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The map label for this gene is dinB [H]

Identifier: 157159715

GI number: 157159715

Start: 280348

End: 281403

Strand: Direct

Name: dinB [H]

Synonym: EcHS_A0260

Alternate gene names: 157159715

Gene position: 280348-281403 (Clockwise)

Preceding gene: 157159714

Following gene: 157159716

Centisome position: 6.04

GC content: 54.55

Gene sequence:

>1056_bases
ATGCGTAAAATCATTCATGTGGATATGGACTGCTTTTTCGCAGCGGTGGAGATGCGCGACAATCCCGCCCTGCGCGATAT
CCCTATTGCTATTGGCGGCAGCCGCGAACGTCGGGGGGTGATCAGCACCGCCAATTATCCCGCGCGTAAATTTGGCGTAC
GTAGCGCTATGCCGACAGGGATGGCGCTCAAATTATGCCCGCATCTCACCTTGCTTCCGGGGCGCTTTGACGCCTACAAA
GAAGCCTCAAATCATATCCGTGAAATCTTCTCGCGCTACACCTCGCGTATTGAACCGTTGTCACTGGATGAGGCTTATCT
CGACGTCACCGATAGCGTCCATTGCCACGGTTCTGCGACCCTCATCGCCCAGGAAATCCGCCAGACGATTTTCAGCGAGC
TGCAACTGACAGCGTCTGCGGGCGTGGCACCAGTAAAGTTTCTCGCCAAAATCGCCTCCGACATGAATAAACCCAACGGT
CAGTTTGTGATTACGCCGGCAGAAGTTCCGGCATTTTTACAAACCTTACCGCTGGCAAAAATCCCCGGCGTCGGCAAAGT
CTCGGCGGCAAAACTGGAAGCGATGGGGCTGCGGACCTGCGGTGATGTACAAAAGTGTGATCTGGTGATACTGCTTAAAC
GCTTTGGCAAATTTGGCCGCATTTTGTGGGAGCGTAGTCAGGGGATTGACGAGCGCGACGTTAACAGCGAACGGTTGCGA
AAATCCGTCGGCGTGGAACGCACGATGGCGGAAGATATCCACCACTGGTCTGAATGTGAAGCGATTATCGAGCGGCTGTA
TCCGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCCCGCCAGGGGGTGAAATTAAAGTTCGACG
ATTTTCAGCAAACCACTCAGGAGCACGTCTGGCCGCGGCTGAATAAAGCTGACTTAATCGCCACCGCGCGTAAAACCTGG
GATGAACGCCGCGGCGGGCGCGGTGTGCGACTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACT
GGTGCTGGGATTATGA

Upstream 100 bases:

>100_bases
CAGCAGGTGCTTTCGCAGCGAACGCGTTAAATGCTGAATCTTTACGCATTTCTCAAACTCTGAAATCACTGTATACTTTA
CCAGTGTTGAGAGGTGAGCA

Downstream 100 bases:

>100_bases
ATAACATACAAATAAGAGACTATCAGCCTGGCGATTTTCAGCAACTATGCGCTATTTTCATTAGAGCGGTTACGATGACT
GCCAGTCAGCATTATTCACC

Product: DNA polymerase IV

Products: NA

Alternate protein names: Pol IV [H]

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTANYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYK
EASNHIREIFSRYTSRIEPLSLDEAYLDVTDSVHCHGSATLIAQEIRQTIFSELQLTASAGVAPVKFLAKIASDMNKPNG
QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKFGRILWERSQGIDERDVNSERLR
KSVGVERTMAEDIHHWSECEAIIERLYPELERRLAKVKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTW
DERRGGRGVRLVGLHVTLLDPQMERQLVLGL

Sequences:

>Translated_351_residues
MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTANYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYK
EASNHIREIFSRYTSRIEPLSLDEAYLDVTDSVHCHGSATLIAQEIRQTIFSELQLTASAGVAPVKFLAKIASDMNKPNG
QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKFGRILWERSQGIDERDVNSERLR
KSVGVERTMAEDIHHWSECEAIIERLYPELERRLAKVKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTW
DERRGGRGVRLVGLHVTLLDPQMERQLVLGL
>Mature_351_residues
MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTANYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYK
EASNHIREIFSRYTSRIEPLSLDEAYLDVTDSVHCHGSATLIAQEIRQTIFSELQLTASAGVAPVKFLAKIASDMNKPNG
QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKFGRILWERSQGIDERDVNSERLR
KSVGVERTMAEDIHHWSECEAIIERLYPELERRLAKVKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTW
DERRGGRGVRLVGLHVTLLDPQMERQLVLGL

Specific function: Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by polIV. Exhibits

COG id: COG0389

COG function: function code L; Nucleotidyltransferase/DNA polymerase involved in DNA repair

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 umuC domain [H]

Homologues:

Organism=Homo sapiens, GI7705344, Length=356, Percent_Identity=33.4269662921348, Blast_Score=162, Evalue=4e-40,
Organism=Homo sapiens, GI84043967, Length=324, Percent_Identity=31.1728395061728, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI7706681, Length=325, Percent_Identity=31.3846153846154, Blast_Score=155, Evalue=6e-38,
Organism=Homo sapiens, GI154350220, Length=251, Percent_Identity=32.6693227091633, Blast_Score=135, Evalue=8e-32,
Organism=Homo sapiens, GI5729982, Length=359, Percent_Identity=30.3621169916435, Blast_Score=116, Evalue=2e-26,
Organism=Escherichia coli, GI1786425, Length=351, Percent_Identity=99.4301994301994, Blast_Score=720, Evalue=0.0,
Organism=Escherichia coli, GI1787432, Length=217, Percent_Identity=26.2672811059908, Blast_Score=79, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI193205700, Length=406, Percent_Identity=32.0197044334975, Blast_Score=172, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI17537959, Length=290, Percent_Identity=29.3103448275862, Blast_Score=120, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI193205702, Length=351, Percent_Identity=27.6353276353276, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI115534089, Length=163, Percent_Identity=34.3558282208589, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324921, Length=208, Percent_Identity=29.8076923076923, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19923006, Length=327, Percent_Identity=32.1100917431193, Blast_Score=173, Evalue=2e-43,
Organism=Drosophila melanogaster, GI21355641, Length=356, Percent_Identity=29.2134831460674, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24644984, Length=356, Percent_Identity=29.2134831460674, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24668444, Length=121, Percent_Identity=35.5371900826446, Blast_Score=75, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017962
- InterPro:   IPR017961
- InterPro:   IPR001126
- InterPro:   IPR017963
- InterPro:   IPR022880 [H]

Pfam domain/function: PF00817 IMS [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 39471; Mature: 39471

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: PS50173 UMUC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTANYPARKFGVRSAMPTG
CCCEEEECHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCEEECCCCCHHHHCCHHCCCCC
MALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSLDEAYLDVTDSVHCHGSAT
CHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCEEECCCHH
LIAQEIRQTIFSELQLTASAGVAPVKFLAKIASDMNKPNGQFVITPAEVPAFLQTLPLAK
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHCCHHH
IPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKFGRILWERSQGIDERDVNSERLR
CCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCHHHHH
KSVGVERTMAEDIHHWSECEAIIERLYPELERRLAKVKPDLLIARQGVKLKFDDFQQTTQ
HHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEHHHHHHHH
EHVWPRLNKADLIATARKTWDERRGGRGVRLVGLHVTLLDPQMERQLVLGL
HHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCHHHHHHHCCC
>Mature Secondary Structure
MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTANYPARKFGVRSAMPTG
CCCEEEECHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCEEECCCCCHHHHCCHHCCCCC
MALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSLDEAYLDVTDSVHCHGSAT
CHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCEEECCCHH
LIAQEIRQTIFSELQLTASAGVAPVKFLAKIASDMNKPNGQFVITPAEVPAFLQTLPLAK
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHCCHHH
IPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKFGRILWERSQGIDERDVNSERLR
CCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCHHHHH
KSVGVERTMAEDIHHWSECEAIIERLYPELERRLAKVKPDLLIARQGVKLKFDDFQQTTQ
HHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEHHHHHHHH
EHVWPRLNKADLIATARKTWDERRGGRGVRLVGLHVTLLDPQMERQLVLGL
HHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA