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The map label for this gene is yafS [H]

Identifier: 157159674

GI number: 157159674

Start: 234415

End: 235137

Strand: Direct

Name: yafS [H]

Synonym: EcHS_A0217

Alternate gene names: 157159674

Gene position: 234415-235137 (Clockwise)

Preceding gene: 157159671

Following gene: 157159676

Centisome position: 5.05

GC content: 49.24

Gene sequence:

>723_bases
ATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAGAGCTTTATCGCAA
GGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAG
AAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTGCGCAAGGAATGCCCGTCCAGGTACAGGCGGAC
CCACTTCATCTTCCTTTTGCCGATAAATCCGTTGATGTTTGTCTACTGGCACATACATTGCCGTGGTGCACCGATCCGCA
TCGTTTATTGCGTGAAGCCGATCGGGTATTGATTGATGATGGCTGGCTGGTGATTAGTGGCTTCAATCCCATCAGTTTAA
TGGGATTACGCAAACTTGTGCCGGTATTGCGCAAAACCTCGCCCTATAACAGCCGGATGTTTACTCTGATGCGGCAACTG
GACTGGCTCTCTTTGTTGAATTTTGAAGTGCTGCACGCCAGCCGTTTCCACGTTCTCCCGTGGAACAAACACGGTGGAAA
ACTATTGAATGCACATATTCCTGCGCTTGGTTGCTTACAACTTATTGTTGCCCGGAAACGGACTATTCCTTTAACGCTAA
ATCCGATGAAACAGAGTAAAAACAAGCCACGAATTCGCCAGGCGGTTGGAGCCACCCGGCAATATCGTAAACCACAGGCT
TAA

Upstream 100 bases:

>100_bases
GACCTGCTTCATCATTCAAAACCCAGATGTAATTGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTTA
GTGTGAAACGGAAGGTTGTG

Downstream 100 bases:

>100_bases
ACTTCGACTTGATAGCCTGTATCTTCCAGTGTGGGATTCATTGCCGCAGCACGGGCCAGTTCATCACAGCGTTCGTTTTC
CGGGTGTCCGGCATGGCCTT

Product: putative methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFHLLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQAD
PLHLPFADKSVDVCLLAHTLPWCTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQSKNKPRIRQAVGATRQYRKPQA

Sequences:

>Translated_240_residues
MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFHLLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQAD
PLHLPFADKSVDVCLLAHTLPWCTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQSKNKPRIRQAVGATRQYRKPQA
>Mature_240_residues
MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFHLLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQAD
PLHLPFADKSVDVCLLAHTLPWCTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQSKNKPRIRQAVGATRQYRKPQA

Specific function: Unknown

COG id: COG0500

COG function: function code QR; SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87081700, Length=240, Percent_Identity=98.75, Blast_Score=488, Evalue=1e-139,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013216 [H]

Pfam domain/function: PF08241 Methyltransf_11 [H]

EC number: NA

Molecular weight: Translated: 27271; Mature: 27271

Theoretical pI: Translated: 10.54; Mature: 10.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFHLLKIGNLSAEINCEAC
CCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHEEEEEECCCCEEECCCEE
AVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPWCTDPHRLLREADRVLIDD
EEEEEECCCCCCCEEEEECCCEECCCCCCCCCEEEEECCCCCCCCHHHHHHHHCEEEEEC
GWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQLDWLSLLNFEVLHASRFHVLP
CEEEEECCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHEEECCEEEEEC
WNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQSKNKPRIRQAVGATRQYRKPQA
CCCCCCEEEECCCCHHHHHHHHHHHCCCCEEEECHHHHCCCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFHLLKIGNLSAEINCEAC
CCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHEEEEEECCCCEEECCCEE
AVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPWCTDPHRLLREADRVLIDD
EEEEEECCCCCCCEEEEECCCEECCCCCCCCCEEEEECCCCCCCCHHHHHHHHCEEEEEC
GWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQLDWLSLLNFEVLHASRFHVLP
CEEEEECCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHEEECCEEEEEC
WNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQSKNKPRIRQAVGATRQYRKPQA
CCCCCCEEEECCCCHHHHHHHHHHHCCCCEEEECHHHHCCCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]