEEEEEHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHC
KNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYATIASAKKHIPESNLAVIS
CCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEE
VNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAHEKGLLMMGPDCGTAIINGAA
ECCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHCCCEEECCCCCHHHHCCHH
LCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQLIGTGGRDLSEKIGGLMMLDAI
HHHCCCEECCCEEEEECCCCCCCEEEEEEEHHCCCHHHHHCCCCCCHHHHHCCEEEEHHH
GMLENDPQTEIIALISKPPAPAVARKVLERARACRKPVVVCFLGRVETPVDEQGLQFARG
HHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHCHHHHCC
SKEAALKAVMLSGVKQENLDLHTLNQPLIADVRARLQPQQKYIRGLFCGGTLCDETMFAV
CHHHHHHHHHHHCCCCCCCCCEECCCHHHHHHHHHCCHHHHHHHEEECCCHHHHHHHHHH
MEKHGDVYSNIQPDPEFRLQDINRSIKHTFLDFGDDDFTNGKPHPMIDPTNRISRLIEEA
HHHCCCHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
RDPEVAVIVMDFVLGFGSHEDPVGSTIEAIKEAKAIAAAEGRELIILAYVLGTDLDTPSL
CCCCEEEEHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCH
EQQSQMLLDAGVILASSSTNTGLLAREFICKGEEA
HHHHHHHHHCCEEEEECCCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]