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The map label for this gene is rusA [H]

Identifier: 157159754

GI number: 157159754

Start: 307182

End: 307544

Strand: Direct

Name: rusA [H]

Synonym: EcHS_A0300

Alternate gene names: 157159754

Gene position: 307182-307544 (Clockwise)

Preceding gene: 157159753

Following gene: 157159755

Centisome position: 6.62

GC content: 49.59

Gene sequence:

>363_bases
ATGAACGAATATCAGTTTGTGCTTCCATACCCGCCGTCGGTGAACACCTACTGGCGAAGACGGGGAAGCCAATACTACAT
CAGCGCTAAAGGCCAGAAATACCGAAAAGATGTACAGCAAATAATCCGCCAACTCAAGTTAGACATTTTCACCAAATCAA
GACTCCGCATCAAAGTCATCGCAGACGTTCCAGACTCCCGCCGCCGCGACCTCGACAACATCCTGAAAGGTTTACTCGAC
TCCCTTATCCACGCCGGATTTGCGGAAGACGACGAGCAATTCGATGACATTCGCGTAATTCGTGGCGTGAAAGTACCAGG
CGGAAGGCTTGGAATAAAAATCACCGAACTGGAGAACGCATGA

Upstream 100 bases:

>100_bases
GACGCACCCATAATCTCGACAACAAAGACGCCAGACTTTACCACCTCGAAGGCGTGATCAGGACGCAGGCGATACTGCTG
AAGGAGGGGAAGATTAAGCC

Downstream 100 bases:

>100_bases
ACGCCACAATTCAAACGATACCAGAGCTTCTTATCCAGACACGAGGCAATCAGACCGAAGTGGCGAGGATGCTTTCCTGC
GCAAGAGGAACAGTGCTCAA

Product: crossover junction endodeoxyribonuclease RusA

Products: NA

Alternate protein names: Holliday junction nuclease rusA; Holliday junction resolvase [H]

Number of amino acids: Translated: 120; Mature: 120

Protein sequence:

>120_residues
MNEYQFVLPYPPSVNTYWRRRGSQYYISAKGQKYRKDVQQIIRQLKLDIFTKSRLRIKVIADVPDSRRRDLDNILKGLLD
SLIHAGFAEDDEQFDDIRVIRGVKVPGGRLGIKITELENA

Sequences:

>Translated_120_residues
MNEYQFVLPYPPSVNTYWRRRGSQYYISAKGQKYRKDVQQIIRQLKLDIFTKSRLRIKVIADVPDSRRRDLDNILKGLLD
SLIHAGFAEDDEQFDDIRVIRGVKVPGGRLGIKITELENA
>Mature_120_residues
MNEYQFVLPYPPSVNTYWRRRGSQYYISAKGQKYRKDVQQIIRQLKLDIFTKSRLRIKVIADVPDSRRRDLDNILKGLLD
SLIHAGFAEDDEQFDDIRVIRGVKVPGGRLGIKITELENA

Specific function: Endonuclease that resolves Holliday junction intermediates made during homologous genetic recombination and DNA repair. Exhibits sequence and structure-selective cleavage of four-way DNA junctions, where it introduces symmetrical nicks in two strands of t

COG id: COG4570

COG function: function code L; Holliday junction resolvase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rusA family [H]

Homologues:

Organism=Escherichia coli, GI1786762, Length=119, Percent_Identity=44.5378151260504, Blast_Score=107, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016281
- InterPro:   IPR008822 [H]

Pfam domain/function: PF05866 RusA [H]

EC number: =3.1.22.4 [H]

Molecular weight: Translated: 14008; Mature: 14008

Theoretical pI: Translated: 10.17; Mature: 10.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEYQFVLPYPPSVNTYWRRRGSQYYISAKGQKYRKDVQQIIRQLKLDIFTKSRLRIKVI
CCCCEEEECCCCCCCHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
ADVPDSRRRDLDNILKGLLDSLIHAGFAEDDEQFDDIRVIRGVKVPGGRLGIKITELENA
ECCCCHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCC
>Mature Secondary Structure
MNEYQFVLPYPPSVNTYWRRRGSQYYISAKGQKYRKDVQQIIRQLKLDIFTKSRLRIKVI
CCCCEEEECCCCCCCHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
ADVPDSRRRDLDNILKGLLDSLIHAGFAEDDEQFDDIRVIRGVKVPGGRLGIKITELENA
ECCCCHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA