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The map label for this gene is hsdM [C]

Identifier: 157159784

GI number: 157159784

Start: 335287

End: 336495

Strand: Direct

Name: hsdM [C]

Synonym: EcHS_A0330

Alternate gene names: 157159784

Gene position: 335287-336495 (Clockwise)

Preceding gene: 157159783

Following gene: 157159785

Centisome position: 7.22

GC content: 33.17

Gene sequence:

>1209_bases
ATGAACAGTAACTATATGCCATTCACACAACGAGATAGTCTCGGAAGATACTATACAAAAGAATCCATAAGTGCATTATT
GGTTTCTCAGATGAAAGCTGAAAAAGTCAATAATATTATTGATTTAGCTTCTGGTGAGGGGAGCTTAACCTATGCTGCTT
TAGACCGATGGAAGAATGCAGAAGCATATTCTTTGGATATAGAATCTCGGATGTCAAAAAAAGTATGTGATAATCTTACT
CATATTGTAACAGATGCCCTTGTTCATTCATTTCCTGAAATGCTTGCCCGACATCAAGGAAATTTTGATGTTGCAGTATG
TAATCCTCCATTCACTCTCCCTGAATGGAGGGATGATTATTTTAAAATCATTAGTGAGATTGGCGCAGATAAATATATAT
CTGTCTCAAAATATGTCCCAGCAGAGATAATATTTATATCTCAAGTCATCAGGTTTCTTAAAAAGGGCGGTGAGGCTGGA
ATAATTTTACCTGATGGTATATTTACAGCAAGAAAGTTTATAGGTTTAAGACGATATTTATTGAATGAACATTCAATTAC
AAAAGTCATTGAATTGCCTAGGAATATCTTCAAAAGGACAGAGGCTAAAACACATATTTTAATTTTTAATAAAAAAATTA
TGCCTCATCATAAAATACAATTACATTGTATAACTAAAGATGGGGAATTGTCGCCTCCTGTTTTAATTAGAAAAGAAGAT
GCGGTTGAGAGAATGGACTACTCTTATCATTATAATAAAAATGAAGGTAAAGGGTTTAGCACAATAGGGATGCTTAAAAA
TATTTCAATTTTTAGAGGTAGGTTTAATTCAAAGGAAATTACGGAACATGTTTTTCATACGACAAAATTTAGTGGTGATG
AAAAGTACATTAAATTCCACTGCAACTCTGTAGAAGAATTGAAGCCATCAAAATTAGATGTCATTGCTAAGCCTGGTGAT
ATATTAATAGCAAGAGTTGGGCGAAATTTTCATAAAAAAATATTGTTTGTTGAGAGCGGCTATTCTTATATCAGTGACTG
TATTTTTCTGATACGAGCCTCTGGTGGAGATAAGAAAAAACTATTTGATTTTCTTTGTTCTCAAGATGGGCAAGAGGAAT
TATCTCGAGCGAGTAGTGGTGTAGCCGCACAACATATTACAATGGATGCATTAAAAAAAATACATCTTGTAAGGATTAAA
CATGACTGA

Upstream 100 bases:

>100_bases
TGAAGATAAGTTTATAAATATCAATAGATTACATGATTGTGTTGGTTCTTGGGTAGCCGTAACGCCTAGGGCGGTAGCGT
ACTTAAGATGAGTATAAAAA

Downstream 100 bases:

>100_bases
TGTGGAATTAGATAGTAATTTTCTTATCGAGGAACGAGCTGATTATATTGATGAGGATACGATAAAAAATAATACTATCA
GATCTGGAGAGTTTTTCGAT

Product: DNA methylase family protein

Products: S-Adenosyl-L-Homocysteine; DNA 6-Methylaminopurine. [C]

Alternate protein names: Type I Restriction- System M Subunit; DNA Methylase Family Protein

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MNSNYMPFTQRDSLGRYYTKESISALLVSQMKAEKVNNIIDLASGEGSLTYAALDRWKNAEAYSLDIESRMSKKVCDNLT
HIVTDALVHSFPEMLARHQGNFDVAVCNPPFTLPEWRDDYFKIISEIGADKYISVSKYVPAEIIFISQVIRFLKKGGEAG
IILPDGIFTARKFIGLRRYLLNEHSITKVIELPRNIFKRTEAKTHILIFNKKIMPHHKIQLHCITKDGELSPPVLIRKED
AVERMDYSYHYNKNEGKGFSTIGMLKNISIFRGRFNSKEITEHVFHTTKFSGDEKYIKFHCNSVEELKPSKLDVIAKPGD
ILIARVGRNFHKKILFVESGYSYISDCIFLIRASGGDKKKLFDFLCSQDGQEELSRASSGVAAQHITMDALKKIHLVRIK
HD

Sequences:

