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The map label for this gene is yafL [H]

Identifier: 157159710

GI number: 157159710

Start: 276210

End: 277019

Strand: Direct

Name: yafL [H]

Synonym: EcHS_A0255

Alternate gene names: 157159710

Gene position: 276210-277019 (Clockwise)

Preceding gene: 157159706

Following gene: 157159714

Centisome position: 5.95

GC content: 52.1

Gene sequence:

>810_bases
TTGTTCAGGTACGGGCCGGGATACACTGCAACATCAATGCATGATCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCC
CTCTTTTGTGTTGTCGGGATTACTGTTGCTTTGCTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCACTT
TCAGCTACGCCGCCCGCCAGCGGATGCAAAATCGTGCGCGTTTATTAAAACAGTACCAAACCCATCTGAAAAAACAGGCC
AGCTATATTGTGGAAGGCAATGCCGAAAGCAGAAGGGCGCTACGCCAGCACAACCGCGAACAGATAAAACAGCATCCAGA
ATGGTTTCCTGCGCCGCTCAAGGCGAGTGACAGACGCTGGCAGGCGCTGGCGGAAAACAACCATTTCTTAAGCAGCGACC
ATCTGCATAACATCACCGAAGTGGCGATTCACCGTCTGGAGCAACAGCTTGGCAAGCCTTACGTCTGGGGCGGTACGCGG
CCCGATCAAGGCTTCGACTGTAGCGGGTTAGTTTTTTATGCCTACAACAAGATCCTTGAGGCGAAACTCCCACGCACGGC
TAATGAGATGTATCACTATCGCCGGGCAACCATTGTGGCGAATAACGACCTGCGCCGGGGAGATTTGCTATTTTTCCATA
TCCACAGCCGCGAGATTGCCGACCATATGGGGGTGTATCTGGGCGACGGTCAGTTTATTGAGTCACCGCGCACCGGGGAA
ACCATTCGGGTAAGTCAGTTAGCTGAACCTTTCTGGCAGGATCACTTTTTAGGCGCGCGGCGGATTTTGACGGAGGAGAC
GATTTTGTAA

Upstream 100 bases:

>100_bases
ATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATTTATTAATTTT
ATAATAATGGTTTATTAAGT

Downstream 100 bases:

>100_bases
ATGGGTAATATTGACGAACTGGCAAAAGGTCGGATGCCACGCGGCATCCGACCTGTTTACATCTGGGCTGAAAACTCACG
TTCTAAGACATCAACAATGA

Product: NlpC/P60 family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MFRYGPGYTATSMHDQPLLSMSLPSIPSFVLSGLLLLCLPFSSFASATTSHITFSYAARQRMQNRARLLKQYQTHLKKQA
SYIVEGNAESRRALRQHNREQIKQHPEWFPAPLKASDRRWQALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTR
PDQGFDCSGLVFYAYNKILEAKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRTGE
TIRVSQLAEPFWQDHFLGARRILTEETIL

Sequences:

>Translated_269_residues
MFRYGPGYTATSMHDQPLLSMSLPSIPSFVLSGLLLLCLPFSSFASATTSHITFSYAARQRMQNRARLLKQYQTHLKKQA
SYIVEGNAESRRALRQHNREQIKQHPEWFPAPLKASDRRWQALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTR
PDQGFDCSGLVFYAYNKILEAKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRTGE
TIRVSQLAEPFWQDHFLGARRILTEETIL
>Mature_269_residues
MFRYGPGYTATSMHDQPLLSMSLPSIPSFVLSGLLLLCLPFSSFASATTSHITFSYAARQRMQNRARLLKQYQTHLKKQA
SYIVEGNAESRRALRQHNREQIKQHPEWFPAPLKASDRRWQALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTR
PDQGFDCSGLVFYAYNKILEAKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRTGE
TIRVSQLAEPFWQDHFLGARRILTEETIL

Specific function: Unknown

COG id: COG0791

COG function: function code M; Cell wall-associated hydrolases (invasion-associated proteins)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nlpC/p60 family [H]

Homologues:

Organism=Escherichia coli, GI1786421, Length=249, Percent_Identity=97.5903614457831, Blast_Score=513, Evalue=1e-147,
Organism=Escherichia coli, GI1787944, Length=134, Percent_Identity=52.2388059701493, Blast_Score=178, Evalue=3e-46,
Organism=Escherichia coli, GI1788501, Length=114, Percent_Identity=32.4561403508772, Blast_Score=68, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000064 [H]

Pfam domain/function: PF00877 NLPC_P60 [H]

EC number: NA

Molecular weight: Translated: 31040; Mature: 31040

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRYGPGYTATSMHDQPLLSMSLPSIPSFVLSGLLLLCLPFSSFASATTSHITFSYAARQ
CCCCCCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
RMQNRARLLKQYQTHLKKQASYIVEGNAESRRALRQHNREQIKQHPEWFPAPLKASDRRW
HHHHHHHHHHHHHHHHHHHHCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
QALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNKILE
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCHHHHHHHHHHH
AKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRTGE
HHCCHHHHHHHHHHHEEEEECCCCCCCCEEEEEECCHHHHHHCCEEECCCCEECCCCCCC
TIRVSQLAEPFWQDHFLGARRILTEETIL
EEEHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFRYGPGYTATSMHDQPLLSMSLPSIPSFVLSGLLLLCLPFSSFASATTSHITFSYAARQ
CCCCCCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
RMQNRARLLKQYQTHLKKQASYIVEGNAESRRALRQHNREQIKQHPEWFPAPLKASDRRW
HHHHHHHHHHHHHHHHHHHHCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
QALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNKILE
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCHHHHHHHHHHH
AKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRTGE
HHCCHHHHHHHHHHHEEEEECCCCCCCCEEEEEECCHHHHHHCCEEECCCCEECCCCCCC
TIRVSQLAEPFWQDHFLGARRILTEETIL
EEEHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7596361; 9278503 [H]