>Translated_402_residues
MNSNYMPFTQRDSLGRYYTKESISALLVSQMKAEKVNNIIDLASGEGSLTYAALDRWKNAEAYSLDIESRMSKKVCDNLT
HIVTDALVHSFPEMLARHQGNFDVAVCNPPFTLPEWRDDYFKIISEIGADKYISVSKYVPAEIIFISQVIRFLKKGGEAG
IILPDGIFTARKFIGLRRYLLNEHSITKVIELPRNIFKRTEAKTHILIFNKKIMPHHKIQLHCITKDGELSPPVLIRKED
AVERMDYSYHYNKNEGKGFSTIGMLKNISIFRGRFNSKEITEHVFHTTKFSGDEKYIKFHCNSVEELKPSKLDVIAKPGD
ILIARVGRNFHKKILFVESGYSYISDCIFLIRASGGDKKKLFDFLCSQDGQEELSRASSGVAAQHITMDALKKIHLVRIK
HD
>Mature_402_residues
MNSNYMPFTQRDSLGRYYTKESISALLVSQMKAEKVNNIIDLASGEGSLTYAALDRWKNAEAYSLDIESRMSKKVCDNLT
HIVTDALVHSFPEMLARHQGNFDVAVCNPPFTLPEWRDDYFKIISEIGADKYISVSKYVPAEIIFISQVIRFLKKGGEAG
IILPDGIFTARKFIGLRRYLLNEHSITKVIELPRNIFKRTEAKTHILIFNKKIMPHHKIQLHCITKDGELSPPVLIRKED
AVERMDYSYHYNKNEGKGFSTIGMLKNISIFRGRFNSKEITEHVFHTTKFSGDEKYIKFHCNSVEELKPSKLDVIAKPGD
ILIARVGRNFHKKILFVESGYSYISDCIFLIRASGGDKKKLFDFLCSQDGQEELSRASSGVAAQHITMDALKKIHLVRIK
HD

Specific function: The M And S Subunits Together Form A Methyltransferase (Mtase) That Methylates Two Adenine Residues In Complementary Strands Of A Bipartite DNA Recognition Sequence. In The Presence Of The R Subunit The Complex Can Also Act As An Endonuclease, Binding To

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.1.1.72 [C]

Molecular weight: Translated: 45918; Mature: 45918

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSNYMPFTQRDSLGRYYTKESISALLVSQMKAEKVNNIIDLASGEGSLTYAALDRWKNA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEHHHHHHCCCC
EAYSLDIESRMSKKVCDNLTHIVTDALVHSFPEMLARHQGNFDVAVCNPPFTLPEWRDDY
CEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHH
FKIISEIGADKYISVSKYVPAEIIFISQVIRFLKKGGEAGIILPDGIFTARKFIGLRRYL
HHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHH
LNEHSITKVIELPRNIFKRTEAKTHILIFNKKIMPHHKIQLHCITKDGELSPPVLIRKED
HCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEEEEEECCCCCCCCCEEEECHH
AVERMDYSYHYNKNEGKGFSTIGMLKNISIFRGRFNSKEITEHVFHTTKFSGDEKYIKFH
HHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEE
CNSVEELKPSKLDVIAKPGDILIARVGRNFHKKILFVESGYSYISDCIFLIRASGGDKKK
CCCHHHCCCCCEEEEECCCCEEEEECCCCCCCEEEEEECCHHHHHHHHHEEECCCCCHHH
LFDFLCSQDGQEELSRASSGVAAQHITMDALKKIHLVRIKHD
HHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHCEEEEEEECC
>Mature Secondary Structure
MNSNYMPFTQRDSLGRYYTKESISALLVSQMKAEKVNNIIDLASGEGSLTYAALDRWKNA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEHHHHHHCCCC
EAYSLDIESRMSKKVCDNLTHIVTDALVHSFPEMLARHQGNFDVAVCNPPFTLPEWRDDY
CEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHH
FKIISEIGADKYISVSKYVPAEIIFISQVIRFLKKGGEAGIILPDGIFTARKFIGLRRYL
HHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHH
LNEHSITKVIELPRNIFKRTEAKTHILIFNKKIMPHHKIQLHCITKDGELSPPVLIRKED
HCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEEEEEECCCCCCCCCEEEECHH
AVERMDYSYHYNKNEGKGFSTIGMLKNISIFRGRFNSKEITEHVFHTTKFSGDEKYIKFH
HHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEE
CNSVEELKPSKLDVIAKPGDILIARVGRNFHKKILFVESGYSYISDCIFLIRASGGDKKK
CCCHHHCCCCCEEEEECCCCEEEEECCCCCCCEEEEEECCHHHHHHHHHEEECCCCCHHH
LFDFLCSQDGQEELSRASSGVAAQHITMDALKKIHLVRIKHD
HHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-Methionine; DNA Adenine; DNA [C]

Specific reaction: S-Adenosyl-L-Methionine + DNA Adenine = S-Adenosyl-L-Homocysteine + DNA 6-Methylaminopurine. Protein + DNA = Protein-DNA [C]

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA